2D and 3D Cellular Screening Models and AI Guided Analysis, Volume 204 explores the development, application, and comparative benefits of 2D monolayer cultures and 3D organoids, spheroids, and tissue-engineered constructs for biomedical research and drug discovery. Chapters in this new release include High throughput CellTiter-glo viability assessment, Assessing viability of cancer cell spheroids in 3D drug screening, 3D killing assay of cancer spheroids by cytotoxic T lymphocytes in anchored microfluidic droplets, Use of micropatterning for 3D cell culture, Use of microfluidic chip devices for assessing the effect of radiation on 3D cell culture models, and much more.
Additional chapters cover A 3D model for assessing regulated cell death modalities, YOLO detection model for Cell Viability assessment, Bioinformatics and AI/ML approaches using multi-omics data to accelerate diagnosis and delivery of precision care, Automated image analysis and machine learning for the assessment of mitochondrial membrane potential, Assessing cancer cell heterogeneity in patient-derived tumoroids, Assessment of lysosomal drug sequestration and release using fluorescence microscopy, Assessment of T cell toxicity in ex-vivo models of cancer, Three-dimensional Organoid Screening using Leica Thunder Microscope, Using vascularized organoids-on-chip for assessing systemic drug delivery, and many more interesting and timely topics.
1. Title of Book - p. i 2. Cover image - p. i 3. Title page - p. ii 4. Table of Contents - p. iii 5. Series Page - p. iii 6. Copyright - p. iv 7. Contributors - p. xv 8. 2D and 3D cellular screening models and AI guided analysis - p. xxi 9. Chapter One: High-throughput cell viability assessment using a luminescent ATP detection assay - p. 1 10. Chapter Two: Assessing spheroid viability in high throughput - p. 13 11. Chapter Three: 3D killing assay of cancer spheroids by cytotoxic T lymphocytes in anchored microfluidic droplets - p. 27 12. Chapter Four: Engineering microcavity arrays as a niche for high-throughput and high-content analysis of tumoroids: A detailed methodology - p. 41 13. Chapter Five: Use of microfluidic “chip” devices for assessing the effect of radiation on 3D cell culture models - p. 53 14. Chapter Six: A 3D model for assessing regulated cell death modalities - p. 81 15. Chapter Seven: Single-cell viability assessment using YOLOv8n object detection - p. 93 16. Chapter Eight: Bioinformatics and AI/ML approaches using multi-omics data to accelerate diagnosis and delivery of precision care for patients with rare diseases - p. 109 17. Chapter Nine: High-content microscopy quantification of mitochondrial membrane potential - p. 141 18. Chapter Ten: Assessing cancer cell heterogeneity in patient-derived tumoroids - p. 155 19. Chapter Eleven: Assessment of lysosomal drug sequestration and release using fluorescence microscopy - p. 171 20. Chapter Twelve: A preclinical organotypic model for evaluating immunotherapy-related toxicities - p. 193 21. Chapter Thirteen: Three-dimensional organoid screening using a Leica Thunder microscope - p. 205 22. Chapter Fourteen: Using vascularized organoids-on-chip for assessing systemic drug delivery - p. 237 23. Chapter Fifteen: Quantitative assessment of nanoparticle uptake and trafficking in advanced 3D cell models: A high-content screening core to enhance precision nanomedicine development - p. 245 24. Chapter Sixteen: Fluorescence microscopic quantification of lysosomal cholesterol accumulation induced by cationic amphiphilic drugs - p. 285
2D and 3D Cellular Screening Models and AI Guided Analysis, Volume 204 explores the development, application, and comparative benefits of 2D monolayer cultures and 3D organoids, spheroids, and tissue-engineered constructs for biomedical research and drug discovery. Chapters in this new release include High throughput CellTiter-glo viability assessment, Assessing viability of cancer cell spheroids in 3D drug screening, 3D killing assay of cancer spheroids by cytotoxic T lymphocytes in anchored microfluidic droplets, Use of micropatterning for 3D cell culture, Use of microfluidic chip devices for assessing the effect of radiation on 3D cell culture models, and much more.
Additional chapters cover A 3D model for assessing regulated cell death modalities, YOLO detection model for Cell Viability assessment, Bioinformatics and AI/ML approaches using multi-omics data to accelerate diagnosis and delivery of precision care, Automated image analysis and machine learning for the assessment of mitochondrial membrane potential, Assessing cancer cell heterogeneity in patient-derived tumoroids, Assessment of lysosomal drug sequestration and release using fluorescence microscopy, Assessment of T cell toxicity in ex-vivo models of cancer, Three-dimensional Organoid Screening using Leica Thunder Microscope, Using vascularized organoids-on-chip for assessing systemic drug delivery, and many more interesting and timely topics.
1. Title of Book - p. i 2. Cover image - p. i 3. Title page - p. ii 4. Table of Contents - p. iii 5. Series Page - p. iii 6. Copyright - p. iv 7. Contributors - p. xv 8. 2D and 3D cellular screening models and AI guided analysis - p. xxi 9. Chapter One: High-throughput cell viability assessment using a luminescent ATP detection assay - p. 1 10. Chapter Two: Assessing spheroid viability in high throughput - p. 13 11. Chapter Three: 3D killing assay of cancer spheroids by cytotoxic T lymphocytes in anchored microfluidic droplets - p. 27 12. Chapter Four: Engineering microcavity arrays as a niche for high-throughput and high-content analysis of tumoroids: A detailed methodology - p. 41 13. Chapter Five: Use of microfluidic “chip” devices for assessing the effect of radiation on 3D cell culture models - p. 53 14. Chapter Six: A 3D model for assessing regulated cell death modalities - p. 81 15. Chapter Seven: Single-cell viability assessment using YOLOv8n object detection - p. 93 16. Chapter Eight: Bioinformatics and AI/ML approaches using multi-omics data to accelerate diagnosis and delivery of precision care for patients with rare diseases - p. 109 17. Chapter Nine: High-content microscopy quantification of mitochondrial membrane potential - p. 141 18. Chapter Ten: Assessing cancer cell heterogeneity in patient-derived tumoroids - p. 155 19. Chapter Eleven: Assessment of lysosomal drug sequestration and release using fluorescence microscopy - p. 171 20. Chapter Twelve: A preclinical organotypic model for evaluating immunotherapy-related toxicities - p. 193 21. Chapter Thirteen: Three-dimensional organoid screening using a Leica Thunder microscope - p. 205 22. Chapter Fourteen: Using vascularized organoids-on-chip for assessing systemic drug delivery - p. 237 23. Chapter Fifteen: Quantitative assessment of nanoparticle uptake and trafficking in advanced 3D cell models: A high-content screening core to enhance precision nanomedicine development - p. 245 24. Chapter Sixteen: Fluorescence microscopic quantification of lysosomal cholesterol accumulation induced by cationic amphiphilic drugs - p. 285
