Advanced Methodologies to Study and Enhance Cellular Cytotoxicity Against Cancer, Volume 207 covers the latest techniques and strategies used to investigate and boost cytotoxic activity in cells targeting cancer. Chapters include An ex vivo assay to assess CAR T cell cytotoxicity in viable tumor tissues using a two-step staining protocol, New protocol to measure mitochondria polarization/function at the immunological synapse, Analysis of CAR-T cell–mediated cytotoxicity in solid tumor models, Investigation of the in vitro lytic capacity of CAR-T by microscopyal, Antigen-specific T cell cytotoxicity toward target tumor cells, In vitro expansion of stem-like memory T cells via D-mannose supplementation, and much more.
Additional chapters explore High-throughput time-lapse imaging of B16 target cell killing by ovalbumin-specific CD8+ T cells, Evaluation of an intracellular magneto-mechanical approach for regulating dendritic cell activation, Analyzing the effect of NK cells on in-vivo models of tumor dissemination by digital pathology and flow cytometry, Super-resolution imaging of the NK cell synapse on supported lipid bilayers using MINFLUX nanoscopy, ASSESSMENT OF NK CELL CYTOTOXICITY INDUCED BY IL-15 BASED IMMUNOTHERAPY AGAINST CANCER CELLS, Combined phenotyping and functional analysis of isolated human natural killer cells using spectral flow cytometry, and much more.
1. Title of Book - p. i 2. Cover image - p. i 3. Title page - p. ii 4. Table of Contents - p. iii 5. Series page - p. iii 6. Copyright - p. iv 7. Contributors - p. xiii 8. Chapter One: An ex vivo assay to assess CAR T cell cytotoxicity in viable tumor tissues using a two-step staining protocol - p. 1 9. Chapter Two: Image analysis of mitochondria during T lymphocyte and chimeric antigen receptor (CAR) effector responses - p. 19 10. Chapter Three: Analysis of murine CAR-T cell–mediated cytotoxicity in solid tumor models - p. 53 11. Chapter Four: Live-Cell Microscopy for quantitative analysis of CAR T Cell fratricide and immune synapse - p. 75 12. Chapter Five: Modelling antigen-specific T cell-mediated killing of cancer cells in vitro - p. 95 13. Chapter Six: In vitro expansion of stem-like memory T cells via D-mannose supplementation - p. 107 14. Chapter Seven: High-throughput time-lapse imaging of B16 target cell killing by ovalbumin-specific CD8+ T cells - p. 119 15. Chapter Eight: Evaluation of an intracellular magneto-mechanical approach for regulating dendritic cell activation - p. 139 16. Chapter Nine: Analyzing the effect of NK cells on in-vivo models of tumor dissemination by digital pathology and flow cytometry - p. 155 17. Chapter Ten: MINFLUX nanoscopy to study the NK cell immune synapse - p. 179 18. Chapter Eleven: Assessment of NK cell cytotoxicity induced by IL-15 based immunotherapy against cancer cells - p. 203 19. Chapter Twelve: Combined phenotyping and functional analysis of isolated human natural killer cells using spectral flow cytometry - p. 219 20. Chapter Thirteen: Characterization of anti-tumor activity in different NK cell subsets by multiparametric flow cytometry - p. 237 21. Chapter Fourteen: DNA origami-bilayer systems for dissecting the nanoscale antigen landscape in B cell immune synapse formation - p. 255
Advanced Methodologies to Study and Enhance Cellular Cytotoxicity Against Cancer, Volume 207 covers the latest techniques and strategies used to investigate and boost cytotoxic activity in cells targeting cancer. Chapters include An ex vivo assay to assess CAR T cell cytotoxicity in viable tumor tissues using a two-step staining protocol, New protocol to measure mitochondria polarization/function at the immunological synapse, Analysis of CAR-T cell–mediated cytotoxicity in solid tumor models, Investigation of the in vitro lytic capacity of CAR-T by microscopyal, Antigen-specific T cell cytotoxicity toward target tumor cells, In vitro expansion of stem-like memory T cells via D-mannose supplementation, and much more.
Additional chapters explore High-throughput time-lapse imaging of B16 target cell killing by ovalbumin-specific CD8+ T cells, Evaluation of an intracellular magneto-mechanical approach for regulating dendritic cell activation, Analyzing the effect of NK cells on in-vivo models of tumor dissemination by digital pathology and flow cytometry, Super-resolution imaging of the NK cell synapse on supported lipid bilayers using MINFLUX nanoscopy, ASSESSMENT OF NK CELL CYTOTOXICITY INDUCED BY IL-15 BASED IMMUNOTHERAPY AGAINST CANCER CELLS, Combined phenotyping and functional analysis of isolated human natural killer cells using spectral flow cytometry, and much more.
1. Title of Book - p. i 2. Cover image - p. i 3. Title page - p. ii 4. Table of Contents - p. iii 5. Series page - p. iii 6. Copyright - p. iv 7. Contributors - p. xiii 8. Chapter One: An ex vivo assay to assess CAR T cell cytotoxicity in viable tumor tissues using a two-step staining protocol - p. 1 9. Chapter Two: Image analysis of mitochondria during T lymphocyte and chimeric antigen receptor (CAR) effector responses - p. 19 10. Chapter Three: Analysis of murine CAR-T cell–mediated cytotoxicity in solid tumor models - p. 53 11. Chapter Four: Live-Cell Microscopy for quantitative analysis of CAR T Cell fratricide and immune synapse - p. 75 12. Chapter Five: Modelling antigen-specific T cell-mediated killing of cancer cells in vitro - p. 95 13. Chapter Six: In vitro expansion of stem-like memory T cells via D-mannose supplementation - p. 107 14. Chapter Seven: High-throughput time-lapse imaging of B16 target cell killing by ovalbumin-specific CD8+ T cells - p. 119 15. Chapter Eight: Evaluation of an intracellular magneto-mechanical approach for regulating dendritic cell activation - p. 139 16. Chapter Nine: Analyzing the effect of NK cells on in-vivo models of tumor dissemination by digital pathology and flow cytometry - p. 155 17. Chapter Ten: MINFLUX nanoscopy to study the NK cell immune synapse - p. 179 18. Chapter Eleven: Assessment of NK cell cytotoxicity induced by IL-15 based immunotherapy against cancer cells - p. 203 19. Chapter Twelve: Combined phenotyping and functional analysis of isolated human natural killer cells using spectral flow cytometry - p. 219 20. Chapter Thirteen: Characterization of anti-tumor activity in different NK cell subsets by multiparametric flow cytometry - p. 237 21. Chapter Fourteen: DNA origami-bilayer systems for dissecting the nanoscale antigen landscape in B cell immune synapse formation - p. 255
