Biocatalysis, Volume 714 provides a wide range of themes dealing with the identification and application of biocatalysts. This includes various formats such as whole-cell or cell-free biocatalysis as well as immobilized variants. Specific chapters in this new release include Biocatalysis: How to select the proper mode of application, Documentation in biocatalysis and data handling, Mining metagenomes from extremophiles as resource for novel glycoside hydrolases for industrial applications, Functional Metaproteomics, Sequence-function relation for the prediction of enzyme properties: A case study on flavin-dependent oxidases, P450 monooxygenase in whole-cell format, and more.
Additional sections cover Regio- and Stereospecific oxidation based on di-iron monooxygenases producing whole cells, Recombinant enzyme expression and targeted mutagenesis in Aromatoleum species, C.necator as a model organism for CO2-based biotechnology, Cupriavidus in whole-cell biocatalysis, Whole-cell biocatalysis with Myxobacteria, Streptomyces for natural product formation: Targeted mutagenesis in PKS, Reductive Amination: Methods for cell-free and whole-cell biocatalysis, Challenges and good practices on transaminase-catalysed synthesis of optically pure amines, W-enzymes in biocatalysis: Chances and difficulties for the user, Atroposelective biocatalysis employing ADHs, Applications of alcohol oxidases, and much more.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Series Page - p. iii 5. Copyright - p. iv 6. Contributors - p. xix 7. Preface - p. xxvii 8. Chapter One: Biocatalysis: Important considerations for testing and evaluation of biocatalysts - p. 1 9. Chapter Two: Digitalization of biocatalysis: Best practices to research data management - p. 19 10. Chapter Three: Mining metagenomes from extremophiles as a resource for novel glycoside hydrolases for industrial applications - p. 45 11. Chapter Four: Functional metaproteomics for enzyme discovery - p. 61 12. Chapter Five: Sequence-function relation for the prediction of enzyme properties: A case study on flavin-dependent oxidases - p. 83 13. Chapter Six: Cytochrome P450 monooxygenases in whole-cell format: Application notes from a biotechnological perspective - p. 97 14. Chapter Seven: Whole-cell biocatalysis for epoxidation using the non-heme diiron monooxygenase system PmlABCDEF - p. 115 15. Chapter Eight: Genetic manipulation of the betaproteobacterial genera Thauera and Aromatoleum - p. 139 16. Chapter Nine: Cupriavidus necator as a model organism for CO2-based biotechnology - p. 163 17. Chapter Ten: Enzyme expression in Cupriavidus necator H16 for whole-cell biocatalysis - p. 195 18. Chapter Eleven: Whole-cell biocatalysis with Myxococcus xanthus - p. 219 19. Chapter Twelve: How to engineer giant enzymes: A methodology for mutagenesis of polyketide synthases in native hosts - p. 239 20. Chapter Thirteen: Reductive amination: Methods for cell-free and whole-cell biocatalysis - p. 269 21. Chapter Fourteen: Challenges and good practices on transaminase-catalysed synthesis of optically pure amines - p. 297 22. Chapter Fifteen: Tungsten containing aldehyde oxidoreductase (AOR)-family enzymes; past, present and future production strategies - p. 313 23. Chapter Sixteen: Atroposelective biocatalysis employing alcohol dehydrogenases - p. 337 24. Chapter Seventeen: Discovery, production and characterization of bacterial aryl-alcohol oxidases - p. 355 25. Chapter Eighteen: Use of xylose reductase as a cofactor enhancing system for in vivo biocatalysis - p. 379 26. Chapter Nineteen: Design of fusion proteins for biocatalysis - p. 393 27. Chapter Twenty: Engineering unspecific peroxygenases by structure-guided in vivo recombination of homologous protein blocks - p. 407 28. Chapter Twenty One: Peroxygenase-catalysed oxyfunctionalisation reactions - p. 425 29. Chapter Twenty Two: Cell-free protein synthesis for biocatalysis - p. 445 30. Chapter Twenty Three: Immobilisation for a stable biocatalyst application - p. 465 31. Chapter Twenty Four: Ultrahigh-throughput screening assay for PET-degrading enzymes - p. 489
Biocatalysis, Volume 714 provides a wide range of themes dealing with the identification and application of biocatalysts. This includes various formats such as whole-cell or cell-free biocatalysis as well as immobilized variants. Specific chapters in this new release include Biocatalysis: How to select the proper mode of application, Documentation in biocatalysis and data handling, Mining metagenomes from extremophiles as resource for novel glycoside hydrolases for industrial applications, Functional Metaproteomics, Sequence-function relation for the prediction of enzyme properties: A case study on flavin-dependent oxidases, P450 monooxygenase in whole-cell format, and more.
Additional sections cover Regio- and Stereospecific oxidation based on di-iron monooxygenases producing whole cells, Recombinant enzyme expression and targeted mutagenesis in Aromatoleum species, C.necator as a model organism for CO2-based biotechnology, Cupriavidus in whole-cell biocatalysis, Whole-cell biocatalysis with Myxobacteria, Streptomyces for natural product formation: Targeted mutagenesis in PKS, Reductive Amination: Methods for cell-free and whole-cell biocatalysis, Challenges and good practices on transaminase-catalysed synthesis of optically pure amines, W-enzymes in biocatalysis: Chances and difficulties for the user, Atroposelective biocatalysis employing ADHs, Applications of alcohol oxidases, and much more.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Series Page - p. iii 5. Copyright - p. iv 6. Contributors - p. xix 7. Preface - p. xxvii 8. Chapter One: Biocatalysis: Important considerations for testing and evaluation of biocatalysts - p. 1 9. Chapter Two: Digitalization of biocatalysis: Best practices to research data management - p. 19 10. Chapter Three: Mining metagenomes from extremophiles as a resource for novel glycoside hydrolases for industrial applications - p. 45 11. Chapter Four: Functional metaproteomics for enzyme discovery - p. 61 12. Chapter Five: Sequence-function relation for the prediction of enzyme properties: A case study on flavin-dependent oxidases - p. 83 13. Chapter Six: Cytochrome P450 monooxygenases in whole-cell format: Application notes from a biotechnological perspective - p. 97 14. Chapter Seven: Whole-cell biocatalysis for epoxidation using the non-heme diiron monooxygenase system PmlABCDEF - p. 115 15. Chapter Eight: Genetic manipulation of the betaproteobacterial genera Thauera and Aromatoleum - p. 139 16. Chapter Nine: Cupriavidus necator as a model organism for CO2-based biotechnology - p. 163 17. Chapter Ten: Enzyme expression in Cupriavidus necator H16 for whole-cell biocatalysis - p. 195 18. Chapter Eleven: Whole-cell biocatalysis with Myxococcus xanthus - p. 219 19. Chapter Twelve: How to engineer giant enzymes: A methodology for mutagenesis of polyketide synthases in native hosts - p. 239 20. Chapter Thirteen: Reductive amination: Methods for cell-free and whole-cell biocatalysis - p. 269 21. Chapter Fourteen: Challenges and good practices on transaminase-catalysed synthesis of optically pure amines - p. 297 22. Chapter Fifteen: Tungsten containing aldehyde oxidoreductase (AOR)-family enzymes; past, present and future production strategies - p. 313 23. Chapter Sixteen: Atroposelective biocatalysis employing alcohol dehydrogenases - p. 337 24. Chapter Seventeen: Discovery, production and characterization of bacterial aryl-alcohol oxidases - p. 355 25. Chapter Eighteen: Use of xylose reductase as a cofactor enhancing system for in vivo biocatalysis - p. 379 26. Chapter Nineteen: Design of fusion proteins for biocatalysis - p. 393 27. Chapter Twenty: Engineering unspecific peroxygenases by structure-guided in vivo recombination of homologous protein blocks - p. 407 28. Chapter Twenty One: Peroxygenase-catalysed oxyfunctionalisation reactions - p. 425 29. Chapter Twenty Two: Cell-free protein synthesis for biocatalysis - p. 445 30. Chapter Twenty Three: Immobilisation for a stable biocatalyst application - p. 465 31. Chapter Twenty Four: Ultrahigh-throughput screening assay for PET-degrading enzymes - p. 489
