Biochemical Pathways and Environmental Responses in Plants: Part C, Volume 683 in the Methods in Enzymology series, highlights advances in the field. with this new volume presenting chapters on topics including Preparation of hydroxycinnamoyl-CoA thioesters using recombinant 4-coumarate:coenzyme A ligase (4CL) for characterization of BAHD acyltransferases, Near-real time determination of BAHD acyl-coA transferase reaction rates, Bioinformatic tools for protein structure prediction and for molecular docking applied to enzyme active site analysis, Computational and biochemical methods to measure the activity of carboxysomes and protein organelles in vivo, Dirigent Proteins Family Function and Structure, Three-in-One Method for High-throughput plant multi-omics, amongst other timely topics.
Additional sections cover Analysis of isoprenyl-phosphates by liquid chromatography-mass spectrometry, Lignin/Lignan Biosynthesis Structure/Function, Comparison of TLC, HPLC, and direct infusion mass spectrometry methods for identification and quantification of diacylglycerol molecular species, Plant sphingolipid analysis, RNA-seq analysis of alternative pre-mRNA splicing mediated by photoreceptors in Physcomitrium patens, and much more.
1. Cover image - p. i 2. Table of Contents - p. iii 3. Series Page - p. iii 4. Copyright - p. iii 5. Contributors - p. iii 6. Preface - p. xv 7. Section I: Protein - p. xvi 8. Chapter One: Preparation of hydroxycinnamoyl-coenzyme A thioesters using recombinant 4-coumarate:coenzyme A ligase (4CL) for characterization of BAHD hydroxycinnamoyltransferase enzyme activities - p. 3 9. Chapter Two: Near-real time determination of BAHD acyl-coenzyme A transferase reaction rates and kinetic parameters using Ellman's reagent - p. 19 10. Chapter Three: Tools for protein structure prediction and for molecular docking applied to enzyme active site analysis: A case study using a BAHD hydroxycinnamoyltransferase - p. 46 11. Chapter Four: Computational and biochemical methods to measure the activity of carboxysomes and protein organelles in vivo - p. 81 12. Chapter Five: Dirigent protein subfamily function and structure in terrestrial plant phenol metabolism - p. 101 13. Section II: Metabolite - p. 150 14. Chapter Six: Three-in-one method for high throughput plant multi-omics - p. 153 15. Chapter Seven: Analysis of isoprenyl-phosphates by liquid chromatography-mass spectrometry - p. 171 16. Chapter Eight: Comparison of TLC, HPLC, and direct-infusion ESI-MS methods for the identification and quantification of diacylglycerol molecular species - p. 205 17. Section III: Environment - p. 224 18. Chapter Nine: RNA-seq analysis of alternative pre-mRNA splicing regulation mediated by photoreceptors in Physcomitrium patens - p. 227 19. Chapter Ten: Monitoring lipid-protein interactions in planta using Förster resonance energy transfer - p. 243 20. Chapter Eleven: Acetylene reduction assay for nitrogenase activity in root nodules under salinity stress - p. 253 21. Chapter Twelve: Using Hyper as a molecular probe to visualize hydrogen peroxide in living plant cells: An updated method - p. 265 22. Chapter Thirteen: Using DCP-Rho1 as a fluorescent probe to visualize sulfenic acid-containing proteins in living plant cells - p. 291
Biochemical Pathways and Environmental Responses in Plants: Part C, Volume 683 in the Methods in Enzymology series, highlights advances in the field. with this new volume presenting chapters on topics including Preparation of hydroxycinnamoyl-CoA thioesters using recombinant 4-coumarate:coenzyme A ligase (4CL) for characterization of BAHD acyltransferases, Near-real time determination of BAHD acyl-coA transferase reaction rates, Bioinformatic tools for protein structure prediction and for molecular docking applied to enzyme active site analysis, Computational and biochemical methods to measure the activity of carboxysomes and protein organelles in vivo, Dirigent Proteins Family Function and Structure, Three-in-One Method for High-throughput plant multi-omics, amongst other timely topics.
Additional sections cover Analysis of isoprenyl-phosphates by liquid chromatography-mass spectrometry, Lignin/Lignan Biosynthesis Structure/Function, Comparison of TLC, HPLC, and direct infusion mass spectrometry methods for identification and quantification of diacylglycerol molecular species, Plant sphingolipid analysis, RNA-seq analysis of alternative pre-mRNA splicing mediated by photoreceptors in Physcomitrium patens, and much more.
1. Cover image - p. i 2. Table of Contents - p. iii 3. Series Page - p. iii 4. Copyright - p. iii 5. Contributors - p. iii 6. Preface - p. xv 7. Section I: Protein - p. xvi 8. Chapter One: Preparation of hydroxycinnamoyl-coenzyme A thioesters using recombinant 4-coumarate:coenzyme A ligase (4CL) for characterization of BAHD hydroxycinnamoyltransferase enzyme activities - p. 3 9. Chapter Two: Near-real time determination of BAHD acyl-coenzyme A transferase reaction rates and kinetic parameters using Ellman's reagent - p. 19 10. Chapter Three: Tools for protein structure prediction and for molecular docking applied to enzyme active site analysis: A case study using a BAHD hydroxycinnamoyltransferase - p. 46 11. Chapter Four: Computational and biochemical methods to measure the activity of carboxysomes and protein organelles in vivo - p. 81 12. Chapter Five: Dirigent protein subfamily function and structure in terrestrial plant phenol metabolism - p. 101 13. Section II: Metabolite - p. 150 14. Chapter Six: Three-in-one method for high throughput plant multi-omics - p. 153 15. Chapter Seven: Analysis of isoprenyl-phosphates by liquid chromatography-mass spectrometry - p. 171 16. Chapter Eight: Comparison of TLC, HPLC, and direct-infusion ESI-MS methods for the identification and quantification of diacylglycerol molecular species - p. 205 17. Section III: Environment - p. 224 18. Chapter Nine: RNA-seq analysis of alternative pre-mRNA splicing regulation mediated by photoreceptors in Physcomitrium patens - p. 227 19. Chapter Ten: Monitoring lipid-protein interactions in planta using Förster resonance energy transfer - p. 243 20. Chapter Eleven: Acetylene reduction assay for nitrogenase activity in root nodules under salinity stress - p. 253 21. Chapter Twelve: Using Hyper as a molecular probe to visualize hydrogen peroxide in living plant cells: An updated method - p. 265 22. Chapter Thirteen: Using DCP-Rho1 as a fluorescent probe to visualize sulfenic acid-containing proteins in living plant cells - p. 291
