Biofilms, Volume 53 in the ongoing Methods in Microbiology series, highlights new advances in the field with this new volume presenting interesting chapters on a variety of timely topics, including Monospecies and polymicrobial biofilms in static and flow environment, Methods used to study biofilms, Spatial analysis of bacterial biofilms, Biofilm grown in bioreactors, Single-cell analysis of subpopulations within biofilms using microscopy, flow cytometry, and imaging flow cytometry, Microscopy analysis of biofilm-mineral interactions or mineral formation within biofilms, Bacterial biofilms as an essential component of rhizosphere plant-microbe interactions, Studying Gene Expression in Biofilms, and more.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Copyright - p. iii 5. Contributors - p. iii 6. Preface - p. xvii 7. Section 1: Plant biofilms - p. xviii 8. Chapter 1: Bacterial biofilms as an essential component of rhizosphere plant-microbe interactions - p. 12 9. Section 2: Mixed species - p. 48 10. Chapter 2: Monospecies and polymicrobial biofilms in static and flow environment - p. 51 11. Chapter 3: Optimization of protocol for quantification of biofilm formed by pathogenic rapidly-growing nontuberculous mycobacteria for diagnostic screening - p. 67 12. Chapter 4: Investigating inter-kingdom interaction between oral Streptococcus mutans and Candida species in mixed-species biofilms - p. 101 13. Section 3: Nano techniques - p. 120 14. Chapter 5: Programming bacterial adhesion to functionalized surfaces through cellular display of recombinant nanobodies - p. 123 15. Chapter 6: Micro- and nanoscale techniques for studying biofilm-mineral interactions - p. 174 16. Section 4: Genetics and microfluidics - p. 192 17. Chapter 7: Methods for studying biofilms: Microfluidics and translation in the clinical context - p. 206 18. Chapter 8: Studying gene expression in biofilms - p. 247 19. Section 5: Microsocopy - p. 271 20. Chapter 9: Spatial analysis of multispecies bacterial biofilms - p. 275 21. Chapter 10: Use of epifluorescence widefield deconvolution microscopy for imaging and three-dimensional rendering of Pseudomonas aeruginosa biofilms and extracellular matrix materials - p. 309
Biofilms, Volume 53 in the ongoing Methods in Microbiology series, highlights new advances in the field with this new volume presenting interesting chapters on a variety of timely topics, including Monospecies and polymicrobial biofilms in static and flow environment, Methods used to study biofilms, Spatial analysis of bacterial biofilms, Biofilm grown in bioreactors, Single-cell analysis of subpopulations within biofilms using microscopy, flow cytometry, and imaging flow cytometry, Microscopy analysis of biofilm-mineral interactions or mineral formation within biofilms, Bacterial biofilms as an essential component of rhizosphere plant-microbe interactions, Studying Gene Expression in Biofilms, and more.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Copyright - p. iii 5. Contributors - p. iii 6. Preface - p. xvii 7. Section 1: Plant biofilms - p. xviii 8. Chapter 1: Bacterial biofilms as an essential component of rhizosphere plant-microbe interactions - p. 12 9. Section 2: Mixed species - p. 48 10. Chapter 2: Monospecies and polymicrobial biofilms in static and flow environment - p. 51 11. Chapter 3: Optimization of protocol for quantification of biofilm formed by pathogenic rapidly-growing nontuberculous mycobacteria for diagnostic screening - p. 67 12. Chapter 4: Investigating inter-kingdom interaction between oral Streptococcus mutans and Candida species in mixed-species biofilms - p. 101 13. Section 3: Nano techniques - p. 120 14. Chapter 5: Programming bacterial adhesion to functionalized surfaces through cellular display of recombinant nanobodies - p. 123 15. Chapter 6: Micro- and nanoscale techniques for studying biofilm-mineral interactions - p. 174 16. Section 4: Genetics and microfluidics - p. 192 17. Chapter 7: Methods for studying biofilms: Microfluidics and translation in the clinical context - p. 206 18. Chapter 8: Studying gene expression in biofilms - p. 247 19. Section 5: Microsocopy - p. 271 20. Chapter 9: Spatial analysis of multispecies bacterial biofilms - p. 275 21. Chapter 10: Use of epifluorescence widefield deconvolution microscopy for imaging and three-dimensional rendering of Pseudomonas aeruginosa biofilms and extracellular matrix materials - p. 309
