Biomolecular Interactions: Part A, Volume 166, the latest release in the Methods in Cell Biology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of timely topics in cell biology. Each chapter is written by an international board of authors.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Series Page - p. iii 5. Copyright - p. iii 6. Contributors - p. iii 7. Preface - p. iii 8. Chapter 1: Measuring the rapid kinetics of receptor-ligand interactions in live cells using NanoBRET - p. 1 9. Chapter 2: Evaluating functional ligand-GPCR interactions in cell-based assays - p. 15 10. Chapter 3: Assays for detecting arrestin interaction with GPCRs - p. 43 11. Chapter 4: BRET-based assay to specifically monitor β2AR/GRK2 interaction and β-arrestin2 conformational change upon βAR stimulation - p. 67 12. Chapter 5: Cannabinoid receptor CB1 and CB2 interacting proteins: Techniques, progress and perspectives - p. 83 13. Chapter 6: Purinergic GPCR transmembrane residues involved in ligand recognition and dimerization - p. 139 14. Chapter 7: Nanobodies as sensors of GPCR activation and signaling - p. 161 15. Chapter 8: Confocal and TIRF microscopy based approaches to visualize arrestin trafficking in living cells - p. 179 16. Chapter 9: Strategies for targeting cell surface proteins using multivalent conjugates and chemical biology - p. 205 17. Chapter 10: Identifying Plasmodium falciparum receptor activation using bioluminescence resonance energy transfer (BRET)-based biosensors in HEK293 cells - p. 223 18. Chapter 11: Methods for binding analysis of small GTP-binding proteins with their effectors - p. 235 19. Chapter 12: Investigating protein expression, modifications and interactions in the brain: Protocol for preparing rodent brain tissue for mass spectrometry-based quantitative- and phospho-proteomics analysis - p. 251 20. Chapter 13: Protein-protein interactions at a glance: Protocols for the visualization of biomolecular interactions - p. 271 21. Chapter 14: Interactions between noncoding RNAs as epigenetic regulatory mechanisms in cardiovascular diseases - p. 322
Biomolecular Interactions: Part A, Volume 166, the latest release in the Methods in Cell Biology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of timely topics in cell biology. Each chapter is written by an international board of authors.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Series Page - p. iii 5. Copyright - p. iii 6. Contributors - p. iii 7. Preface - p. iii 8. Chapter 1: Measuring the rapid kinetics of receptor-ligand interactions in live cells using NanoBRET - p. 1 9. Chapter 2: Evaluating functional ligand-GPCR interactions in cell-based assays - p. 15 10. Chapter 3: Assays for detecting arrestin interaction with GPCRs - p. 43 11. Chapter 4: BRET-based assay to specifically monitor β2AR/GRK2 interaction and β-arrestin2 conformational change upon βAR stimulation - p. 67 12. Chapter 5: Cannabinoid receptor CB1 and CB2 interacting proteins: Techniques, progress and perspectives - p. 83 13. Chapter 6: Purinergic GPCR transmembrane residues involved in ligand recognition and dimerization - p. 139 14. Chapter 7: Nanobodies as sensors of GPCR activation and signaling - p. 161 15. Chapter 8: Confocal and TIRF microscopy based approaches to visualize arrestin trafficking in living cells - p. 179 16. Chapter 9: Strategies for targeting cell surface proteins using multivalent conjugates and chemical biology - p. 205 17. Chapter 10: Identifying Plasmodium falciparum receptor activation using bioluminescence resonance energy transfer (BRET)-based biosensors in HEK293 cells - p. 223 18. Chapter 11: Methods for binding analysis of small GTP-binding proteins with their effectors - p. 235 19. Chapter 12: Investigating protein expression, modifications and interactions in the brain: Protocol for preparing rodent brain tissue for mass spectrometry-based quantitative- and phospho-proteomics analysis - p. 251 20. Chapter 13: Protein-protein interactions at a glance: Protocols for the visualization of biomolecular interactions - p. 271 21. Chapter 14: Interactions between noncoding RNAs as epigenetic regulatory mechanisms in cardiovascular diseases - p. 322
