Cell Death Part B continues the comprehensive exploration of cellular mechanisms governing the fate of cells in health and disease. This volume delves deeper into the molecular pathways, regulatory networks, and biological consequences of various cell death modalities, including apoptosis, necrosis, autophagy, ferroptosis, pyroptosis, and other emerging forms.
1. Title of Book - p. i 2. Cover image - p. i 3. Title page - p. ii 4. Table of Contents - p. iii 5. Series Page - p. iii 6. Copyright - p. iv 7. Contributors - p. xiii 8. Chapter One: Techniques for monitoring and isolating lipid droplets in cell death - p. 1 9. Chapter Two: Semi-automated, quantitative immunofluorescence analysis of single cell cytochrome c release during apoptotic cell death - p. 23 10. Chapter Three: Purification and analysis of cell death complexes - p. 43 11. Chapter Four: Functional assessment and quantitative kinetic analysis of BAX activation using large unilamellar vesicles - p. 55 12. Chapter Five: Evaluation of lipid peroxidation as a marker of ferroptosis in colon cancer cells - p. 81 13. Chapter Six: Detecting ferroptotic cells using an antibody against hyperoxidized PRDX3 - p. 95 14. Chapter Seven: Measurement and quantification of macrophage lytic cell death by kinetic microscopy - p. 109 15. Chapter Eight: Quantifying loss of cell viability or adhesion using methylene blue - p. 123 16. Chapter Nine: Protocols for MCF-7 derived mammosphere generation and anti-cancer drug testing - p. 143 17. Chapter Ten: Transmission electron microscopy protocol for characterising autophagy-dependent cell death (ADCD) in Drosophila melanogaster larval midguts - p. 153 18. Chapter Eleven: Measuring in vitro induction of antigen-specific CD8+ T-cell responses by murine conventional dendritic cells - p. 169 19. Chapter Twelve: Isolation and maintenance of commonly used primary cells for cell death research - p. 199 20. Chapter Thirteen: Cell death detection in iPSC-derived cortical neurons as model of neurodegeneration - p. 215
Cell Death Part B continues the comprehensive exploration of cellular mechanisms governing the fate of cells in health and disease. This volume delves deeper into the molecular pathways, regulatory networks, and biological consequences of various cell death modalities, including apoptosis, necrosis, autophagy, ferroptosis, pyroptosis, and other emerging forms.
1. Title of Book - p. i 2. Cover image - p. i 3. Title page - p. ii 4. Table of Contents - p. iii 5. Series Page - p. iii 6. Copyright - p. iv 7. Contributors - p. xiii 8. Chapter One: Techniques for monitoring and isolating lipid droplets in cell death - p. 1 9. Chapter Two: Semi-automated, quantitative immunofluorescence analysis of single cell cytochrome c release during apoptotic cell death - p. 23 10. Chapter Three: Purification and analysis of cell death complexes - p. 43 11. Chapter Four: Functional assessment and quantitative kinetic analysis of BAX activation using large unilamellar vesicles - p. 55 12. Chapter Five: Evaluation of lipid peroxidation as a marker of ferroptosis in colon cancer cells - p. 81 13. Chapter Six: Detecting ferroptotic cells using an antibody against hyperoxidized PRDX3 - p. 95 14. Chapter Seven: Measurement and quantification of macrophage lytic cell death by kinetic microscopy - p. 109 15. Chapter Eight: Quantifying loss of cell viability or adhesion using methylene blue - p. 123 16. Chapter Nine: Protocols for MCF-7 derived mammosphere generation and anti-cancer drug testing - p. 143 17. Chapter Ten: Transmission electron microscopy protocol for characterising autophagy-dependent cell death (ADCD) in Drosophila melanogaster larval midguts - p. 153 18. Chapter Eleven: Measuring in vitro induction of antigen-specific CD8+ T-cell responses by murine conventional dendritic cells - p. 169 19. Chapter Twelve: Isolation and maintenance of commonly used primary cells for cell death research - p. 199 20. Chapter Thirteen: Cell death detection in iPSC-derived cortical neurons as model of neurodegeneration - p. 215
