Cell Death: Part E, Volume 210 presents the latest advances and comprehensive reviews in the rapidly evolving field of cell death. This volume explores the molecular mechanisms, regulatory pathways, and biological consequences of various forms of cell death—including apoptosis, necrosis, autophagy, and other non-canonical processes. Chapters in this new release include Flow cytometry analysis of mitochondrial apoptotic priming with BH3 profiling on senescent cells, Methods to study PIDDOsome and caspase 2 signaling, Isolation, characterization, and experimental applications of apoptotic bodies in vitro, Lipid ROS Measurement by Spectral Flow Cytometry based on the ratiometric dye BODIPYTM C11 581/591, and much more.
Additional chapters cover CRISPR-Cas9 Screen to Identify Genes Regulating Cell Death, Flow cytometry-assisted discrimination of early apoptotic, late apoptotic and primary necrotic morphological markers in Saccharomyces cerevisiae, Cytofluorometric analysis of cell death in primary human neutrophils, Real-time tracking of cell death in human intestinal organoids: a quantitative imaging approach, and more.
1. Title of Book - p. i 2. Cover image - p. i 3. Title page - p. ii 4. Table of Contents - p. iii 5. Series page - p. iii 6. Copyright - p. iv 7. Contributors - p. xi 8. Chapter One: Flow cytometry analysis of mitochondrial apoptotic priming with BH3 profiling on senescent cells - p. 1 9. Chapter Two: Methods to study PIDDosome and caspase-2 signalling - p. 17 10. Chapter Three: Isolation, characterization, and experimental applications of apoptotic bodies in vitro - p. 41 11. Chapter Four: Lipid ROS measurement by spectral flow cytometry based on the ratiometric dye BODIPY™ C11 581/591 - p. 61 12. Chapter Five: CRISPR-Cas9 screen to identify genes regulating cell death - p. 83 13. Chapter Six: Flow cytometric distinction of early apoptotic, late apoptotic and primary necrotic morphological markers in Saccharomyces cerevisiae - p. 97 14. Chapter Seven: Cytofluorometric analysis of cell death in primary human neutrophils - p. 115 15. Chapter Eight: Real-time tracking of cell death in human intestinal organoids: A quantitative imaging approach - p. 137 16. Chapter Nine: Short-term culture of human endoscopic specimens for the study of chronic inflammation - p. 157 17. Chapter Ten: In vitro protocols for inducing and assessing endotoxin tolerance in human and murine macrophages - p. 171 18. Chapter Eleven: Assessment of microglial responsiveness after irradiation in mouse organotypic hippocampus or patient-derived tissue cultures - p. 189
Cell Death: Part E, Volume 210 presents the latest advances and comprehensive reviews in the rapidly evolving field of cell death. This volume explores the molecular mechanisms, regulatory pathways, and biological consequences of various forms of cell death—including apoptosis, necrosis, autophagy, and other non-canonical processes. Chapters in this new release include Flow cytometry analysis of mitochondrial apoptotic priming with BH3 profiling on senescent cells, Methods to study PIDDOsome and caspase 2 signaling, Isolation, characterization, and experimental applications of apoptotic bodies in vitro, Lipid ROS Measurement by Spectral Flow Cytometry based on the ratiometric dye BODIPYTM C11 581/591, and much more.
Additional chapters cover CRISPR-Cas9 Screen to Identify Genes Regulating Cell Death, Flow cytometry-assisted discrimination of early apoptotic, late apoptotic and primary necrotic morphological markers in Saccharomyces cerevisiae, Cytofluorometric analysis of cell death in primary human neutrophils, Real-time tracking of cell death in human intestinal organoids: a quantitative imaging approach, and more.
1. Title of Book - p. i 2. Cover image - p. i 3. Title page - p. ii 4. Table of Contents - p. iii 5. Series page - p. iii 6. Copyright - p. iv 7. Contributors - p. xi 8. Chapter One: Flow cytometry analysis of mitochondrial apoptotic priming with BH3 profiling on senescent cells - p. 1 9. Chapter Two: Methods to study PIDDosome and caspase-2 signalling - p. 17 10. Chapter Three: Isolation, characterization, and experimental applications of apoptotic bodies in vitro - p. 41 11. Chapter Four: Lipid ROS measurement by spectral flow cytometry based on the ratiometric dye BODIPY™ C11 581/591 - p. 61 12. Chapter Five: CRISPR-Cas9 screen to identify genes regulating cell death - p. 83 13. Chapter Six: Flow cytometric distinction of early apoptotic, late apoptotic and primary necrotic morphological markers in Saccharomyces cerevisiae - p. 97 14. Chapter Seven: Cytofluorometric analysis of cell death in primary human neutrophils - p. 115 15. Chapter Eight: Real-time tracking of cell death in human intestinal organoids: A quantitative imaging approach - p. 137 16. Chapter Nine: Short-term culture of human endoscopic specimens for the study of chronic inflammation - p. 157 17. Chapter Ten: In vitro protocols for inducing and assessing endotoxin tolerance in human and murine macrophages - p. 171 18. Chapter Eleven: Assessment of microglial responsiveness after irradiation in mouse organotypic hippocampus or patient-derived tissue cultures - p. 189
