Cell Polarity and Morphogenesis, the latest volume in the Methods in Cell Biology series, looks at cell polarity and morphogenesis. Edited by leaders in the field, this volume provides proven, state-of-art techniques, along with relevant historical background and theory, to aid researchers in efficient design and effective implementation of experimental methodologies.
1. Cover image - p. i 2. Title page - p. i 3. Table of Contents - p. iii 4. Series Editors - p. ii 5. Copyright - p. iv 6. Contributors - p. ix 7. Chapter 1. A user's guide for characterizing plasma membrane subdomains in living cells by spot variation fluorescence correlation spectroscopy - p. 1 8. Chapter 2. Investigating symmetry breaking in yeast: From seeing to understanding - p. 23 9. Chapter 3. Precise tracking of the dynamics of multiple proteins in endocytic events - p. 51 10. Chapter 4. Quantitative approaches for the study of microtubule aster motion in large eggs - p. 69 11. Chapter 5. Dissection and characterization of microtubule bundles in the mitotic spindle using femtosecond laser ablation - p. 81 12. Chapter 6. Fluorescence eXclusion Measurement of volume in live cells - p. 103 13. Chapter 7. Quantitative methods to study epithelial morphogenesis and polarity - p. 121 14. Chapter 8. Probing tissue interaction with laser-based cauterization in the early developing Drosophila embryo - p. 153 15. Chapter 9. Optogenetic inhibition of apical constriction during Drosophila embryonic development - p. 167 16. Chapter 10. Micropipette aspiration: A unique tool for exploring cell and tissue mechanics in vivo - p. 187 17. Chapter 11. In-vivo analysis of morphogenesis in plants - p. 203
Cell Polarity and Morphogenesis, the latest volume in the Methods in Cell Biology series, looks at cell polarity and morphogenesis. Edited by leaders in the field, this volume provides proven, state-of-art techniques, along with relevant historical background and theory, to aid researchers in efficient design and effective implementation of experimental methodologies.
1. Cover image - p. i 2. Title page - p. i 3. Table of Contents - p. iii 4. Series Editors - p. ii 5. Copyright - p. iv 6. Contributors - p. ix 7. Chapter 1. A user's guide for characterizing plasma membrane subdomains in living cells by spot variation fluorescence correlation spectroscopy - p. 1 8. Chapter 2. Investigating symmetry breaking in yeast: From seeing to understanding - p. 23 9. Chapter 3. Precise tracking of the dynamics of multiple proteins in endocytic events - p. 51 10. Chapter 4. Quantitative approaches for the study of microtubule aster motion in large eggs - p. 69 11. Chapter 5. Dissection and characterization of microtubule bundles in the mitotic spindle using femtosecond laser ablation - p. 81 12. Chapter 6. Fluorescence eXclusion Measurement of volume in live cells - p. 103 13. Chapter 7. Quantitative methods to study epithelial morphogenesis and polarity - p. 121 14. Chapter 8. Probing tissue interaction with laser-based cauterization in the early developing Drosophila embryo - p. 153 15. Chapter 9. Optogenetic inhibition of apical constriction during Drosophila embryonic development - p. 167 16. Chapter 10. Micropipette aspiration: A unique tool for exploring cell and tissue mechanics in vivo - p. 187 17. Chapter 11. In-vivo analysis of morphogenesis in plants - p. 203
