The imaging of small cellular components requires powerful instruments, and an entire family of equipment and techniques based on the confocal principle has been developed over the past 30 years. Such methods are commonly used by neuroscience researchers, but the majority of these users do not have a microscopy or a cell biology backgrounds and do can encounter difficulties in obtaining and interpreting results. This volume brings experts in high-resolution optical microscopy applications in neuroscience and cell biology together to document the state of the art. Outlining what is currently possible, the volume also discusses promising developments for the future and aids readers in selecting the most scientifically meaningful approach to solve their questions. Each chapter discusses instrumentation and technology in relationship to application in research. All of the common and cutting edge trends are covered - fluorescence / laser electron / nonlinear microscopy, infrared fluorescence, multiphoton imaging, tomography, FRAP, live imaging, STED, PALM/STORM, etc.
1. Cover image 2. Title page 3. Table of Contents 4. Copyright 5. List of Contributors 6. Chapter 1. Confocal Laser Scanning: of Instrument, Computer Processing, and Men 7. Chapter 2. Beyond Abbe’s Resolution Barrier 8. Chapter 3. Enhancement of Optical Resolution by 4pi Single and Multiphoton Confocal Fluorescence Microscopy 9. Chapter 4. Nano Resolution Optical Imaging Through Localization Microscopy 10. Chapter 5. Optical Investigation of Brain Networks Using Structured Illumination 11. Chapter 6. Multiphoton Microscopy Advances Toward Super Resolution 12. Chapter 7. The Cell at Molecular Resolution 13. Chapter 8. Cellular-Level Optical Biopsy Using Full-Field Optical Coherence Microscopy 14. Chapter 9. Retroviral Labeling and Imaging of Newborn Neurons in the Adult Brain 15. Chapter 10. Study of Myelin Sheaths by Cars Microscopy 16. Chapter 11. High-Resolution Approaches to Studying Presynaptic Vesicle Dynamics Using Variants of Frap and Electron Microscopy 17. Index
The imaging of small cellular components requires powerful instruments, and an entire family of equipment and techniques based on the confocal principle has been developed over the past 30 years. Such methods are commonly used by neuroscience researchers, but the majority of these users do not have a microscopy or a cell biology backgrounds and do can encounter difficulties in obtaining and interpreting results. This volume brings experts in high-resolution optical microscopy applications in neuroscience and cell biology together to document the state of the art. Outlining what is currently possible, the volume also discusses promising developments for the future and aids readers in selecting the most scientifically meaningful approach to solve their questions. Each chapter discusses instrumentation and technology in relationship to application in research. All of the common and cutting edge trends are covered - fluorescence / laser electron / nonlinear microscopy, infrared fluorescence, multiphoton imaging, tomography, FRAP, live imaging, STED, PALM/STORM, etc.
1. Cover image 2. Title page 3. Table of Contents 4. Copyright 5. List of Contributors 6. Chapter 1. Confocal Laser Scanning: of Instrument, Computer Processing, and Men 7. Chapter 2. Beyond Abbe’s Resolution Barrier 8. Chapter 3. Enhancement of Optical Resolution by 4pi Single and Multiphoton Confocal Fluorescence Microscopy 9. Chapter 4. Nano Resolution Optical Imaging Through Localization Microscopy 10. Chapter 5. Optical Investigation of Brain Networks Using Structured Illumination 11. Chapter 6. Multiphoton Microscopy Advances Toward Super Resolution 12. Chapter 7. The Cell at Molecular Resolution 13. Chapter 8. Cellular-Level Optical Biopsy Using Full-Field Optical Coherence Microscopy 14. Chapter 9. Retroviral Labeling and Imaging of Newborn Neurons in the Adult Brain 15. Chapter 10. Study of Myelin Sheaths by Cars Microscopy 16. Chapter 11. High-Resolution Approaches to Studying Presynaptic Vesicle Dynamics Using Variants of Frap and Electron Microscopy 17. Index
