Cellular Senescence and Aging, Volume 181 in the Methods in Cell Biology series, highlights new advances in the field, with this new volume presenting interesting chapters on topics such as assessing polyglutamine tract aggregation in the nematode Caenorhabditis elegans, Generation of glial cell-derived neurotrophic factor (gdnf) morphants in zebrafish larvae by cerebroventricular microinjection of vivo morpholino, Methods for detection of mitochondrial reactive oxygen species in senescent cells, Assessment of cell cycle progression and mitotic slippage by videomicroscopy, The original colorimetric method to detect cellular senescence, and more.
Additional sections cover Assessing microbiota composition in the context of aging, Assessing chronological aging in Saccharomyces cerevisiae, Image processing and supervised machine learning for retinal microglia characterization in senescence, Measuring telomerase activity using TRAP assays, High throughput assessment of cellular senescence, Detection of radiation-induced senescence by the Debacq-Chainiaux protocol: Improvements and upgrade in the detection of positive events, Dynamic and scalable assessment of the senescence-associated secretory phenotype (SASP), Flow cytometry-assisted quantification of cell cycle arrest in cancer cells treated with CDK4/6 inhibitors, and more.
1. Cover image - p. i 2. Table of Contents - p. iii 3. Series Page - p. iii 4. Copyright - p. iii 5. Contributors - p. iii 6. Cellular senescence and aging at the crossroad between immunity and cancer - p. xvii 7. Chapter 1: Assessing polyglutamine tract aggregation in the nematode Caenorhabditis elegans - p. 1 8. Chapter 2: Generation of glial cell-derived neurotrophic factor (gdnf) morphants in zebrafish larvae by cerebroventricular microinjection of vivo morpholino - p. 17 9. Chapter 3: Methods for detection of mitochondrial reactive oxygen species in senescent cells - p. 33 10. Chapter 4: Assessment of cell cycle progression and mitotic slippage by videomicroscopy - p. 43 11. Chapter 5: The original colorimetric method to detect cellular senescence - p. 59 12. Chapter 6: Assessing microbiota composition in the context of aging - p. 73 13. Chapter 7: Assessing chronological aging in Saccharomyces cerevisiae - p. 87 14. Chapter 8: Image processing and supervised machine learning for retinal microglia characterization in senescence - p. 117 15. Chapter 9: Measuring telomerase activity using TRAP assays - p. 127 16. Chapter 10: High-throughput assessment of cellular senescence - p. 151 17. Chapter 11: Detection of radiation-induced senescence by the Debacq-Chainiaux protocol: Improvements and upgrade in the detection of positive events - p. 161 18. Chapter 12: Dynamic and scalable assessment of the senescence-associated secretory phenotype (SASP) - p. 181 19. Chapter 13: Flow cytometry-assisted quantification of cell cycle arrest in cancer cells treated with CDK4/6 inhibitors - p. 197 20. Chapter 14: Mechanistic exploration of autophagy and aging by RNA interference - p. 213
Cellular Senescence and Aging, Volume 181 in the Methods in Cell Biology series, highlights new advances in the field, with this new volume presenting interesting chapters on topics such as assessing polyglutamine tract aggregation in the nematode Caenorhabditis elegans, Generation of glial cell-derived neurotrophic factor (gdnf) morphants in zebrafish larvae by cerebroventricular microinjection of vivo morpholino, Methods for detection of mitochondrial reactive oxygen species in senescent cells, Assessment of cell cycle progression and mitotic slippage by videomicroscopy, The original colorimetric method to detect cellular senescence, and more.
Additional sections cover Assessing microbiota composition in the context of aging, Assessing chronological aging in Saccharomyces cerevisiae, Image processing and supervised machine learning for retinal microglia characterization in senescence, Measuring telomerase activity using TRAP assays, High throughput assessment of cellular senescence, Detection of radiation-induced senescence by the Debacq-Chainiaux protocol: Improvements and upgrade in the detection of positive events, Dynamic and scalable assessment of the senescence-associated secretory phenotype (SASP), Flow cytometry-assisted quantification of cell cycle arrest in cancer cells treated with CDK4/6 inhibitors, and more.
1. Cover image - p. i 2. Table of Contents - p. iii 3. Series Page - p. iii 4. Copyright - p. iii 5. Contributors - p. iii 6. Cellular senescence and aging at the crossroad between immunity and cancer - p. xvii 7. Chapter 1: Assessing polyglutamine tract aggregation in the nematode Caenorhabditis elegans - p. 1 8. Chapter 2: Generation of glial cell-derived neurotrophic factor (gdnf) morphants in zebrafish larvae by cerebroventricular microinjection of vivo morpholino - p. 17 9. Chapter 3: Methods for detection of mitochondrial reactive oxygen species in senescent cells - p. 33 10. Chapter 4: Assessment of cell cycle progression and mitotic slippage by videomicroscopy - p. 43 11. Chapter 5: The original colorimetric method to detect cellular senescence - p. 59 12. Chapter 6: Assessing microbiota composition in the context of aging - p. 73 13. Chapter 7: Assessing chronological aging in Saccharomyces cerevisiae - p. 87 14. Chapter 8: Image processing and supervised machine learning for retinal microglia characterization in senescence - p. 117 15. Chapter 9: Measuring telomerase activity using TRAP assays - p. 127 16. Chapter 10: High-throughput assessment of cellular senescence - p. 151 17. Chapter 11: Detection of radiation-induced senescence by the Debacq-Chainiaux protocol: Improvements and upgrade in the detection of positive events - p. 161 18. Chapter 12: Dynamic and scalable assessment of the senescence-associated secretory phenotype (SASP) - p. 181 19. Chapter 13: Flow cytometry-assisted quantification of cell cycle arrest in cancer cells treated with CDK4/6 inhibitors - p. 197 20. Chapter 14: Mechanistic exploration of autophagy and aging by RNA interference - p. 213
