Cellulase refers to a class of enzymes produced chiefly by fungi, bacteria, and protozoans that catalyze cellulolysis. This volume of Methods in Enzymology comprehensively covers this topic. With an international board of authors, this volume covers subjects such as "The DNSA reducing assay for measuring cellulases," "Measuring processivity" and "In situ cellulose detection with carbohydrate-binding modules."
1. Cover image 2. Title page 3. Table of Contents 4. Series Page 5. Copyright 6. Contributors 7. Preface 8. Methods in Enzymology 9. Chapter one: Measurement of endo-1,4-β-Glucanase 10. Chapter two: Biomass Conversion Determined via Fluorescent Cellulose Decay Assay 11. Chapter three: The Analysis of Saccharification in Biomass Using an Automated High-Throughput Method 12. Chapter four: Studies of Enzymatic Cleavage of Cellulose Using Polysaccharide Analysis by Carbohydrate gel Electrophoresis (PACE) 13. Chapter five: Measuring Processivity 14. Chapter six: Distinguishing Xyloglucanase Activity in endo-β(1 → 4)glucanases 15. Chapter seven: Methods for Structural Characterization of the Products of Cellulose- and Xyloglucan-Hydrolyzing Enzymes 16. Chapter eight: The Crystallization and Structural Analysis of Cellulases (and Other Glycoside Hydrolases) 17. Chapter nine: Visualization of Cellobiohydrolase I from Trichoderma reesei Moving on Crystalline Cellulose Using High-Speed Atomic Force Microscopy 18. Chapter ten: Small-Angle X-ray Scattering and Crystallography 19. Chapter eleven: Quantitative Approaches to The Analysis of Carbohydrate-Binding Module Function 20. Chapter twelve: In Situ Detection of Cellulose with Carbohydrate-Binding Modules 21. Chapter thirteen: Interactions Between Family 3 Carbohydrate Binding Modules (CBMs) and Cellulosomal Linker Peptides 22. Chapter fourteen: Approaches for Improving Thermostability Characteristics in Cellulases 23. Chapter fifteen: Thermophilic Glycosynthases for Oligosaccharides Synthesis 24. Chapter sixteen: Engineering Cellulase Activity into Clostridium acetobutylicum 25. Chapter seventeen: Transformation of Clostridium Thermocellum by Electroporation 26. Chapter eighteen: Genetic and Functional Genomic Approaches for the Study of Plant Cell Wall Degradation in Cellvibrio japonicus 27. Chapter nineteen: Methods for the Isolation of Cellulose-Degrading Microorganisms 28. Chapter twenty: Metagenomic Approaches to the Discovery of Cellulases 29. Chapter twenty-one: Escherichia coli Expression, Purification, Crystallization, and Structure Determination of Bacterial Cohesin–Dockerin Complexes 30. Chapter twenty-two: Measurements of Relative Binding of Cohesin and Dockerin Mutants Using an Advanced ELISA Technique for High-Affinity Interactions 31. Chapter twenty-three: Designer Cellulosomes for Enhanced Hydrolysis of Cellulosic Substrates 32. Chapter twenty-four: High-Throughput Screening of Cohesin Mutant Libraries on Cellulose Microarrays 33. Author Index 34. Subject Index
Cellulase refers to a class of enzymes produced chiefly by fungi, bacteria, and protozoans that catalyze cellulolysis. This volume of Methods in Enzymology comprehensively covers this topic. With an international board of authors, this volume covers subjects such as "The DNSA reducing assay for measuring cellulases," "Measuring processivity" and "In situ cellulose detection with carbohydrate-binding modules."
1. Cover image 2. Title page 3. Table of Contents 4. Series Page 5. Copyright 6. Contributors 7. Preface 8. Methods in Enzymology 9. Chapter one: Measurement of endo-1,4-β-Glucanase 10. Chapter two: Biomass Conversion Determined via Fluorescent Cellulose Decay Assay 11. Chapter three: The Analysis of Saccharification in Biomass Using an Automated High-Throughput Method 12. Chapter four: Studies of Enzymatic Cleavage of Cellulose Using Polysaccharide Analysis by Carbohydrate gel Electrophoresis (PACE) 13. Chapter five: Measuring Processivity 14. Chapter six: Distinguishing Xyloglucanase Activity in endo-β(1 → 4)glucanases 15. Chapter seven: Methods for Structural Characterization of the Products of Cellulose- and Xyloglucan-Hydrolyzing Enzymes 16. Chapter eight: The Crystallization and Structural Analysis of Cellulases (and Other Glycoside Hydrolases) 17. Chapter nine: Visualization of Cellobiohydrolase I from Trichoderma reesei Moving on Crystalline Cellulose Using High-Speed Atomic Force Microscopy 18. Chapter ten: Small-Angle X-ray Scattering and Crystallography 19. Chapter eleven: Quantitative Approaches to The Analysis of Carbohydrate-Binding Module Function 20. Chapter twelve: In Situ Detection of Cellulose with Carbohydrate-Binding Modules 21. Chapter thirteen: Interactions Between Family 3 Carbohydrate Binding Modules (CBMs) and Cellulosomal Linker Peptides 22. Chapter fourteen: Approaches for Improving Thermostability Characteristics in Cellulases 23. Chapter fifteen: Thermophilic Glycosynthases for Oligosaccharides Synthesis 24. Chapter sixteen: Engineering Cellulase Activity into Clostridium acetobutylicum 25. Chapter seventeen: Transformation of Clostridium Thermocellum by Electroporation 26. Chapter eighteen: Genetic and Functional Genomic Approaches for the Study of Plant Cell Wall Degradation in Cellvibrio japonicus 27. Chapter nineteen: Methods for the Isolation of Cellulose-Degrading Microorganisms 28. Chapter twenty: Metagenomic Approaches to the Discovery of Cellulases 29. Chapter twenty-one: Escherichia coli Expression, Purification, Crystallization, and Structure Determination of Bacterial Cohesin–Dockerin Complexes 30. Chapter twenty-two: Measurements of Relative Binding of Cohesin and Dockerin Mutants Using an Advanced ELISA Technique for High-Affinity Interactions 31. Chapter twenty-three: Designer Cellulosomes for Enhanced Hydrolysis of Cellulosic Substrates 32. Chapter twenty-four: High-Throughput Screening of Cohesin Mutant Libraries on Cellulose Microarrays 33. Author Index 34. Subject Index
