Challenges in Delivery of Therapeutic Genomics and Proteomics, Second Edition is a complete reference on the biological principles involved in gene and protein delivery to cells and tissues. Highlighting the various chemical, physical, and biological approaches to protein and gene delivery, the book provides guidelines for pharmaceutical researchers in academia and corporate R&D. This new edition brings updates on the delivery of therapeutic proteomics and genomics in each chapter, and newly developed chapters on the regulatory aspects of related products, CRISPR/Cas9 gene editing, and computational tools in genomics and proteomics.
After an overview of the barriers to genomics and proteomics delivery, the book dives into physical, chemical, and biological methods of gene delivery. Further chapters extensively discuss the delivery of proteins and therapeutic peptides through the respiratory, oral, parenteral, transdermal, topical, uterine, and rectal pathways. This book is the ideal reference for pharmaceutical scientists dealing with gene and protein/peptide delivery. Regulators and corporate researchers can also benefit from the wide coverage of delivery methods presented.
1. 3.1 Introduction - p. 65 2. 3.2 Polymeric vectors - p. 66 3. 3.3 Lipidic vectors - p. 90 4. 3.4 Lipid polymer hybrid systems or lipopolymers - p. 105 5. 3.5 Peptides - p. 107 6. 3.6 Dendrimers - p. 110 7. 3.7 Biopolymers - p. 113 8. 3.8 Conclusion - p. 119 9. References - p. 120 10. Chapter 4 Gene delivery using viral vectors - p. 137 11. Chapter 5 RNA-based therapeutics—Mechanisms and challenges - p. 189 12. Chapter 6 Protein and peptide delivery through respiratory pathway - p. 227 13. Chapter 7 Oral delivery of proteins and peptides: Concepts and applications - p. 287 14. Chapter 8 Parenteral delivery of peptides and proteins - p. 345 15. Abstract - p. 345 16. 8.1 Introduction - p. 345 17. 8.2 Pharmacological considerations of P/P drugs - p. 347 18. 8.3 Techniques for stabilizing aqueous P/P formulation - p. 358 19. 8.4 Polymers used in parenteral delivery of P/P - p. 364 20. 8.5 Parenteral delivery systems for P/P - p. 368 21. 8.6 New advances in the parenteral administration of P/P - p. 394 22. 8.6.1 Pegylated proteins - p. 394 23. 8.6.2 Protein crystals - p. 400 24. 8.6.3 Prefilled syringes - p. 401 25. 8.6.4 Needle-free injections - p. 402 26. 8.6.5 Intracellular delivery of P/P by cell-penetrating peptides - p. 402 27. 8.6.6 Miscellaneous delivery system - p. 408 28. 8.7 Conclusion - p. 409 29. References - p. 410 30. Chapter 9 Other routes of protein and peptide delivery: Transdermal, topical, uterine, and rectal - p. 433 31. Chapter 10 Computational tools in genomics and proteomics - p. 489 32. Chapter 11 CRISPR/Cas9 gene editing for treating gene and protein disorders - p. 519 33. Chapter 12 Regulatory guidance on therapeutic proteomics and genomics - p. 555 34. Index - p. 587
Challenges in Delivery of Therapeutic Genomics and Proteomics, Second Edition is a complete reference on the biological principles involved in gene and protein delivery to cells and tissues. Highlighting the various chemical, physical, and biological approaches to protein and gene delivery, the book provides guidelines for pharmaceutical researchers in academia and corporate R&D. This new edition brings updates on the delivery of therapeutic proteomics and genomics in each chapter, and newly developed chapters on the regulatory aspects of related products, CRISPR/Cas9 gene editing, and computational tools in genomics and proteomics.
After an overview of the barriers to genomics and proteomics delivery, the book dives into physical, chemical, and biological methods of gene delivery. Further chapters extensively discuss the delivery of proteins and therapeutic peptides through the respiratory, oral, parenteral, transdermal, topical, uterine, and rectal pathways. This book is the ideal reference for pharmaceutical scientists dealing with gene and protein/peptide delivery. Regulators and corporate researchers can also benefit from the wide coverage of delivery methods presented.
1. 3.1 Introduction - p. 65 2. 3.2 Polymeric vectors - p. 66 3. 3.3 Lipidic vectors - p. 90 4. 3.4 Lipid polymer hybrid systems or lipopolymers - p. 105 5. 3.5 Peptides - p. 107 6. 3.6 Dendrimers - p. 110 7. 3.7 Biopolymers - p. 113 8. 3.8 Conclusion - p. 119 9. References - p. 120 10. Chapter 4 Gene delivery using viral vectors - p. 137 11. Chapter 5 RNA-based therapeutics—Mechanisms and challenges - p. 189 12. Chapter 6 Protein and peptide delivery through respiratory pathway - p. 227 13. Chapter 7 Oral delivery of proteins and peptides: Concepts and applications - p. 287 14. Chapter 8 Parenteral delivery of peptides and proteins - p. 345 15. Abstract - p. 345 16. 8.1 Introduction - p. 345 17. 8.2 Pharmacological considerations of P/P drugs - p. 347 18. 8.3 Techniques for stabilizing aqueous P/P formulation - p. 358 19. 8.4 Polymers used in parenteral delivery of P/P - p. 364 20. 8.5 Parenteral delivery systems for P/P - p. 368 21. 8.6 New advances in the parenteral administration of P/P - p. 394 22. 8.6.1 Pegylated proteins - p. 394 23. 8.6.2 Protein crystals - p. 400 24. 8.6.3 Prefilled syringes - p. 401 25. 8.6.4 Needle-free injections - p. 402 26. 8.6.5 Intracellular delivery of P/P by cell-penetrating peptides - p. 402 27. 8.6.6 Miscellaneous delivery system - p. 408 28. 8.7 Conclusion - p. 409 29. References - p. 410 30. Chapter 9 Other routes of protein and peptide delivery: Transdermal, topical, uterine, and rectal - p. 433 31. Chapter 10 Computational tools in genomics and proteomics - p. 489 32. Chapter 11 CRISPR/Cas9 gene editing for treating gene and protein disorders - p. 519 33. Chapter 12 Regulatory guidance on therapeutic proteomics and genomics - p. 555 34. Index - p. 587
