Chemical Microbiology, Part B, Volume 665, the latest release in the Methods of Enzymology series, highlights new advances in the field, including comprehensive chapters on the Application of Antibiotic-derived Fluorescent Probes to Bacterial Studies, Metabolomic approaches for enzyme function and pathway discovery in bacteria, Adding a diazo-transfer reagent to culture to generate secondary metabolite probes for click chemistry, Customized Peptidoglycan Surfaces to Investigate Innate Immune Recognition via Surface Plasmon Resonance, Development and application of highly sensitive labeling reagents for amino acids, Bacterial Cell Wall Modification with a Glycolipid Substrate, and much more.
1. Cover image - p. i 2. Table of Contents - p. iii 3. Series Page - p. iii 4. Copyright - p. iii 5. Contributors - p. iii 6. Preface - p. xv 7. Chapter One: Application of antibiotic-derived fluorescent probes to bacterial studies - p. 1 8. Chapter Two: Metabolomic approaches for enzyme function and pathway discovery in bacteria - p. 29 9. Chapter Three: Adding a diazo-transfer reagent to culture to generate secondary metabolite probes for click chemistry - p. 65 10. Chapter Four: Customized peptidoglycan surfaces to investigate innate immune recognition via surface plasmon resonance - p. 73 11. Chapter Five: Development and application of highly sensitive labeling reagents for amino acids - p. 105 12. Chapter Six: Biosynthetic incorporation for visualizing bacterial glycans - p. 135 13. Chapter Seven: Phenotypic screening of compound libraries as a platform for the identification of antibiotic adjuvants: Identification of colistin adjuvants from a natural product library - p. 153 14. Chapter Eight: Reactivity-based screening for natural product discovery - p. 177 15. Chapter Nine: Applications of an inactive Dispersin B probe to monitor biofilm polysaccharide production - p. 209 16. Chapter Ten: Synthesis and application of fluorescent teixobactin analogs - p. 233 17. Chapter Eleven: Impact of crossbridge structure on peptidoglycan crosslinking: A synthetic stem peptide approach - p. 259 18. Chapter Twelve: Utilizing imaging mass spectrometry to analyze microbial biofilm chemical responses to exogenous compounds - p. 281 19. Chapter Thirteen: Guidelines for metabolomics-guided transposon mutagenesis for microbial natural product discovery - p. 305 20. Chapter Fourteen: Glycopeptide antibiotic discovery in the genomic era - p. 325 21. Chapter Fifteen: Engineered biosynthesis of alkyne-tagged polyketides - p. 347
Chemical Microbiology, Part B, Volume 665, the latest release in the Methods of Enzymology series, highlights new advances in the field, including comprehensive chapters on the Application of Antibiotic-derived Fluorescent Probes to Bacterial Studies, Metabolomic approaches for enzyme function and pathway discovery in bacteria, Adding a diazo-transfer reagent to culture to generate secondary metabolite probes for click chemistry, Customized Peptidoglycan Surfaces to Investigate Innate Immune Recognition via Surface Plasmon Resonance, Development and application of highly sensitive labeling reagents for amino acids, Bacterial Cell Wall Modification with a Glycolipid Substrate, and much more.
1. Cover image - p. i 2. Table of Contents - p. iii 3. Series Page - p. iii 4. Copyright - p. iii 5. Contributors - p. iii 6. Preface - p. xv 7. Chapter One: Application of antibiotic-derived fluorescent probes to bacterial studies - p. 1 8. Chapter Two: Metabolomic approaches for enzyme function and pathway discovery in bacteria - p. 29 9. Chapter Three: Adding a diazo-transfer reagent to culture to generate secondary metabolite probes for click chemistry - p. 65 10. Chapter Four: Customized peptidoglycan surfaces to investigate innate immune recognition via surface plasmon resonance - p. 73 11. Chapter Five: Development and application of highly sensitive labeling reagents for amino acids - p. 105 12. Chapter Six: Biosynthetic incorporation for visualizing bacterial glycans - p. 135 13. Chapter Seven: Phenotypic screening of compound libraries as a platform for the identification of antibiotic adjuvants: Identification of colistin adjuvants from a natural product library - p. 153 14. Chapter Eight: Reactivity-based screening for natural product discovery - p. 177 15. Chapter Nine: Applications of an inactive Dispersin B probe to monitor biofilm polysaccharide production - p. 209 16. Chapter Ten: Synthesis and application of fluorescent teixobactin analogs - p. 233 17. Chapter Eleven: Impact of crossbridge structure on peptidoglycan crosslinking: A synthetic stem peptide approach - p. 259 18. Chapter Twelve: Utilizing imaging mass spectrometry to analyze microbial biofilm chemical responses to exogenous compounds - p. 281 19. Chapter Thirteen: Guidelines for metabolomics-guided transposon mutagenesis for microbial natural product discovery - p. 305 20. Chapter Fourteen: Glycopeptide antibiotic discovery in the genomic era - p. 325 21. Chapter Fifteen: Engineered biosynthesis of alkyne-tagged polyketides - p. 347
