Chromatin: Structure, Function, and History covers the basics of chromatin biology, beginning with the discoveries that culminated in the recognition of the nucleosome as the basic subunit of chromatin. Chromatin folding, nucleosome positioning, and histone variants are discussed, as well as research on chromatin modifications and remodeling, which exploded in the early to mid-1990s and led to widespread interest in epigenetics. Considerable attention is given to methods and experiments that led to key insights and recent developments such as the use of genome-wide approaches and innovations in imaging approaches are also emphasized.
By providing historical background together with detailed discussion of contemporary studies, the book aims to instill in the reader an appreciation not only of our current knowledge of chromatin structure and function, but also of the remarkable path that has taken chromatin to the forefront of modern research.
1. Title of Book - p. Cover 2. Cover image - p. Cover 3. Title page - p. ii 4. Table of Contents - p. iii 5. Copyright - p. iii 6. Preface - p. vii 7. Part 1: Chromatin structure - p. viii 8. Chapter 1. Early history - p. 3 9. Chapter 2. The nucleosome unveiled - p. 23 10. Chapter 3. Chromatin compaction - p. 49 11. Chapter 4. Nucleosomes in context: Positioning, occupancy, and spacing - p. 140 12. Chapter 5. Histones and variants - p. 189 13. Part 2: Chromatin function - p. 279 14. Chapter 6. Chromatin remodeling - p. 283 15. Chapter 7. Histone modifications - p. 355 16. Abstract - p. 355 17. Overview - p. 355 18. Histone acetylation and deacetylation - p. 336 19. Histone methylation and demethylation - p. 372 20. Other modifications - p. 391 21. Cross-talk - p. 400 22. The histone code - p. 403 23. References - p. 406 24. Chapter 8. Chromatin and transcription - p. 443 25. Abstract - p. 443 26. Abbreviations - p. 444 27. Transcription: A brief review - p. 444 28. Early investigations - p. 446 29. Prokaryotic polymerases and RNA polymerase III - p. 447 30. Pol II - p. 452 31. Transcription factor access in a chromatin milieu - p. 472 32. Transcribing through chromatin - p. 501 33. Epigenetics - p. 518 34. References - p. 531 35. Epilogue - p. 563 36. Index - p. 567
Chromatin: Structure, Function, and History covers the basics of chromatin biology, beginning with the discoveries that culminated in the recognition of the nucleosome as the basic subunit of chromatin. Chromatin folding, nucleosome positioning, and histone variants are discussed, as well as research on chromatin modifications and remodeling, which exploded in the early to mid-1990s and led to widespread interest in epigenetics. Considerable attention is given to methods and experiments that led to key insights and recent developments such as the use of genome-wide approaches and innovations in imaging approaches are also emphasized.
By providing historical background together with detailed discussion of contemporary studies, the book aims to instill in the reader an appreciation not only of our current knowledge of chromatin structure and function, but also of the remarkable path that has taken chromatin to the forefront of modern research.
1. Title of Book - p. Cover 2. Cover image - p. Cover 3. Title page - p. ii 4. Table of Contents - p. iii 5. Copyright - p. iii 6. Preface - p. vii 7. Part 1: Chromatin structure - p. viii 8. Chapter 1. Early history - p. 3 9. Chapter 2. The nucleosome unveiled - p. 23 10. Chapter 3. Chromatin compaction - p. 49 11. Chapter 4. Nucleosomes in context: Positioning, occupancy, and spacing - p. 140 12. Chapter 5. Histones and variants - p. 189 13. Part 2: Chromatin function - p. 279 14. Chapter 6. Chromatin remodeling - p. 283 15. Chapter 7. Histone modifications - p. 355 16. Abstract - p. 355 17. Overview - p. 355 18. Histone acetylation and deacetylation - p. 336 19. Histone methylation and demethylation - p. 372 20. Other modifications - p. 391 21. Cross-talk - p. 400 22. The histone code - p. 403 23. References - p. 406 24. Chapter 8. Chromatin and transcription - p. 443 25. Abstract - p. 443 26. Abbreviations - p. 444 27. Transcription: A brief review - p. 444 28. Early investigations - p. 446 29. Prokaryotic polymerases and RNA polymerase III - p. 447 30. Pol II - p. 452 31. Transcription factor access in a chromatin milieu - p. 472 32. Transcribing through chromatin - p. 501 33. Epigenetics - p. 518 34. References - p. 531 35. Epilogue - p. 563 36. Index - p. 567
