Concepts and Models for Drug Permeability Studies: Cell and Tissue Based in Vitro Culture Models, Second Edition, summarizes the most important developments in in vitro models for predicting the permeability of drugs. This book is structured around three different approaches, summarizing the most recent achievements regarding models comprising (i) immortalized cells with an intrinsic ability to grow as monolayers when seeded in permeable supports, (ii) primary cells isolated from living organisms and directly cultured as barrier monolayers, and (iii) tissue-based models constructed with cell lines and extracellular matrix that resembles the tridimensional structure of mucosae and other biological membranes, or animal/patient-derived tissues. Each model is covered in detail, including the protocol of generation and application for specific drugs/drug delivery systems. The equivalence between in vitro cell and tissue models and in vivo conditions is discussed, highlighting how each model may provisionally resemble different drug absorption route. Chapters included in the first edition were updated with relevant data published in recent years, while four new chapters were included to reflect new emerging directions and trends in drug permeability models.
Concepts and Models for Drug Permeability Studies: Cell and Tissue Based in Vitro Culture Models, Second Edition, is a critical reference for drug discovery and drug formulation scientists interested in delivery systems intended for the administration of drugs through mucosal routes and other important tissue barriers (e.g. the BBB). Researchers studying mucosal biology can use this book to familiarize themselves and exploit the synergic effect of mucosal delivery systems and biomolecules.
1. Chapter 1 Introduction - p. 1 2. Chapter 2 Importance and applications of cell- and tissue-based in vitro models for drug permeability screening in early stages of drug development - p. 5 3. Chapter 3.1 Cell-based in vitro models for buccal permeability studies - p. 45 4. Chapter 3.2 Cell-based in vitro models for gastric permeability studies - p. 67 5. Chapter 3.3 Cell-based in vitro models for intestinal permeability studies - p. 89 6. Chapter 3.4 Cell-based in vitro models for nasal permeability studies - p. 109 7. Chapter 3.5 Cell-based in vitro models for pulmonary permeability studies - p. 137 8. Chapter 3.6 Cell-based in vitro models for vaginal permeability studies - p. 169 9. Chapter 3.7 Cell-based in vitro models for transcorneal permeability studies - p. 187 10. Chapter 3.8 Cell-based in vitro models for dermal permeability studies - p. 219 11. Chapter 3.9 Tissue-based in vitro and ex vivo models for blood–brain barrier permeability studies - p. 233 12. Chapter 3.10 Cell-based in vitro models for placental barrier permeability studies - p. 251 13. Chapter 4.1 Tissue-based in-vitro models for buccal permeability studies - p. 275 14. Chapter 4.2 Tissue-based in vitro and ex vivo models for intestinal permeability studies - p. 309 15. Chapter 4.3 Tissue-based in vitro and ex vivo models for nasal permeability studies - p. 347 16. Chapter 4.4 Tissue-based in vitro and ex vivo models for pulmonary permeability studies - p. 373 17. Chapter 4.5 Tissue-based models for vaginal permeability studies - p. 401 18. Chapter 4.6 Tissue-based models for ocular permeability studies - p. 451 19. Chapter 4.7 Tissue-based in vitro and ex vivo models for dermal permeability studies - p. 479 20. Chapter 4.8 Tissue-based in vitro and ex vivo models for blood–brain barrier permeability studies - p. 501 21. Chapter 4.9 Tissue-based ex vivo models for placental barrier permeability studies - p. 515 22. Chapter 5.1 Organ-on-chip models for intestinal permeability studies - p. 537 23. Chapter 5.2 Organ-on-chip models for pulmonary permeability studies - p. 563 24. Chapter 5.3 Organ-on-chip models for blood–brain barrier permeability studies - p. 577 25. Chapter 5.4 Organ-on-chip models for other mucosal permeability studies - p. 593 26. Chapter 6.1 Multicompartmental dynamic models for permeability studies - p. 613 27. Chapter 6.2 Organoid-based models for permeability studies - p. 627 28. Chapter 7 Correlation between cell- and tissue-based in vitro models for drug permeability screening with in vivo situation: modeling and functional extrapolation - p. 641 29. Index - p. 665
Concepts and Models for Drug Permeability Studies: Cell and Tissue Based in Vitro Culture Models, Second Edition, summarizes the most important developments in in vitro models for predicting the permeability of drugs. This book is structured around three different approaches, summarizing the most recent achievements regarding models comprising (i) immortalized cells with an intrinsic ability to grow as monolayers when seeded in permeable supports, (ii) primary cells isolated from living organisms and directly cultured as barrier monolayers, and (iii) tissue-based models constructed with cell lines and extracellular matrix that resembles the tridimensional structure of mucosae and other biological membranes, or animal/patient-derived tissues. Each model is covered in detail, including the protocol of generation and application for specific drugs/drug delivery systems. The equivalence between in vitro cell and tissue models and in vivo conditions is discussed, highlighting how each model may provisionally resemble different drug absorption route. Chapters included in the first edition were updated with relevant data published in recent years, while four new chapters were included to reflect new emerging directions and trends in drug permeability models.
Concepts and Models for Drug Permeability Studies: Cell and Tissue Based in Vitro Culture Models, Second Edition, is a critical reference for drug discovery and drug formulation scientists interested in delivery systems intended for the administration of drugs through mucosal routes and other important tissue barriers (e.g. the BBB). Researchers studying mucosal biology can use this book to familiarize themselves and exploit the synergic effect of mucosal delivery systems and biomolecules.
1. Chapter 1 Introduction - p. 1 2. Chapter 2 Importance and applications of cell- and tissue-based in vitro models for drug permeability screening in early stages of drug development - p. 5 3. Chapter 3.1 Cell-based in vitro models for buccal permeability studies - p. 45 4. Chapter 3.2 Cell-based in vitro models for gastric permeability studies - p. 67 5. Chapter 3.3 Cell-based in vitro models for intestinal permeability studies - p. 89 6. Chapter 3.4 Cell-based in vitro models for nasal permeability studies - p. 109 7. Chapter 3.5 Cell-based in vitro models for pulmonary permeability studies - p. 137 8. Chapter 3.6 Cell-based in vitro models for vaginal permeability studies - p. 169 9. Chapter 3.7 Cell-based in vitro models for transcorneal permeability studies - p. 187 10. Chapter 3.8 Cell-based in vitro models for dermal permeability studies - p. 219 11. Chapter 3.9 Tissue-based in vitro and ex vivo models for blood–brain barrier permeability studies - p. 233 12. Chapter 3.10 Cell-based in vitro models for placental barrier permeability studies - p. 251 13. Chapter 4.1 Tissue-based in-vitro models for buccal permeability studies - p. 275 14. Chapter 4.2 Tissue-based in vitro and ex vivo models for intestinal permeability studies - p. 309 15. Chapter 4.3 Tissue-based in vitro and ex vivo models for nasal permeability studies - p. 347 16. Chapter 4.4 Tissue-based in vitro and ex vivo models for pulmonary permeability studies - p. 373 17. Chapter 4.5 Tissue-based models for vaginal permeability studies - p. 401 18. Chapter 4.6 Tissue-based models for ocular permeability studies - p. 451 19. Chapter 4.7 Tissue-based in vitro and ex vivo models for dermal permeability studies - p. 479 20. Chapter 4.8 Tissue-based in vitro and ex vivo models for blood–brain barrier permeability studies - p. 501 21. Chapter 4.9 Tissue-based ex vivo models for placental barrier permeability studies - p. 515 22. Chapter 5.1 Organ-on-chip models for intestinal permeability studies - p. 537 23. Chapter 5.2 Organ-on-chip models for pulmonary permeability studies - p. 563 24. Chapter 5.3 Organ-on-chip models for blood–brain barrier permeability studies - p. 577 25. Chapter 5.4 Organ-on-chip models for other mucosal permeability studies - p. 593 26. Chapter 6.1 Multicompartmental dynamic models for permeability studies - p. 613 27. Chapter 6.2 Organoid-based models for permeability studies - p. 627 28. Chapter 7 Correlation between cell- and tissue-based in vitro models for drug permeability screening with in vivo situation: modeling and functional extrapolation - p. 641 29. Index - p. 665
