Kinetic studies of enzyme action provide powerful insights into the underlying mechanisms of catalysis and regulation. These approaches are equally useful in examining the action of newly discovered enzymes and therapeutic agents.
Contemporary Enzyme Kinetics and Mechanism, Second Edition presents key articles from Volumes 63, 64, 87, 249, 308 and 354 of Methods in Enzymology. The chapters describe the most essential and widely applied strategies. A set of exercises and problems is included to facilitate mastery of these topics.
The book will aid the reader to design, execute, and analyze kinetic experiments on enzymes. Its emphasis on enzyme inhibition will also make it attractive to pharmacologists and pharmaceutical chemists interested in rational drug design.
Of the seventeen chapters presented in this new edition, ten did not previously appear in the first edition.
1. Cover 2. Title Page 3. Copyright 4. Table of Contents 5. Preface 6. Contributors 7. Chapter 1: Derivation of Initial-Velocity and Isotope-Exchange Rate Equations 8. Chapter 2: Practical Considerations in the Design of Initial Velocity Enzyme Rate Assays 9. Chapter 3: Techniques in Coupled Enzyme Assays 10. Chapter 4: Regression Analysis, Experimental Error, and Statistical Criteria in the Design and Analysis of Experiments for Discrimination Between Rival Kinetic Models 11. Chapter 5: Analysis of Enzyme Progress Curves by Nonlinear Regression 12. Chapter 6: Effects of pH on Enzymes 13. Chapter 7: Temperature Effects in Enzyme Kinetics 14. Chapter 8: Site-Directed Mutagenesis 15. Chapter 9: Cooperativity in Enzyme Function 16. Chapter 10: Reversible Enzyme Inhibitors as Mechanistic Probes 17. Chapter 11: Application of Affinity Labeling for Studying Structure and Function of Enzymes 18. Chapter 12: Mechanism-Based Enzyme Inactivators 19. Chapter 13: Transient Kinetic Approaches to Enzyme Mechanisms 20. Chapter 14: Rapid Quench Kinetic Analysis of Polymerases, Adenosinetriphosphatases, and Enzyme Intermediates 21. Chapter 15: Presteady-State Kinetics of Enzymatic Reactions Studied by Electrospray Mass Spectrometry with Online Rapid-Mixing Techniques 22. Chapter 16: Isotope Exchange Methods for Elucidating Enzymic Catalysis 23. Chapter 17: Positional Isotope Exchange as Probe of Enzyme Action 24. Chapter 18: Enzymatic Transition-State Analysis and Transition-State Analogs 25. Chapter 19: Determining Transition States from Kinetic Isotope Effects 26. Chapter 20: Computational Methods for Transition State and Inhibitor Recognition 27. Appendix: Selected Exercises and Problems 28. Index
Kinetic studies of enzyme action provide powerful insights into the underlying mechanisms of catalysis and regulation. These approaches are equally useful in examining the action of newly discovered enzymes and therapeutic agents.
Contemporary Enzyme Kinetics and Mechanism, Second Edition presents key articles from Volumes 63, 64, 87, 249, 308 and 354 of Methods in Enzymology. The chapters describe the most essential and widely applied strategies. A set of exercises and problems is included to facilitate mastery of these topics.
The book will aid the reader to design, execute, and analyze kinetic experiments on enzymes. Its emphasis on enzyme inhibition will also make it attractive to pharmacologists and pharmaceutical chemists interested in rational drug design.
Of the seventeen chapters presented in this new edition, ten did not previously appear in the first edition.
1. Cover 2. Title Page 3. Copyright 4. Table of Contents 5. Preface 6. Contributors 7. Chapter 1: Derivation of Initial-Velocity and Isotope-Exchange Rate Equations 8. Chapter 2: Practical Considerations in the Design of Initial Velocity Enzyme Rate Assays 9. Chapter 3: Techniques in Coupled Enzyme Assays 10. Chapter 4: Regression Analysis, Experimental Error, and Statistical Criteria in the Design and Analysis of Experiments for Discrimination Between Rival Kinetic Models 11. Chapter 5: Analysis of Enzyme Progress Curves by Nonlinear Regression 12. Chapter 6: Effects of pH on Enzymes 13. Chapter 7: Temperature Effects in Enzyme Kinetics 14. Chapter 8: Site-Directed Mutagenesis 15. Chapter 9: Cooperativity in Enzyme Function 16. Chapter 10: Reversible Enzyme Inhibitors as Mechanistic Probes 17. Chapter 11: Application of Affinity Labeling for Studying Structure and Function of Enzymes 18. Chapter 12: Mechanism-Based Enzyme Inactivators 19. Chapter 13: Transient Kinetic Approaches to Enzyme Mechanisms 20. Chapter 14: Rapid Quench Kinetic Analysis of Polymerases, Adenosinetriphosphatases, and Enzyme Intermediates 21. Chapter 15: Presteady-State Kinetics of Enzymatic Reactions Studied by Electrospray Mass Spectrometry with Online Rapid-Mixing Techniques 22. Chapter 16: Isotope Exchange Methods for Elucidating Enzymic Catalysis 23. Chapter 17: Positional Isotope Exchange as Probe of Enzyme Action 24. Chapter 18: Enzymatic Transition-State Analysis and Transition-State Analogs 25. Chapter 19: Determining Transition States from Kinetic Isotope Effects 26. Chapter 20: Computational Methods for Transition State and Inhibitor Recognition 27. Appendix: Selected Exercises and Problems 28. Index
