The combination of electron microscopy with transmitted light microscopy (termed correlative light and electron microscopy; CLEM) has been employed for decades to generate molecular identification that can be visualized by a dark, electron-dense precipitate. This new volume of Methods in Cell Biology covers many areas of CLEM, including a brief history and overview on CLEM methods, imaging of intermediate stages of meiotic spindle assembly in C. elegans embryos using CLEM, and capturing endocytic segregation events with HPF-CLEM.
1. Cover image 2. Title page 3. Table of Contents 4. Series Editors 5. Front Matter 6. Copyright 7. Contributors 8. Preface 9. Chapter 1 Imaging Fluorescently Labeled Complexes by Means of Multidimensional Correlative Light and Transmission Electron Microscopy 10. Chapter 2 Visualizing Live Dynamics and Ultrastructure of Intracellular Organelles with Preembedding Correlative Light-Electron Microscopy 11. Chapter 3 Correlative Fluorescence and Transmission Electron Microscopy in Tissues 12. Chapter 4 Correlative Light and Electron Microscopy in Parasite Research 13. Chapter 5 Labeling of Ultrathin Resin Sections for Correlative Light and Electron Microscopy 14. Chapter 6 3D HDO-CLEM 15. Chapter 7 Correlative Light and Electron Microscopy of GFP 16. Chapter 8 Picking Faces out of a Crowd 17. Chapter 9 Correlated Light Microscopy and Electron Microscopy 18. Chapter 10 Capturing Endocytic Segregation Events with HPF-CLEM 19. Chapter 11 Targeted Ultramicrotomy 20. Chapter 12 Correlative Light and Electron Microscopy of Intermediate Stages of Meiotic Spindle Assembly in the Early Caenorhabditis elegans Embryo 21. Chapter 13 Precise, Correlated Fluorescence Microscopy and Electron Tomography of Lowicryl Sections Using Fluorescent Fiducial Markers 22. Chapter 14 Integrative Approaches for Cellular Cryo-electron Tomography 23. Chapter 15 Visualizing Proteins in Electron Micrographs at Nanometer Resolution 24. Chapter 16 Atmospheric Scanning Electron Microscope for Correlative Microscopy 25. Chapter 17 Bridging Microscopes 26. Chapter 18 Correlative Light and Volume Electron Microscopy 27. Index 28. Volumes in Series 29. Colour Plate
The combination of electron microscopy with transmitted light microscopy (termed correlative light and electron microscopy; CLEM) has been employed for decades to generate molecular identification that can be visualized by a dark, electron-dense precipitate. This new volume of Methods in Cell Biology covers many areas of CLEM, including a brief history and overview on CLEM methods, imaging of intermediate stages of meiotic spindle assembly in C. elegans embryos using CLEM, and capturing endocytic segregation events with HPF-CLEM.
1. Cover image 2. Title page 3. Table of Contents 4. Series Editors 5. Front Matter 6. Copyright 7. Contributors 8. Preface 9. Chapter 1 Imaging Fluorescently Labeled Complexes by Means of Multidimensional Correlative Light and Transmission Electron Microscopy 10. Chapter 2 Visualizing Live Dynamics and Ultrastructure of Intracellular Organelles with Preembedding Correlative Light-Electron Microscopy 11. Chapter 3 Correlative Fluorescence and Transmission Electron Microscopy in Tissues 12. Chapter 4 Correlative Light and Electron Microscopy in Parasite Research 13. Chapter 5 Labeling of Ultrathin Resin Sections for Correlative Light and Electron Microscopy 14. Chapter 6 3D HDO-CLEM 15. Chapter 7 Correlative Light and Electron Microscopy of GFP 16. Chapter 8 Picking Faces out of a Crowd 17. Chapter 9 Correlated Light Microscopy and Electron Microscopy 18. Chapter 10 Capturing Endocytic Segregation Events with HPF-CLEM 19. Chapter 11 Targeted Ultramicrotomy 20. Chapter 12 Correlative Light and Electron Microscopy of Intermediate Stages of Meiotic Spindle Assembly in the Early Caenorhabditis elegans Embryo 21. Chapter 13 Precise, Correlated Fluorescence Microscopy and Electron Tomography of Lowicryl Sections Using Fluorescent Fiducial Markers 22. Chapter 14 Integrative Approaches for Cellular Cryo-electron Tomography 23. Chapter 15 Visualizing Proteins in Electron Micrographs at Nanometer Resolution 24. Chapter 16 Atmospheric Scanning Electron Microscope for Correlative Microscopy 25. Chapter 17 Bridging Microscopes 26. Chapter 18 Correlative Light and Volume Electron Microscopy 27. Index 28. Volumes in Series 29. Colour Plate
