Plants are vulnerable to pathogens including fungi, bacteria, and viruses, which cause critical problems and deficits. Crop protection by plant breeding delivers a promising solution with no obvious effect on human health or the local ecosystem. Crop improvement has been the most powerful approach for producing unique crop cultivars since domestication occurred, making possible the main innovations in feeding the globe and community development. Genome editing is one of the genetic devices that can be implemented, and disease resistance is frequently cited as the most encouraging application of CRISPR/Cas9 technology in agriculture. Nanobiotechnology has harnessed the power of genome editing to develop agricultural crops. Nanosized DNA or RNA nanotechnology approaches could contribute to raising the stability and performance of CRISPR guide RNAs. This book brings together the latest research in these areas.
CRISPR and RNAi Systems: Nanobiotechnology Approaches to Plant Breeding and Protection presents a complete understanding of the RNAi and CRISPR/Cas9 techniques for controlling mycotoxins, fighting plant nematodes, and detecting plant pathogens. CRISPR/Cas genome editing enables efficient targeted modification in most crops, thus promising to accelerate crop improvement. CRISPR/Cas9 can be used for management of plant insects, and various plant pathogens. The book is an important reference source for both plant scientists and environmental scientists who want to understand how nano biotechnologically based approaches are being used to create more efficient plant protection and plant breeding systems.
1. CRISPR and RNAi Systems - p. Page i 2. Cover - p. Page i 3. Title page - p. Page ii 4. Table of Contents - p. Page iii 5. Copyright - p. Page iii 6. Contents - p. Page vi 7. List of contributors - p. Page vi 8. Series preface - p. Page xiv 9. Preface - p. Page xvi 10. List of Illustrations - p. Page xix 11. List of Tables - p. Page xx 12. Chapter 1 : Can CRISPRized crops save the global food supply? - p. Page xx 13. Chapter 2 : Targeted genome engineering for insects control - p. Page 12 14. Chapter 3 : CRISPR/Cas9 regulations in plant science - p. Page 27 15. Chapter 4 : Are CRISPR/Cas9 and RNA interference-based new technologies to relocate crop pesticides? - p. Page 43 16. Chapter 5 : CRISPR-Cas epigenome editing: improving crop resistance to pathogens - p. Page 57 17. Chapter 6 : CRISPR/Cas system for the development of disease resistance in horticulture crops - p. Page 97 18. Chapter 7 : CRISPR and RNAi technology for crop improvements in the developing countries - p. Page 123 19. Chapter 8 : RNA interference and CRISPR/Cas9 applications for virus resistance - p. Page 149 20. Chapter 9 : Current trends and recent progress of genetic engineering in genus Phytophthora using CRISPR systems - p. Page 178 21. Chapter 10 : CRISPR/Cas9 and Cas13a systems: a promising tool for plant breeding and plant defence - p. Page 202 22. Chapter 11 : CRISPR/Cas techniques: a new method for RNA interference in cereals - p. Page 225 23. Chapter 12 : Genetic transformation methods and advancement of CRISPR/Cas9 technology in wheat - p. Page 246 24. Chapter 13 : Application of CRISPR/Cas system for genome editing in cotton - p. Page 268 25. Chapter 14 : Resistant starch: biosynthesis, regulatory pathways, and engineering via CRISPR system - p. Page 293 26. Chapter 15 : Role of CRISPR/Cas system in altering phenolic and carotenoid biosynthesis in plants defense activation - p. Page 312 27. Chapter 16 : Fungal genome editing using CRISPR-Cas nucleases: a new tool for the management of plant diseases - p. Page 329 28. Chapter 17 : CRISPR–Cas systems as antimicrobial agents for agri-food pathogens - p. Page 351 29. Chapter 18 : CRISPR interference system: a potential strategy to inhibit pathogenic biofilm in the agri-food sector - p. Page 379 30. Chapter 19 : Patenting dynamics in CRISPR gene editing technologies - p. Page 400 31. Chapter 20 : Tricks and trends in CRISPR/Cas9-based genome editing and use of bioinformatics tools for improving on-target efficiency - p. Page 437 32. Chapter 21 : RNA interference and CRISPR/Cas9 techniques for controlling mycotoxins - p. Page 458 33. Chapter 22 : Role of small RNA and RNAi technology toward improvement of abiotic stress tolerance in plants - p. Page 483 34. Chapter 23 : RNAi-based system a new tool for insects’ control - p. Page 502 35. Chapter 24 : RNAi strategy for management of phytopathogenic fungi - p. Page 525 36. Chapter 25 : CRISPR applications in plant bacteriology: today and future perspectives - p. Page 545 37. Chapter 26 : RNAi-based gene silencing in plant-parasitic nematodes: a road toward crop improvements - p. Page 571 38. Chapter 27 : RNA interference-mediated viral disease resistance in crop plants - p. Page 591 39. Chapter 28 : Phytoalexin biosynthesis through RNA interference for disease resistance in plants - p. Page 612 40. Chapter 29 : Polymer and lipid-based nanoparticles to deliver RNAi and CRISPR systems - p. Page 630 41. Chapter 30 : Inorganic smart nanoparticles: a new tool to deliver CRISPR systems into plant cells - p. Page 654 42. Chapter 31 : Regulatory aspects, risk assessment, and toxicity associated with RNAi and CRISPR methods - p. Page 679 43. Chapter 32 : Gene editing in filamentous fungi and oomycetes using CRISPR-Cas technology - p. Page 715 44. Chapter 33 : CRISPR–Cas technology towards improvement of abiotic stress tolerance in plants - p. Page 747 45. Chapter 34 : Databases and bioinformatics tools for genome engineering in plants using RNA interference - p. Page 768 46. Index - p. Page 783
Plants are vulnerable to pathogens including fungi, bacteria, and viruses, which cause critical problems and deficits. Crop protection by plant breeding delivers a promising solution with no obvious effect on human health or the local ecosystem. Crop improvement has been the most powerful approach for producing unique crop cultivars since domestication occurred, making possible the main innovations in feeding the globe and community development. Genome editing is one of the genetic devices that can be implemented, and disease resistance is frequently cited as the most encouraging application of CRISPR/Cas9 technology in agriculture. Nanobiotechnology has harnessed the power of genome editing to develop agricultural crops. Nanosized DNA or RNA nanotechnology approaches could contribute to raising the stability and performance of CRISPR guide RNAs. This book brings together the latest research in these areas.
CRISPR and RNAi Systems: Nanobiotechnology Approaches to Plant Breeding and Protection presents a complete understanding of the RNAi and CRISPR/Cas9 techniques for controlling mycotoxins, fighting plant nematodes, and detecting plant pathogens. CRISPR/Cas genome editing enables efficient targeted modification in most crops, thus promising to accelerate crop improvement. CRISPR/Cas9 can be used for management of plant insects, and various plant pathogens. The book is an important reference source for both plant scientists and environmental scientists who want to understand how nano biotechnologically based approaches are being used to create more efficient plant protection and plant breeding systems.
1. CRISPR and RNAi Systems - p. Page i 2. Cover - p. Page i 3. Title page - p. Page ii 4. Table of Contents - p. Page iii 5. Copyright - p. Page iii 6. Contents - p. Page vi 7. List of contributors - p. Page vi 8. Series preface - p. Page xiv 9. Preface - p. Page xvi 10. List of Illustrations - p. Page xix 11. List of Tables - p. Page xx 12. Chapter 1 : Can CRISPRized crops save the global food supply? - p. Page xx 13. Chapter 2 : Targeted genome engineering for insects control - p. Page 12 14. Chapter 3 : CRISPR/Cas9 regulations in plant science - p. Page 27 15. Chapter 4 : Are CRISPR/Cas9 and RNA interference-based new technologies to relocate crop pesticides? - p. Page 43 16. Chapter 5 : CRISPR-Cas epigenome editing: improving crop resistance to pathogens - p. Page 57 17. Chapter 6 : CRISPR/Cas system for the development of disease resistance in horticulture crops - p. Page 97 18. Chapter 7 : CRISPR and RNAi technology for crop improvements in the developing countries - p. Page 123 19. Chapter 8 : RNA interference and CRISPR/Cas9 applications for virus resistance - p. Page 149 20. Chapter 9 : Current trends and recent progress of genetic engineering in genus Phytophthora using CRISPR systems - p. Page 178 21. Chapter 10 : CRISPR/Cas9 and Cas13a systems: a promising tool for plant breeding and plant defence - p. Page 202 22. Chapter 11 : CRISPR/Cas techniques: a new method for RNA interference in cereals - p. Page 225 23. Chapter 12 : Genetic transformation methods and advancement of CRISPR/Cas9 technology in wheat - p. Page 246 24. Chapter 13 : Application of CRISPR/Cas system for genome editing in cotton - p. Page 268 25. Chapter 14 : Resistant starch: biosynthesis, regulatory pathways, and engineering via CRISPR system - p. Page 293 26. Chapter 15 : Role of CRISPR/Cas system in altering phenolic and carotenoid biosynthesis in plants defense activation - p. Page 312 27. Chapter 16 : Fungal genome editing using CRISPR-Cas nucleases: a new tool for the management of plant diseases - p. Page 329 28. Chapter 17 : CRISPR–Cas systems as antimicrobial agents for agri-food pathogens - p. Page 351 29. Chapter 18 : CRISPR interference system: a potential strategy to inhibit pathogenic biofilm in the agri-food sector - p. Page 379 30. Chapter 19 : Patenting dynamics in CRISPR gene editing technologies - p. Page 400 31. Chapter 20 : Tricks and trends in CRISPR/Cas9-based genome editing and use of bioinformatics tools for improving on-target efficiency - p. Page 437 32. Chapter 21 : RNA interference and CRISPR/Cas9 techniques for controlling mycotoxins - p. Page 458 33. Chapter 22 : Role of small RNA and RNAi technology toward improvement of abiotic stress tolerance in plants - p. Page 483 34. Chapter 23 : RNAi-based system a new tool for insects’ control - p. Page 502 35. Chapter 24 : RNAi strategy for management of phytopathogenic fungi - p. Page 525 36. Chapter 25 : CRISPR applications in plant bacteriology: today and future perspectives - p. Page 545 37. Chapter 26 : RNAi-based gene silencing in plant-parasitic nematodes: a road toward crop improvements - p. Page 571 38. Chapter 27 : RNA interference-mediated viral disease resistance in crop plants - p. Page 591 39. Chapter 28 : Phytoalexin biosynthesis through RNA interference for disease resistance in plants - p. Page 612 40. Chapter 29 : Polymer and lipid-based nanoparticles to deliver RNAi and CRISPR systems - p. Page 630 41. Chapter 30 : Inorganic smart nanoparticles: a new tool to deliver CRISPR systems into plant cells - p. Page 654 42. Chapter 31 : Regulatory aspects, risk assessment, and toxicity associated with RNAi and CRISPR methods - p. Page 679 43. Chapter 32 : Gene editing in filamentous fungi and oomycetes using CRISPR-Cas technology - p. Page 715 44. Chapter 33 : CRISPR–Cas technology towards improvement of abiotic stress tolerance in plants - p. Page 747 45. Chapter 34 : Databases and bioinformatics tools for genome engineering in plants using RNA interference - p. Page 768 46. Index - p. Page 783
