CRISPR Genome Surgery in Stem Cells and Disease Tissues focuses uniquely on the clinical applications of CRISPR/Cas9 based technology. Topics include the latest advances in gene editing and its translational applications to various diseases, including retinal degenerative disease, recessively inherited diseases, and dominantly inherited diseases, to name a few. The book's target audience includes researchers, students, clinicians and the general public. This space that is not currently served by any existing resource, so this publication fills a gap in current literature.
1. Title of Book - p. i 2. Cover image - p. i 3. Title page - p. ii 4. Table of Contents - p. iii 5. Copyright - p. iv 6. List of contributors - p. vii 7. Foreword - p. ix 8. Chapter 1. The history of CRISPR: from discovery to the present - p. 1 9. Chapter 2. CRISPR-Cas orthologs and variants - p. 7 10. Chapter 3. Genome engineering with CRISPR/Cas9, ZFNs, and TALENs - p. 39 11. Chapter 4. Single-guide RNAs: rationale and design - p. 47 12. Chapter 5. CRISPR-mediated dense mutagenesis: a tool for rational targeting of multiprotein complexes and the noncoding genome - p. 57 13. Chapter 6. Targeting Alzheimer’s disease and related dementias with CRISPR and human pluripotent stem cell technologies - p. 65 14. Chapter 7. Gene editing for the cornea - p. 81 15. Chapter 8. Employing nonhomologous end joining and homology-directed repair for treatment of Leber congenital amaurosis and inherited retinal degeneration - p. 101 16. Chapter 9. The ethics of gene editing in human stem cells - p. 111 17. Index - p. 123
CRISPR Genome Surgery in Stem Cells and Disease Tissues focuses uniquely on the clinical applications of CRISPR/Cas9 based technology. Topics include the latest advances in gene editing and its translational applications to various diseases, including retinal degenerative disease, recessively inherited diseases, and dominantly inherited diseases, to name a few. The book's target audience includes researchers, students, clinicians and the general public. This space that is not currently served by any existing resource, so this publication fills a gap in current literature.
1. Title of Book - p. i 2. Cover image - p. i 3. Title page - p. ii 4. Table of Contents - p. iii 5. Copyright - p. iv 6. List of contributors - p. vii 7. Foreword - p. ix 8. Chapter 1. The history of CRISPR: from discovery to the present - p. 1 9. Chapter 2. CRISPR-Cas orthologs and variants - p. 7 10. Chapter 3. Genome engineering with CRISPR/Cas9, ZFNs, and TALENs - p. 39 11. Chapter 4. Single-guide RNAs: rationale and design - p. 47 12. Chapter 5. CRISPR-mediated dense mutagenesis: a tool for rational targeting of multiprotein complexes and the noncoding genome - p. 57 13. Chapter 6. Targeting Alzheimer’s disease and related dementias with CRISPR and human pluripotent stem cell technologies - p. 65 14. Chapter 7. Gene editing for the cornea - p. 81 15. Chapter 8. Employing nonhomologous end joining and homology-directed repair for treatment of Leber congenital amaurosis and inherited retinal degeneration - p. 101 16. Chapter 9. The ethics of gene editing in human stem cells - p. 111 17. Index - p. 123
