Cyclodextrin Glucanotransferase: A Comprehensive Guide from Gene to Applications explores the essential role of cyclodextrin glucanotransferase (CGTase) in starch degradation and its diverse industrial applications. This book serves as a valuable resource for researchers and industry professionals, offering comprehensive insights from the genetic basis of CGTase to its practical applications. The book covers the production of CGTase by different bacterial genera, including Bacillus spp., and their purification strategies. It delves into the structural and functional characteristics of CGTase, highlighting its catalytic mechanisms, substrate specificity, and factors affecting enzyme activity and stability. Additionally, the book explores recombinant CGTases, their properties, and advancements in protein engineering and enzyme immobilization. With a strong focus on practical applications, the book discusses the remarkable properties of cyclodextrins, such as inclusion complex formation, and their wide range of uses in various industries, including pharmaceuticals, textiles, bioremediation, and food. Detailed case studies and real-world examples illustrate how CGTase and cyclodextrins can address specific challenges and drive innovation in these fields.
This book is an essential resource for applied science researchers working in the field of starch processing enzymes, providing them with the knowledge to advance their research and development. It also serves as a valuable reference for researchers in the starch processing industries, offering insights into the latest advancements and practical applications of CGTase and cyclodextrins.
1. Section I: Introduction - p. S1 2. Chapter 1. Introduction to α-amylase family - p. 3 3. Chapter 2. Cyclodextrin gluconotransferase: an overview - p. 25 4. Chapter 3. Structure of cyclodextrin glucanotransferases - p. 57 5. Section II: Mechanism and cyclodextrin glucanotransferase production - p. S83 6. Chapter 4. Catalytic mechanisms of cyclodextrin glucanotransferases - p. 85 7. Cyclodextrin glucanotransferase from Bacillus spp. - p. 109 8. Cyclodextrin glucanotransferase from other than Bacillus genera - p. 133 9. Section III: Purification and engineering of cyclodextrin glucanotransferases - p. S167 10. Chapter 7. Cloning and overexpression of cyclodextrin glucanotransferase - p. 169 11. Chapter 8. Extraction, purification, and characterization of cyclodextrin glucanotransferases - p. 195 12. Chapter 9. Protein engineering of cyclodextrin glucanotransferases - p. 225 13. Chapter 10. Immobilization of cyclodextrin glucanotransferases - p. 251 14. Section IV: Cyclodextrins and their applications - p. S283 15. Chapter 11. Structure and properties of cyclodextrins - p. 285 16. Chapter 12. Current status of cyclodextrin production - p. 307 17. Chapter 13. Large-ring cyclodextrins - p. 329 18. Chapter 14. Applications of cyclodextrins - p. 353 19. Index - p. 381
Cyclodextrin Glucanotransferase: A Comprehensive Guide from Gene to Applications explores the essential role of cyclodextrin glucanotransferase (CGTase) in starch degradation and its diverse industrial applications. This book serves as a valuable resource for researchers and industry professionals, offering comprehensive insights from the genetic basis of CGTase to its practical applications. The book covers the production of CGTase by different bacterial genera, including Bacillus spp., and their purification strategies. It delves into the structural and functional characteristics of CGTase, highlighting its catalytic mechanisms, substrate specificity, and factors affecting enzyme activity and stability. Additionally, the book explores recombinant CGTases, their properties, and advancements in protein engineering and enzyme immobilization. With a strong focus on practical applications, the book discusses the remarkable properties of cyclodextrins, such as inclusion complex formation, and their wide range of uses in various industries, including pharmaceuticals, textiles, bioremediation, and food. Detailed case studies and real-world examples illustrate how CGTase and cyclodextrins can address specific challenges and drive innovation in these fields.
This book is an essential resource for applied science researchers working in the field of starch processing enzymes, providing them with the knowledge to advance their research and development. It also serves as a valuable reference for researchers in the starch processing industries, offering insights into the latest advancements and practical applications of CGTase and cyclodextrins.
1. Section I: Introduction - p. S1 2. Chapter 1. Introduction to α-amylase family - p. 3 3. Chapter 2. Cyclodextrin gluconotransferase: an overview - p. 25 4. Chapter 3. Structure of cyclodextrin glucanotransferases - p. 57 5. Section II: Mechanism and cyclodextrin glucanotransferase production - p. S83 6. Chapter 4. Catalytic mechanisms of cyclodextrin glucanotransferases - p. 85 7. Cyclodextrin glucanotransferase from Bacillus spp. - p. 109 8. Cyclodextrin glucanotransferase from other than Bacillus genera - p. 133 9. Section III: Purification and engineering of cyclodextrin glucanotransferases - p. S167 10. Chapter 7. Cloning and overexpression of cyclodextrin glucanotransferase - p. 169 11. Chapter 8. Extraction, purification, and characterization of cyclodextrin glucanotransferases - p. 195 12. Chapter 9. Protein engineering of cyclodextrin glucanotransferases - p. 225 13. Chapter 10. Immobilization of cyclodextrin glucanotransferases - p. 251 14. Section IV: Cyclodextrins and their applications - p. S283 15. Chapter 11. Structure and properties of cyclodextrins - p. 285 16. Chapter 12. Current status of cyclodextrin production - p. 307 17. Chapter 13. Large-ring cyclodextrins - p. 329 18. Chapter 14. Applications of cyclodextrins - p. 353 19. Index - p. 381
