Diagnostic Molecular Biology, Second Edition describes the fundamentals of molecular biology in a clear, concise manner with each technique explained within its conceptual framework and current applications of clinical laboratory techniques comprehensively covered. This targeted approach covers the principles of molecular biology, including basic knowledge of nucleic acids, proteins and chromosomes; the basic techniques and instrumentations commonly used in the field of molecular biology, including detailed procedures and explanations; and the applications of the principles and techniques currently employed in the clinical laboratory. Topics such as whole exome sequencing, whole genome sequencing, RNA-seq, and ChIP-seq round out the discussion.
Fully updated, this new edition adds recent advances in the detection of respiratory virus infections in humans, like influenza, RSV, hAdV, hRV but also corona. This book expands the discussion on NGS application and its role in future precision medicine.
1. Diagnostic Molecular Biology - p. Page i 2. Cover - p. Page i 3. Front Matter - p. Page ii 4. Table of Contents - p. Page iii 5. Copyright - p. Page iv 6. Dedication - p. Page v 7. Preface - p. Page vi 8. Acknowledgement - p. Page vii 9. List of Illustrations - p. Page viii 10. List of Tables - p. Page ix 11. Chapter 1 : Nucleic acids: DNA and RNA - p. Page ix 12. Chapter 2 : Nucleic acid-based cellular activities – DNA replication, damage, and repair - p. Page 26 13. Chapter 3 : Gene expression: transcription of the genetic code - p. Page 56 14. Chapter 4 : Gene expression: translation of the genetic code - p. Page 89 15. Chapter 5 : The genome - p. Page 125 16. Chapter 6 : Extraction and purification of nucleic acids - p. Page 155 17. Chapter 7 : Quantification and analysis of nucleic acids - p. Page 180 18. Chapter 8 : Extraction and purification of proteins - p. Page 208 19. Chapter 9 : Quantification and analysis of proteins - p. Page 229 20. Chapter 10 : Amplification of nucleic acids - p. Page 257 21. Chapter 11 : Characterization of nucleic acids and proteins - p. Page 292 22. Chapter 12 : Techniques in sequencing - p. Page 330 23. Chapter 13 : Genome and transcriptome analysis - p. Page 365 24. Chapter 14 : Molecular diagnosis of chromosomal disorders - p. Page 391 25. Chapter 15 : Molecular diagnosis of gene mutation and inherited diseases - p. Page 423 26. Chapter 16 : Molecular diagnosis of infectious diseases - p. Page 455 27. Chapter 17 : The biology of SARS-CoV-2 and the molecular diagnosis of COVID-19 - p. Page 490 28. Chapter 18 : Guidance for molecular diagnostic laboratory - p. Page 519 29. Bibliography - p. Page 541 30. Index - p. Page 541
Diagnostic Molecular Biology, Second Edition describes the fundamentals of molecular biology in a clear, concise manner with each technique explained within its conceptual framework and current applications of clinical laboratory techniques comprehensively covered. This targeted approach covers the principles of molecular biology, including basic knowledge of nucleic acids, proteins and chromosomes; the basic techniques and instrumentations commonly used in the field of molecular biology, including detailed procedures and explanations; and the applications of the principles and techniques currently employed in the clinical laboratory. Topics such as whole exome sequencing, whole genome sequencing, RNA-seq, and ChIP-seq round out the discussion.
Fully updated, this new edition adds recent advances in the detection of respiratory virus infections in humans, like influenza, RSV, hAdV, hRV but also corona. This book expands the discussion on NGS application and its role in future precision medicine.
1. Diagnostic Molecular Biology - p. Page i 2. Cover - p. Page i 3. Front Matter - p. Page ii 4. Table of Contents - p. Page iii 5. Copyright - p. Page iv 6. Dedication - p. Page v 7. Preface - p. Page vi 8. Acknowledgement - p. Page vii 9. List of Illustrations - p. Page viii 10. List of Tables - p. Page ix 11. Chapter 1 : Nucleic acids: DNA and RNA - p. Page ix 12. Chapter 2 : Nucleic acid-based cellular activities – DNA replication, damage, and repair - p. Page 26 13. Chapter 3 : Gene expression: transcription of the genetic code - p. Page 56 14. Chapter 4 : Gene expression: translation of the genetic code - p. Page 89 15. Chapter 5 : The genome - p. Page 125 16. Chapter 6 : Extraction and purification of nucleic acids - p. Page 155 17. Chapter 7 : Quantification and analysis of nucleic acids - p. Page 180 18. Chapter 8 : Extraction and purification of proteins - p. Page 208 19. Chapter 9 : Quantification and analysis of proteins - p. Page 229 20. Chapter 10 : Amplification of nucleic acids - p. Page 257 21. Chapter 11 : Characterization of nucleic acids and proteins - p. Page 292 22. Chapter 12 : Techniques in sequencing - p. Page 330 23. Chapter 13 : Genome and transcriptome analysis - p. Page 365 24. Chapter 14 : Molecular diagnosis of chromosomal disorders - p. Page 391 25. Chapter 15 : Molecular diagnosis of gene mutation and inherited diseases - p. Page 423 26. Chapter 16 : Molecular diagnosis of infectious diseases - p. Page 455 27. Chapter 17 : The biology of SARS-CoV-2 and the molecular diagnosis of COVID-19 - p. Page 490 28. Chapter 18 : Guidance for molecular diagnostic laboratory - p. Page 519 29. Bibliography - p. Page 541 30. Index - p. Page 541
