DNA Damage and Chromosomal Instability, Volume 182 in the Methods in Cell Biology series, presents interesting chapters on a variety of timely topics, including Induction of chromosome-specific micronuclei and chromothripsis by centromere inactivation, Generation of aneuploid cells and assessment of their ability to survive in presence of chemotherapeutic agents, Genetic reporters to detect and quantify homologous recombination in yeast, Mapping histone variant genomic distribution: exploiting SNAP- Tag labelling to follow the dynamics of incorporation of H3 variants, Enrichment of DNA replication intermediates by EdU pull down, Nascent DNA sequencing and its diverse applications in genome integrity research, and much more. Additional chapters cover Enrichment of DNA replication intermediates by EdU pull down, DNA curtains for studying phase separation mechanisms of DNA-organizing proteins, Visualizing the Dynamics of DNA Replication and Repair at the Single-Molecule Molecule Level, Cloning and Expansion of Repetitive DNA Sequences, Using an ImageJ-based script to detect replication stress and associated cell cycle exit from G2 phase by fluorescence microscopy Arne Lindqvist
1. Cover image - p. i 2. Table of Contents - p. iii 3. Series Page - p. iii 4. Copyright - p. iii 5. Contributors - p. iii 6. DNA damage and chromosomal instability - p. xvii 7. Chapter 1: Induction of chromosome-specific micronuclei and chromothripsis by centromere inactivation - p. 1 8. Chapter 2: Generation of aneuploid cells and assessment of their ability to survive in presence of chemotherapeutic agents - p. 21 9. Chapter 3: Genetic reporters to detect and quantify homologous recombination in yeast - p. 35 10. Chapter 4: Mapping histone variant genomic distribution: Exploiting SNAP-tag labeling to follow the dynamics of incorporation of H3 variants - p. 49 11. Chapter 5: Nascent DNA sequencing and its diverse applications in genome integrity research - p. 67 12. Chapter 6: Enrichment of DNA replication intermediates by EdU pull down - p. 83 13. Chapter 7: DNA curtains for studying phase separation mechanisms of DNA-organizing proteins - p. 95 14. Chapter 8: Visualizing the dynamics of DNA replication and repair at the single-molecule level - p. 109 15. Chapter 9: Cloning and expansion of repetitive DNA sequences - p. 167 16. Chapter 10: Using an ImageJ-based script to detect replication stress and associated cell cycle exit from G2 phase by fluorescence microscopy - p. 187 17. Chapter 11: An automated image analysis pipeline to quantify the coordination and overlap of transcription and replication activity in mammalian genomes - p. 199 18. Chapter 12: Monitoring Chk1 kinase activity dynamics in live single cell imaging assays - p. 221 19. Chapter 13: Visualizing DNA damage and repair using single molecule super resolution microscopy - p. 237 20. Chapter 14: Quantitative analysis of nuclear deformations and DNA damage foci dynamics by live-cell imaging - p. 247 21. Chapter 15: Native FISH: A low- and high-throughput assay to analyze the alternative lengthening of telomere (ALT) pathway - p. 265 22. Chapter 16: Assessment of DNA fibers to track replication dynamics - p. 285 23. Chapter 17: A high-content flow cytometry and dual CRISPR-Cas9 based platform to quantify genetic interactions - p. 299 24. Chapter 18: Monitoring APOBEC3A protein levels in human cancer cells - p. 313
DNA Damage and Chromosomal Instability, Volume 182 in the Methods in Cell Biology series, presents interesting chapters on a variety of timely topics, including Induction of chromosome-specific micronuclei and chromothripsis by centromere inactivation, Generation of aneuploid cells and assessment of their ability to survive in presence of chemotherapeutic agents, Genetic reporters to detect and quantify homologous recombination in yeast, Mapping histone variant genomic distribution: exploiting SNAP- Tag labelling to follow the dynamics of incorporation of H3 variants, Enrichment of DNA replication intermediates by EdU pull down, Nascent DNA sequencing and its diverse applications in genome integrity research, and much more. Additional chapters cover Enrichment of DNA replication intermediates by EdU pull down, DNA curtains for studying phase separation mechanisms of DNA-organizing proteins, Visualizing the Dynamics of DNA Replication and Repair at the Single-Molecule Molecule Level, Cloning and Expansion of Repetitive DNA Sequences, Using an ImageJ-based script to detect replication stress and associated cell cycle exit from G2 phase by fluorescence microscopy Arne Lindqvist
1. Cover image - p. i 2. Table of Contents - p. iii 3. Series Page - p. iii 4. Copyright - p. iii 5. Contributors - p. iii 6. DNA damage and chromosomal instability - p. xvii 7. Chapter 1: Induction of chromosome-specific micronuclei and chromothripsis by centromere inactivation - p. 1 8. Chapter 2: Generation of aneuploid cells and assessment of their ability to survive in presence of chemotherapeutic agents - p. 21 9. Chapter 3: Genetic reporters to detect and quantify homologous recombination in yeast - p. 35 10. Chapter 4: Mapping histone variant genomic distribution: Exploiting SNAP-tag labeling to follow the dynamics of incorporation of H3 variants - p. 49 11. Chapter 5: Nascent DNA sequencing and its diverse applications in genome integrity research - p. 67 12. Chapter 6: Enrichment of DNA replication intermediates by EdU pull down - p. 83 13. Chapter 7: DNA curtains for studying phase separation mechanisms of DNA-organizing proteins - p. 95 14. Chapter 8: Visualizing the dynamics of DNA replication and repair at the single-molecule level - p. 109 15. Chapter 9: Cloning and expansion of repetitive DNA sequences - p. 167 16. Chapter 10: Using an ImageJ-based script to detect replication stress and associated cell cycle exit from G2 phase by fluorescence microscopy - p. 187 17. Chapter 11: An automated image analysis pipeline to quantify the coordination and overlap of transcription and replication activity in mammalian genomes - p. 199 18. Chapter 12: Monitoring Chk1 kinase activity dynamics in live single cell imaging assays - p. 221 19. Chapter 13: Visualizing DNA damage and repair using single molecule super resolution microscopy - p. 237 20. Chapter 14: Quantitative analysis of nuclear deformations and DNA damage foci dynamics by live-cell imaging - p. 247 21. Chapter 15: Native FISH: A low- and high-throughput assay to analyze the alternative lengthening of telomere (ALT) pathway - p. 265 22. Chapter 16: Assessment of DNA fibers to track replication dynamics - p. 285 23. Chapter 17: A high-content flow cytometry and dual CRISPR-Cas9 based platform to quantify genetic interactions - p. 299 24. Chapter 18: Monitoring APOBEC3A protein levels in human cancer cells - p. 313
