Dyneins: The Biology of Dynein Motors, Second Edition, offers a broad view of dyneins from structure, composition and organization, to biology of dynein function in both cytoplasm and cilia. As the second book in a pair on this topic, these works provide an overview of dyneins, from structure and function, to dysfunction and disease. Since the first edition, enormous strides have been taken in understanding dynein structure, its organization in the axoneme, single molecule motor mechanics and the consequences of defects for human biology, disease and development. This second edition is extensively revised, with coverage expanded from 24 to 42 chapters.
Much of the expanded coverage occurs in Volume Two on dynein dysfunction and disease, such as the role of dynein and cancer, while Volume One covers the history and evolution of dyneins, dyneins in ciliary biology and cytoplasmic dynein biology.
1. 1.1. Introduction - p. 5 2. 1.2. Research at Harvard - p. 9 3. 1.3. Research at the University of Hawaii - p. 20 4. 1.4. Semiretirement in Berkeley - p. 62 5. 1.5. The pluses of working on a minus-end directed motor - p. 76 6. 2. Origins of cytoplasmic dynein - p. 88 7. 3. The evolutionary biology of dyneins - p. 100 8. Part II. Dyneins in Ciliary Biology - p. 138 9. 4. Cytoplasmic preassembly and trafficking of axonemal dyneins - p. 140 10. 5. Composition and assembly of axonemal dyneins - p. 162 11. 5.1. Introduction - p. 163 12. 5.2. Classes of dynein components - p. 164 13. 5.3. Monomeric inner dynein arms - p. 166 14. 5.4. Dimeric inner dynein arm I1/f - p. 166 15. 5.5. Outer dynein arms - p. 172 16. 5.6. Interdynein linkers - p. 174 17. 5.7. Properties and organization of axonemal dynein motor units - p. 176 18. 5.8. Core WD-repeat intermediate chains associated with oligomeric motors - p. 178 19. 5.9. Additional intermediate chains - p. 179 20. 5.10. Core light chains associated with oligomeric motors - p. 180 21. 5.11. Regulatory components - p. 184 22. 5.12. Docking motors onto the axoneme - p. 188 23. 5.13. Other dynein-associated components - p. 191 24. 5.14. Conclusions - p. 192 25. 6. Organization of dyneins in the axoneme - p. 202 26. 7. Genetic approaches to axonemal dynein function in Chlamydomonas and other organisms - p. 218 27. 7.1. Introduction - p. 219 28. 7.2. Genetic studies of Chlamydomonas axonemal dyneins - p. 220 29. 7.3. Genetic studies in various organisms - p. 227 30. 7.4. Conclusion and perspective - p. 238 31. 8. Regulatory mechanics of outer-arm dynein motors - p. 250 32. 9. Control of axonemal inner dynein arms - p. 270 33. 9.1. Overview - p. 271 34. 9.2. Organization and assembly of the inner dynein arms - p. 274 35. 9.3. Functional role of I1 dynein and dynein c - p. 280 36. 9.4. Regulation of I1 dynein - p. 281 37. 9.5. New questions - p. 285 38. 10. Ciliary and flagellar motility and the nexin-dynein regulatory complex - p. 298 39. 10.1. Introduction - p. 299 40. 10.2. The central pair, radial spokes, and dynein regulatory complex - p. 301 41. 10.3. The dynein heavy chain suppressors - p. 304 42. 10.4. The dynein regulatory complex and the inner dynein arms - p. 309 43. 10.5. The dynein regulatory complex and nexin link - p. 312 44. 10.6. Identification and localization of dynein regulatory complex subunits within the nexin link - p. 314 45. 10.7. Identification of polypeptides that may interact with the nexin–dynein regulatory complex - p. 320 46. 10.8. Function of the DRC–nexin link in motility and future directions - p. 321 47. 11. Regulation of dynein-driven ciliary and flagellar movement - p. 336 48. 12. Dynein-mediated photobehavioral responses in Chlamydomonas - p. 368 49. 13. Dynein and intraflagellar transport - p. 386 50. 13.1. Introduction - p. 387 51. 13.2. Intraflagellar transport - p. 389 52. 13.3. Discovery of the cytoplasmic dynein 2 heavy chain and early proposals for its function - p. 391 53. 13.4. Identification of cytoplasmic dynein 2 as the retrograde intraflagellar transport motor - p. 392 54. 13.5. Structure and subunit content of cytoplasmic dynein 2 - p. 396 55. 13.6. Variations on the theme: multiple dynein 2 HCs and a possible alternative dynein for retrograde IFT - p. 412 56. 13.7. Biological functions of cytoplasmic dynein 2 and retrograde intraflagellar transport - p. 413 57. 13.8. Cytoplasmic function of dynein 2 - p. 421 58. 13.9. Regulation of dynein 2 expression, localization, and function - p. 423 59. 13.10. Conclusion - p. 424 60. Part III. Cytoplasmic Dynein Biology - p. 432 61. 14. Cytoplasmic dynein function defined by subunit composition - p. 434 62. 15. Regulation of cytoplasmic dynein motility - p. 450 63. 16. Insights into cytoplasmic dynein function and regulation from fungal genetics - p. 470 64. 16.1. Introduction - p. 471 65. 16.2. Discoveries of dynein function in spindle orientation/nuclear migration - p. 472 66. 16.3. Identification of dynein regulators using fungal genetics - p. 476 67. 16.4. Dissecting the mechanism and function of the microtubule plus-end accumulation of cytoplasmic dynein - p. 478 68. 16.5. Understanding the functions of various components of the dynein and dynactin complexes - p. 482 69. 16.6. Identification of proteins required for dynein–cargo interaction - p. 485 70. 16.7. Conclusions - p. 488 71. 17. Role of dynactin in dynein-mediated motility - p. 502 72. 18. Role of cytoplasmic dynein and dynactin in mitotic checkpoint silencing - p. 516 73. 19. Cytoplasmic dynein during mitosis - p. 534 74. 20. Dynein and dynactin at microtubule plus ends - p. 556 75. 21. Drosophila cytoplasmic dynein: Mutations, tools, and developmental functions - p. 568 76. 21.1. Introduction - p. 569 77. 21.2. Drosophila dynein genes and mutations - p. 574 78. 21.3. Drosophila as a model system: tools and advantages - p. 576 79. 21.4. Dynein function in gametogenesis - p. 580 80. 21.5. Dynein function in embryogenesis - p. 590 81. 21.6. Dynein function in neurons - p. 600 82. 21.7. Conclusion - p. 610 83. Index - p. 629
Dyneins: The Biology of Dynein Motors, Second Edition, offers a broad view of dyneins from structure, composition and organization, to biology of dynein function in both cytoplasm and cilia. As the second book in a pair on this topic, these works provide an overview of dyneins, from structure and function, to dysfunction and disease. Since the first edition, enormous strides have been taken in understanding dynein structure, its organization in the axoneme, single molecule motor mechanics and the consequences of defects for human biology, disease and development. This second edition is extensively revised, with coverage expanded from 24 to 42 chapters.
Much of the expanded coverage occurs in Volume Two on dynein dysfunction and disease, such as the role of dynein and cancer, while Volume One covers the history and evolution of dyneins, dyneins in ciliary biology and cytoplasmic dynein biology.
1. 1.1. Introduction - p. 5 2. 1.2. Research at Harvard - p. 9 3. 1.3. Research at the University of Hawaii - p. 20 4. 1.4. Semiretirement in Berkeley - p. 62 5. 1.5. The pluses of working on a minus-end directed motor - p. 76 6. 2. Origins of cytoplasmic dynein - p. 88 7. 3. The evolutionary biology of dyneins - p. 100 8. Part II. Dyneins in Ciliary Biology - p. 138 9. 4. Cytoplasmic preassembly and trafficking of axonemal dyneins - p. 140 10. 5. Composition and assembly of axonemal dyneins - p. 162 11. 5.1. Introduction - p. 163 12. 5.2. Classes of dynein components - p. 164 13. 5.3. Monomeric inner dynein arms - p. 166 14. 5.4. Dimeric inner dynein arm I1/f - p. 166 15. 5.5. Outer dynein arms - p. 172 16. 5.6. Interdynein linkers - p. 174 17. 5.7. Properties and organization of axonemal dynein motor units - p. 176 18. 5.8. Core WD-repeat intermediate chains associated with oligomeric motors - p. 178 19. 5.9. Additional intermediate chains - p. 179 20. 5.10. Core light chains associated with oligomeric motors - p. 180 21. 5.11. Regulatory components - p. 184 22. 5.12. Docking motors onto the axoneme - p. 188 23. 5.13. Other dynein-associated components - p. 191 24. 5.14. Conclusions - p. 192 25. 6. Organization of dyneins in the axoneme - p. 202 26. 7. Genetic approaches to axonemal dynein function in Chlamydomonas and other organisms - p. 218 27. 7.1. Introduction - p. 219 28. 7.2. Genetic studies of Chlamydomonas axonemal dyneins - p. 220 29. 7.3. Genetic studies in various organisms - p. 227 30. 7.4. Conclusion and perspective - p. 238 31. 8. Regulatory mechanics of outer-arm dynein motors - p. 250 32. 9. Control of axonemal inner dynein arms - p. 270 33. 9.1. Overview - p. 271 34. 9.2. Organization and assembly of the inner dynein arms - p. 274 35. 9.3. Functional role of I1 dynein and dynein c - p. 280 36. 9.4. Regulation of I1 dynein - p. 281 37. 9.5. New questions - p. 285 38. 10. Ciliary and flagellar motility and the nexin-dynein regulatory complex - p. 298 39. 10.1. Introduction - p. 299 40. 10.2. The central pair, radial spokes, and dynein regulatory complex - p. 301 41. 10.3. The dynein heavy chain suppressors - p. 304 42. 10.4. The dynein regulatory complex and the inner dynein arms - p. 309 43. 10.5. The dynein regulatory complex and nexin link - p. 312 44. 10.6. Identification and localization of dynein regulatory complex subunits within the nexin link - p. 314 45. 10.7. Identification of polypeptides that may interact with the nexin–dynein regulatory complex - p. 320 46. 10.8. Function of the DRC–nexin link in motility and future directions - p. 321 47. 11. Regulation of dynein-driven ciliary and flagellar movement - p. 336 48. 12. Dynein-mediated photobehavioral responses in Chlamydomonas - p. 368 49. 13. Dynein and intraflagellar transport - p. 386 50. 13.1. Introduction - p. 387 51. 13.2. Intraflagellar transport - p. 389 52. 13.3. Discovery of the cytoplasmic dynein 2 heavy chain and early proposals for its function - p. 391 53. 13.4. Identification of cytoplasmic dynein 2 as the retrograde intraflagellar transport motor - p. 392 54. 13.5. Structure and subunit content of cytoplasmic dynein 2 - p. 396 55. 13.6. Variations on the theme: multiple dynein 2 HCs and a possible alternative dynein for retrograde IFT - p. 412 56. 13.7. Biological functions of cytoplasmic dynein 2 and retrograde intraflagellar transport - p. 413 57. 13.8. Cytoplasmic function of dynein 2 - p. 421 58. 13.9. Regulation of dynein 2 expression, localization, and function - p. 423 59. 13.10. Conclusion - p. 424 60. Part III. Cytoplasmic Dynein Biology - p. 432 61. 14. Cytoplasmic dynein function defined by subunit composition - p. 434 62. 15. Regulation of cytoplasmic dynein motility - p. 450 63. 16. Insights into cytoplasmic dynein function and regulation from fungal genetics - p. 470 64. 16.1. Introduction - p. 471 65. 16.2. Discoveries of dynein function in spindle orientation/nuclear migration - p. 472 66. 16.3. Identification of dynein regulators using fungal genetics - p. 476 67. 16.4. Dissecting the mechanism and function of the microtubule plus-end accumulation of cytoplasmic dynein - p. 478 68. 16.5. Understanding the functions of various components of the dynein and dynactin complexes - p. 482 69. 16.6. Identification of proteins required for dynein–cargo interaction - p. 485 70. 16.7. Conclusions - p. 488 71. 17. Role of dynactin in dynein-mediated motility - p. 502 72. 18. Role of cytoplasmic dynein and dynactin in mitotic checkpoint silencing - p. 516 73. 19. Cytoplasmic dynein during mitosis - p. 534 74. 20. Dynein and dynactin at microtubule plus ends - p. 556 75. 21. Drosophila cytoplasmic dynein: Mutations, tools, and developmental functions - p. 568 76. 21.1. Introduction - p. 569 77. 21.2. Drosophila dynein genes and mutations - p. 574 78. 21.3. Drosophila as a model system: tools and advantages - p. 576 79. 21.4. Dynein function in gametogenesis - p. 580 80. 21.5. Dynein function in embryogenesis - p. 590 81. 21.6. Dynein function in neurons - p. 600 82. 21.7. Conclusion - p. 610 83. Index - p. 629
