Volume 608 of the series Methods in Enzymology covers key aspects of enzyme discovery, engineering tools and platforms, and examples of applications in the enzymology of synthetic biology.
1. Cover image - p. iv 2. Title page - p. iv 3. Table of Contents - p. iv 4. Copyright - p. iv 5. Contributors - p. xi 6. Preface - p. xvii 7. Section I: Enzyme Discovery - p. xviii 8. Chapter One: Enzyme Discovery: Enzyme Selection and Pathway Design - p. xviii 9. Section II: Enzyme Engineering Tools and Platforms - p. 27 10. Chapter Two: Cell-Free Synthetic Biology for Pathway Prototyping - p. 27 11. Chapter Three: Fast and Flexible Synthesis of Combinatorial Libraries for Directed Evolution - p. 57 12. Section III: Enzyme Families: Parts and Platforms for Chemical Diversity - p. 79 13. Chapter Four: Sesquiterpene Synthase-Catalyzed Conversion of a Farnesyl Diphosphate Analogue to a Nonnatural Terpenoid Ether - p. 79 14. Chapter Five: In Vivo Platforms for Terpenoid Overproduction and the Generation of Chemical Diversity - p. 95 15. Chapter Six: Imine Reductases, Reductive Aminases, and Amine Oxidases for the Synthesis of Chiral Amines: Discovery, Characterization, and Synthetic Applications - p. 129 16. Chapter Seven: Experimental Protocols for Generating Focused Mutant Libraries and Screening for Thermostable Proteins - p. 149 17. Chapter Eight: Characterization of Cytochrome P450 Enzymes and Their Applications in Synthetic Biology - p. 187 18. Section IV: Genome Engineering for Enzyme Pathways - p. 261 19. Chapter Nine: Metabolic Engineering of Saccharomyces cerevisiae Using a Trifunctional CRISPR/Cas System for Simultaneous Gene Activation, Interference, and Deletion - p. 261 20. Chapter Ten: YeastFab: High-Throughput Genetic Parts Construction, Measurement, and Pathway Engineering in Yeast - p. 276 21. Section V: Bioengineered Strains with Designed Enzyme Pathways - p. 306 22. Chapter Eleven: Halomonas and Pathway Engineering for Bioplastics Production - p. 306 23. Chapter Twelve: An Engineering Approach for Rewiring Microbial Metabolism - p. 328 24. Chapter Thirteen: Multifragment DNA Assembly of Biochemical Pathways via Automated Ligase Cycling Reaction - p. 367 25. Chapter Fourteen: Commodity Chemicals From Engineered Modular Type I Polyketide Synthases - p. 392 26. Chapter Fifteen: Modular Pathway Rewiring of Yeast for Amino Acid Production - p. 415
Volume 608 of the series Methods in Enzymology covers key aspects of enzyme discovery, engineering tools and platforms, and examples of applications in the enzymology of synthetic biology.
1. Cover image - p. iv 2. Title page - p. iv 3. Table of Contents - p. iv 4. Copyright - p. iv 5. Contributors - p. xi 6. Preface - p. xvii 7. Section I: Enzyme Discovery - p. xviii 8. Chapter One: Enzyme Discovery: Enzyme Selection and Pathway Design - p. xviii 9. Section II: Enzyme Engineering Tools and Platforms - p. 27 10. Chapter Two: Cell-Free Synthetic Biology for Pathway Prototyping - p. 27 11. Chapter Three: Fast and Flexible Synthesis of Combinatorial Libraries for Directed Evolution - p. 57 12. Section III: Enzyme Families: Parts and Platforms for Chemical Diversity - p. 79 13. Chapter Four: Sesquiterpene Synthase-Catalyzed Conversion of a Farnesyl Diphosphate Analogue to a Nonnatural Terpenoid Ether - p. 79 14. Chapter Five: In Vivo Platforms for Terpenoid Overproduction and the Generation of Chemical Diversity - p. 95 15. Chapter Six: Imine Reductases, Reductive Aminases, and Amine Oxidases for the Synthesis of Chiral Amines: Discovery, Characterization, and Synthetic Applications - p. 129 16. Chapter Seven: Experimental Protocols for Generating Focused Mutant Libraries and Screening for Thermostable Proteins - p. 149 17. Chapter Eight: Characterization of Cytochrome P450 Enzymes and Their Applications in Synthetic Biology - p. 187 18. Section IV: Genome Engineering for Enzyme Pathways - p. 261 19. Chapter Nine: Metabolic Engineering of Saccharomyces cerevisiae Using a Trifunctional CRISPR/Cas System for Simultaneous Gene Activation, Interference, and Deletion - p. 261 20. Chapter Ten: YeastFab: High-Throughput Genetic Parts Construction, Measurement, and Pathway Engineering in Yeast - p. 276 21. Section V: Bioengineered Strains with Designed Enzyme Pathways - p. 306 22. Chapter Eleven: Halomonas and Pathway Engineering for Bioplastics Production - p. 306 23. Chapter Twelve: An Engineering Approach for Rewiring Microbial Metabolism - p. 328 24. Chapter Thirteen: Multifragment DNA Assembly of Biochemical Pathways via Automated Ligase Cycling Reaction - p. 367 25. Chapter Fourteen: Commodity Chemicals From Engineered Modular Type I Polyketide Synthases - p. 392 26. Chapter Fifteen: Modular Pathway Rewiring of Yeast for Amino Acid Production - p. 415
