Enzymes of Epigenetics, one of two new volumes in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field.
This volume covers research methods that are employed to study epigenetic regulation and includes structural, biochemical, molecular, biological, cellular, computational, and systems approaches.
Topics include chromatin structure and histones, posttranslational histone modification enzymes and complexes, histone modification binders, DNA modifications and nucleic acid regulators, epigenetic technologies and small molecule epigenetic regulators, and biological connections
1. Cover image - p. iv 2. Title page - p. iv 3. Table of Contents - p. iv 4. Copyright - p. iv 5. Contributors - p. xiii 6. Preface - p. xvii 7. Part I: Chromatin Structure and Histones - p. xix 8. Chapter One: In Vitro Chromatin Assembly: Strategies and Quality Control - p. xix 9. Chapter Two: Preparation, Crystallization, and Structure Determination of Chromatin Enzyme/Nucleosome Complexes - p. 41 10. Chapter Three: Preparation of Recombinant Centromeric Nucleosomes and Formation of Complexes with Nonhistone Centromere Proteins - p. 65 11. Chapter Four: Functional Characterization of Histone Chaperones Using SNAP-Tag-Based Imaging to Assess De Novo Histone Deposition - p. 96 12. Chapter Five: Analysis of Nucleosome Sliding by ATP-Dependent Chromatin Remodeling Enzymes - p. 117 13. Part II: Posttranslational Histone Modification Enzymes and Complexes - p. 135 14. Chapter Six: In Vitro Activity Assays for MYST Histone Acetyltransferases and Adaptation for High-Throughput Inhibitor Screening - p. 135 15. Chapter Seven: Preparation and Biochemical Analysis of Classical Histone Deacetylases - p. 160 16. Chapter Eight: Recombinant Preparation, Biochemical Analysis, and Structure Determination of Sirtuin Family Histone/Protein Deacylases - p. 181 17. Chapter Nine: Preparation, Biochemical Analysis, and Structure Determination of SET Domain Histone Methyltransferases - p. 208 18. Chapter Ten: Expression, Purification, and Biochemical Analysis of the LSD1/KDM1A Histone Demethylase - p. 240 19. Chapter Eleven: LSD1 Histone Demethylase Assays and Inhibition - p. 259 20. Chapter Twelve: Purification, Biochemical Analysis, and Structure Determination of JmjC Lysine Demethylases - p. 278 21. Chapter Thirteen: Preparation and Analysis of Native Chromatin-Modifying Complexes - p. 301 22. Part III: Histone Modification Binders (Readers) - p. 318 23. Chapter Fourteen: Preparation, Biochemical Analysis, and Structure Determination of the Bromodomain, an Acetyl-Lysine Binding Domain - p. 318 24. Chapter Fifteen: Preparation, Biochemical Analysis, and Structure Determination of Methyllysine Readers - p. 343 25. Part IV: DNA Modifications and Nucleic Acid Regulators - p. 362 26. Chapter Sixteen: Quantification of Oxidized 5-Methylcytosine Bases and TET Enzyme Activity - p. 362 27. Chapter Seventeen: Characterization of How DNA Modifications Affect DNA Binding by C2H2 Zinc Finger Proteins - p. 385 28. Chapter Eighteen: Crystallographic Studies of Telomerase - p. 401 29. Chapter Nineteen: Detection and Analysis of Long Noncoding RNAs - p. 419 30. Chapter Twenty: Identifying Centromeric RNAs Involved in Histone Dynamics In Vivo - p. 444 31. Author Index - p. 467 32. Subject Index - p. 497
Enzymes of Epigenetics, one of two new volumes in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field.
This volume covers research methods that are employed to study epigenetic regulation and includes structural, biochemical, molecular, biological, cellular, computational, and systems approaches.
Topics include chromatin structure and histones, posttranslational histone modification enzymes and complexes, histone modification binders, DNA modifications and nucleic acid regulators, epigenetic technologies and small molecule epigenetic regulators, and biological connections
1. Cover image - p. iv 2. Title page - p. iv 3. Table of Contents - p. iv 4. Copyright - p. iv 5. Contributors - p. xiii 6. Preface - p. xvii 7. Part I: Chromatin Structure and Histones - p. xix 8. Chapter One: In Vitro Chromatin Assembly: Strategies and Quality Control - p. xix 9. Chapter Two: Preparation, Crystallization, and Structure Determination of Chromatin Enzyme/Nucleosome Complexes - p. 41 10. Chapter Three: Preparation of Recombinant Centromeric Nucleosomes and Formation of Complexes with Nonhistone Centromere Proteins - p. 65 11. Chapter Four: Functional Characterization of Histone Chaperones Using SNAP-Tag-Based Imaging to Assess De Novo Histone Deposition - p. 96 12. Chapter Five: Analysis of Nucleosome Sliding by ATP-Dependent Chromatin Remodeling Enzymes - p. 117 13. Part II: Posttranslational Histone Modification Enzymes and Complexes - p. 135 14. Chapter Six: In Vitro Activity Assays for MYST Histone Acetyltransferases and Adaptation for High-Throughput Inhibitor Screening - p. 135 15. Chapter Seven: Preparation and Biochemical Analysis of Classical Histone Deacetylases - p. 160 16. Chapter Eight: Recombinant Preparation, Biochemical Analysis, and Structure Determination of Sirtuin Family Histone/Protein Deacylases - p. 181 17. Chapter Nine: Preparation, Biochemical Analysis, and Structure Determination of SET Domain Histone Methyltransferases - p. 208 18. Chapter Ten: Expression, Purification, and Biochemical Analysis of the LSD1/KDM1A Histone Demethylase - p. 240 19. Chapter Eleven: LSD1 Histone Demethylase Assays and Inhibition - p. 259 20. Chapter Twelve: Purification, Biochemical Analysis, and Structure Determination of JmjC Lysine Demethylases - p. 278 21. Chapter Thirteen: Preparation and Analysis of Native Chromatin-Modifying Complexes - p. 301 22. Part III: Histone Modification Binders (Readers) - p. 318 23. Chapter Fourteen: Preparation, Biochemical Analysis, and Structure Determination of the Bromodomain, an Acetyl-Lysine Binding Domain - p. 318 24. Chapter Fifteen: Preparation, Biochemical Analysis, and Structure Determination of Methyllysine Readers - p. 343 25. Part IV: DNA Modifications and Nucleic Acid Regulators - p. 362 26. Chapter Sixteen: Quantification of Oxidized 5-Methylcytosine Bases and TET Enzyme Activity - p. 362 27. Chapter Seventeen: Characterization of How DNA Modifications Affect DNA Binding by C2H2 Zinc Finger Proteins - p. 385 28. Chapter Eighteen: Crystallographic Studies of Telomerase - p. 401 29. Chapter Nineteen: Detection and Analysis of Long Noncoding RNAs - p. 419 30. Chapter Twenty: Identifying Centromeric RNAs Involved in Histone Dynamics In Vivo - p. 444 31. Author Index - p. 467 32. Subject Index - p. 497
