Fe-S Cluster Enzymes, Part A, Volume 595 is the first of two volumes focused on Fe-S cluster enzymes. New topics of note in this series include Electrochemistry of Fe/S Proteins, Genetic, biochemical and biophysical methods for studying Fe-S proteins and their assembly, Fluorescent reporters to track Fe-S cluster assembly and transfer reactions, Mechanism-based strategies for structural characterization of radical SAM reaction intermediates, Purification and Characterization of Recalcitrant Cobalamin-Dependent Radical S-adenosylmethionine Methylases, A polymerase with potential: the Fe-S cluster in Human DNA Primase, In Vitro Studies of Cellular Iron-sulfur Cluster Biosynthesis, Trafficking and Transport, and Fe-S cluster Hsp70 Chaperones: the ATPase cycle and protein interactions.
1. Cover image - p. iv 2. Title page - p. iv 3. Table of Contents - p. iv 4. Copyright - p. iv 5. Contributors - p. ix 6. Preface - p. xiii 7. Chapter One: Genetic, Biochemical, and Biophysical Methods for Studying FeS Proteins and Their Assembly - p. xv 8. Chapter Two: De Novo Design of Iron–Sulfur Proteins - p. 32 9. Chapter Three: In Vitro Studies of Cellular Iron–Sulfur Cluster Biosynthesis, Trafficking, and Transport - p. 53 10. Chapter Four: Combined Biochemical, Biophysical, and Cellular Methods to Study Fe–S Cluster Transfer and Cytosolic Aconitase Repair by MitoNEET - p. 82 11. Chapter Five: Defining the Architecture of the Core Machinery for the Assembly of Fe–S Clusters in Human Mitochondria - p. 106 12. Chapter Six: Fe–S Cluster Hsp70 Chaperones: The ATPase Cycle and Protein Interactions - p. 160 13. Chapter Seven: B. subtilis as a Model for Studying the Assembly of Fe–S Clusters in Gram-Positive Bacteria - p. 184 14. Chapter Eight: Structural Characterization of Poised States in the Oxygen Sensitive Hydrogenases and Nitrogenases - p. 212 15. Chapter Nine: Nitrogenase Assembly: Strategies and Procedures - p. 259 16. Chapter Ten: TsrM as a Model for Purifying and Characterizing Cobalamin-Dependent Radical S-Adenosylmethionine Methylases - p. 302 17. Chapter Eleven: Mechanism-Based Strategies for Structural Characterization of Radical SAM Reaction Intermediates - p. 329 18. Chapter Twelve: A Polymerase With Potential: The Fe–S Cluster in Human DNA Primase - p. 359 19. Author Index - p. 391 20. Subject Index - p. 417
Fe-S Cluster Enzymes, Part A, Volume 595 is the first of two volumes focused on Fe-S cluster enzymes. New topics of note in this series include Electrochemistry of Fe/S Proteins, Genetic, biochemical and biophysical methods for studying Fe-S proteins and their assembly, Fluorescent reporters to track Fe-S cluster assembly and transfer reactions, Mechanism-based strategies for structural characterization of radical SAM reaction intermediates, Purification and Characterization of Recalcitrant Cobalamin-Dependent Radical S-adenosylmethionine Methylases, A polymerase with potential: the Fe-S cluster in Human DNA Primase, In Vitro Studies of Cellular Iron-sulfur Cluster Biosynthesis, Trafficking and Transport, and Fe-S cluster Hsp70 Chaperones: the ATPase cycle and protein interactions.
1. Cover image - p. iv 2. Title page - p. iv 3. Table of Contents - p. iv 4. Copyright - p. iv 5. Contributors - p. ix 6. Preface - p. xiii 7. Chapter One: Genetic, Biochemical, and Biophysical Methods for Studying FeS Proteins and Their Assembly - p. xv 8. Chapter Two: De Novo Design of Iron–Sulfur Proteins - p. 32 9. Chapter Three: In Vitro Studies of Cellular Iron–Sulfur Cluster Biosynthesis, Trafficking, and Transport - p. 53 10. Chapter Four: Combined Biochemical, Biophysical, and Cellular Methods to Study Fe–S Cluster Transfer and Cytosolic Aconitase Repair by MitoNEET - p. 82 11. Chapter Five: Defining the Architecture of the Core Machinery for the Assembly of Fe–S Clusters in Human Mitochondria - p. 106 12. Chapter Six: Fe–S Cluster Hsp70 Chaperones: The ATPase Cycle and Protein Interactions - p. 160 13. Chapter Seven: B. subtilis as a Model for Studying the Assembly of Fe–S Clusters in Gram-Positive Bacteria - p. 184 14. Chapter Eight: Structural Characterization of Poised States in the Oxygen Sensitive Hydrogenases and Nitrogenases - p. 212 15. Chapter Nine: Nitrogenase Assembly: Strategies and Procedures - p. 259 16. Chapter Ten: TsrM as a Model for Purifying and Characterizing Cobalamin-Dependent Radical S-Adenosylmethionine Methylases - p. 302 17. Chapter Eleven: Mechanism-Based Strategies for Structural Characterization of Radical SAM Reaction Intermediates - p. 329 18. Chapter Twelve: A Polymerase With Potential: The Fe–S Cluster in Human DNA Primase - p. 359 19. Author Index - p. 391 20. Subject Index - p. 417
