Methods in Enzymology, Volume 599 is the second of two volumes focused on Fe-S cluster enzymes. Topics of interest in this new release include steps towards understanding mitochondrial Fe/S cluster biogenesis, iron sulfur clusters in zinc finger proteins, electrochemistry of Iron-sulfur enzymes, NRVS for Fe in biology and its experiment and basic interpretation,
methods for studying iron regulatory protein 1, an important protein in human iron metabolism, the characterization of glutaredoxin Fe-S cluster binding interactions using circular dichroism spectroscopy, fluorescent reporters to track Fe-S cluster assembly and transfer reactions, methods for studying the Fe-S cluster containing base excision repair glycosylase MUTYH, and more.
1. Cover image - p. iv 2. Title page - p. iv 3. Table of Contents - p. iv 4. Copyright - p. iv 5. Contributors - p. xi 6. Preface - p. xv 7. Chapter One: Iron–Sulfur Clusters in DNA Polymerases and Primases of Eukaryotes - p. xvii 8. Chapter Two: Fe–S Clusters and MutY Base Excision Repair Glycosylases: Purification, Kinetics, and DNA Affinity Measurements - p. 20 9. Chapter Three: Cellular Assays for Studying the Fe–S Cluster Containing Base Excision Repair Glycosylase MUTYH and Homologs - p. 68 10. Chapter Four: Iron–Sulfur Clusters in Zinc Finger Proteins - p. 99 11. Chapter Five: Methods for Studying Iron Regulatory Protein 1: An Important Protein in Human Iron Metabolism - p. 137 12. Chapter Six: Robust Production, Crystallization, Structure Determination, and Analysis of [Fe–S] Proteins: Uncovering Control of Electron Shuttling and Gating in the Respiratory Metabolism of Molybdopterin Guanine Dinucleotide Enzymes - p. 155 13. Chapter Seven: Biochemical Reconstitution and Spectroscopic Analysis of Iron–Sulfur Proteins - p. 196 14. Chapter Eight: Biochemical Analyses of Human Iron–Sulfur Protein Biogenesis and of Related Diseases - p. 226 15. Chapter Nine: Steps Toward Understanding Mitochondrial Fe/S Cluster Biogenesis - p. 263 16. Chapter Ten: Approaches to Interrogate the Role of Nucleotide Hydrolysis by Metal Trafficking NTPases: The Nbp35–Cfd1 Iron–Sulfur Cluster Scaffold as a Case Study - p. 292 17. Chapter Eleven: Characterization of Glutaredoxin Fe–S Cluster-Binding Interactions Using Circular Dichroism Spectroscopy - p. 325 18. Chapter Twelve: Conformationally Gated Electron Transfer in Nitrogenase. Isolation, Purification, and Characterization of Nitrogenase From Gluconacetobacter diazotrophicus - p. 353 19. Chapter Thirteen: Protein Film Electrochemistry of Iron–Sulfur Enzymes - p. 386 20. Chapter Fourteen: NRVS for Fe in Biology: Experiment and Basic Interpretation - p. 407 21. Chapter Fifteen: Advanced X-ray Spectroscopic Methods for Studying Iron–Sulfur-Containing Proteins and Model Complexes - p. 425 22. Author Index - p. 451 23. Subject Index - p. 481
Methods in Enzymology, Volume 599 is the second of two volumes focused on Fe-S cluster enzymes. Topics of interest in this new release include steps towards understanding mitochondrial Fe/S cluster biogenesis, iron sulfur clusters in zinc finger proteins, electrochemistry of Iron-sulfur enzymes, NRVS for Fe in biology and its experiment and basic interpretation,
methods for studying iron regulatory protein 1, an important protein in human iron metabolism, the characterization of glutaredoxin Fe-S cluster binding interactions using circular dichroism spectroscopy, fluorescent reporters to track Fe-S cluster assembly and transfer reactions, methods for studying the Fe-S cluster containing base excision repair glycosylase MUTYH, and more.
1. Cover image - p. iv 2. Title page - p. iv 3. Table of Contents - p. iv 4. Copyright - p. iv 5. Contributors - p. xi 6. Preface - p. xv 7. Chapter One: Iron–Sulfur Clusters in DNA Polymerases and Primases of Eukaryotes - p. xvii 8. Chapter Two: Fe–S Clusters and MutY Base Excision Repair Glycosylases: Purification, Kinetics, and DNA Affinity Measurements - p. 20 9. Chapter Three: Cellular Assays for Studying the Fe–S Cluster Containing Base Excision Repair Glycosylase MUTYH and Homologs - p. 68 10. Chapter Four: Iron–Sulfur Clusters in Zinc Finger Proteins - p. 99 11. Chapter Five: Methods for Studying Iron Regulatory Protein 1: An Important Protein in Human Iron Metabolism - p. 137 12. Chapter Six: Robust Production, Crystallization, Structure Determination, and Analysis of [Fe–S] Proteins: Uncovering Control of Electron Shuttling and Gating in the Respiratory Metabolism of Molybdopterin Guanine Dinucleotide Enzymes - p. 155 13. Chapter Seven: Biochemical Reconstitution and Spectroscopic Analysis of Iron–Sulfur Proteins - p. 196 14. Chapter Eight: Biochemical Analyses of Human Iron–Sulfur Protein Biogenesis and of Related Diseases - p. 226 15. Chapter Nine: Steps Toward Understanding Mitochondrial Fe/S Cluster Biogenesis - p. 263 16. Chapter Ten: Approaches to Interrogate the Role of Nucleotide Hydrolysis by Metal Trafficking NTPases: The Nbp35–Cfd1 Iron–Sulfur Cluster Scaffold as a Case Study - p. 292 17. Chapter Eleven: Characterization of Glutaredoxin Fe–S Cluster-Binding Interactions Using Circular Dichroism Spectroscopy - p. 325 18. Chapter Twelve: Conformationally Gated Electron Transfer in Nitrogenase. Isolation, Purification, and Characterization of Nitrogenase From Gluconacetobacter diazotrophicus - p. 353 19. Chapter Thirteen: Protein Film Electrochemistry of Iron–Sulfur Enzymes - p. 386 20. Chapter Fourteen: NRVS for Fe in Biology: Experiment and Basic Interpretation - p. 407 21. Chapter Fifteen: Advanced X-ray Spectroscopic Methods for Studying Iron–Sulfur-Containing Proteins and Model Complexes - p. 425 22. Author Index - p. 451 23. Subject Index - p. 481
