This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers fluorescence fluctuation spectroscopy and includes chapters on such topics as Förster resonance energy transfer (fret) with fluctuation algorithms, protein corona on nanoparticles by FCS, and FFS approaches to the study of receptors in live cells.
1. Cover image 2. Title page 3. Table of Contents 4. Copyright 5. Contributors 6. Preface 7. Methods in Enzymology 8. Chapter One. FCS in STED Microscopy 9. Chapter Two. Analyzing Förster Resonance Energy Transfer with Fluctuation Algorithms 10. Chapter Three. Fluorescence Fluctuation Spectroscopy Approaches to the Study of Receptors in Live Cells 11. Chapter Four. Studying the Protein Corona on Nanoparticles by FCS 12. Chapter Five. Studying Antibody–Antigen Interactions with Fluorescence Fluctuation Spectroscopy 13. Chapter Six. Fluorescence Fluctuation Approaches to the Study of Adhesion and Signaling 14. Chapter Seven. Interactions in Gene Expression Networks Studied by Two-Photon Fluorescence Fluctuation Spectroscopy 15. Chapter Eight. Studying Ion Exchange in Solution and at Biological Membranes by FCS 16. Chapter Nine. Fluctuation Analysis of Activity Biosensor Images for the Study of Information Flow in Signaling Pathways 17. Chapter Ten. Probing the Plasma Membrane Organization in Living Cells by Spot Variation Fluorescence Correlation Spectroscopy 18. Author Index 19. Subject Index
This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers fluorescence fluctuation spectroscopy and includes chapters on such topics as Förster resonance energy transfer (fret) with fluctuation algorithms, protein corona on nanoparticles by FCS, and FFS approaches to the study of receptors in live cells.
1. Cover image 2. Title page 3. Table of Contents 4. Copyright 5. Contributors 6. Preface 7. Methods in Enzymology 8. Chapter One. FCS in STED Microscopy 9. Chapter Two. Analyzing Förster Resonance Energy Transfer with Fluctuation Algorithms 10. Chapter Three. Fluorescence Fluctuation Spectroscopy Approaches to the Study of Receptors in Live Cells 11. Chapter Four. Studying the Protein Corona on Nanoparticles by FCS 12. Chapter Five. Studying Antibody–Antigen Interactions with Fluorescence Fluctuation Spectroscopy 13. Chapter Six. Fluorescence Fluctuation Approaches to the Study of Adhesion and Signaling 14. Chapter Seven. Interactions in Gene Expression Networks Studied by Two-Photon Fluorescence Fluctuation Spectroscopy 15. Chapter Eight. Studying Ion Exchange in Solution and at Biological Membranes by FCS 16. Chapter Nine. Fluctuation Analysis of Activity Biosensor Images for the Study of Information Flow in Signaling Pathways 17. Chapter Ten. Probing the Plasma Membrane Organization in Living Cells by Spot Variation Fluorescence Correlation Spectroscopy 18. Author Index 19. Subject Index
