The purpose of this work is to familiarize neuroscientists with the available tools for proteome research and their relative abilities and limitations. To know the identities of the thousands of different proteins in a cell, and the modifications to these proteins, along with how the amounts of both of these change in different conditions would revolutionize biology and medicine. While important strides are being made towards achieving the goal of global mRNA analysis, mRNA is not the functional endpoint of gene expression and mRNA expression may not directly equate with protein expression. There are many potential applications for proteomics in neuroscience: determination of the neuro-proteome, comparative protein expression profiling, post-translational protein modification profiling and mapping protein-protein interactions, to name but a few. Functional Genomics and Proteomics in Clinical Neuroscience will comment on all of these applications, but with an emphasis on protein expression profiling. This book combines the basic methodology of genomics and proteomics with the current applications of such technologies in understanding psychiatric illnesses.
* Introduction of basic methodologies in genomics and proteomics and their integration in psychiatry
* Development of the text in sections related to methods, application and future directions of these rapidly advancing technologies
* Use of actual data to illustrate many principles of functional genomics and proteomics.
* Introduction to bioinformatics and database management techniques
1. Cover image 2. Title page 3. Table of Contents 4. List of Contributors 5. Foreword 6. Section I Methodologies 7. Tissue preparation and banking 8. Functional genomic methodologies 9. Methods for proteomics in neuroscience 10. Functional genomics and proteomics in the clinical neurosciences: data mining and bioinformatics 11. Reproducibility of microarray studies: concordance of current analysis methods 12. Section II Applications of Genomics and Proteomic Technologies to Clinical Neuroscience 13. The genomics of mood disorders 14. Transcriptome alterations in schizophrenia: disturbing the functional architecture of the dorsolateral prefrontal cortex 15. Strategies for improving sensitivity of gene expression profiling: regulation of apoptosis in the limbic lobe of schizophrenics and bipolars 16. Assessment of genome and proteome profiles in cocaine abuse 17. Neuronal gene expression profiling: uncovering the molecular biology of neurodegenerative disease 18. Epileptogenesis-related genes revisited 19. Functional genomics of sex hormone-dependent neuroendocrine systems: specific and generalized actions in the CNS 20. Section III Future Directions 21. Implications for the practice of psychiatry 22. Human brain evolution 23. Subject Index 24. Erratum to Progress in Brain Research Vol. 158 Functional Genomics and Proteomics in the Clinical Neurosciences Scott E. Hemby and Sabine Bahn
The purpose of this work is to familiarize neuroscientists with the available tools for proteome research and their relative abilities and limitations. To know the identities of the thousands of different proteins in a cell, and the modifications to these proteins, along with how the amounts of both of these change in different conditions would revolutionize biology and medicine. While important strides are being made towards achieving the goal of global mRNA analysis, mRNA is not the functional endpoint of gene expression and mRNA expression may not directly equate with protein expression. There are many potential applications for proteomics in neuroscience: determination of the neuro-proteome, comparative protein expression profiling, post-translational protein modification profiling and mapping protein-protein interactions, to name but a few. Functional Genomics and Proteomics in Clinical Neuroscience will comment on all of these applications, but with an emphasis on protein expression profiling. This book combines the basic methodology of genomics and proteomics with the current applications of such technologies in understanding psychiatric illnesses.
* Introduction of basic methodologies in genomics and proteomics and their integration in psychiatry
* Development of the text in sections related to methods, application and future directions of these rapidly advancing technologies
* Use of actual data to illustrate many principles of functional genomics and proteomics.
* Introduction to bioinformatics and database management techniques
1. Cover image 2. Title page 3. Table of Contents 4. List of Contributors 5. Foreword 6. Section I Methodologies 7. Tissue preparation and banking 8. Functional genomic methodologies 9. Methods for proteomics in neuroscience 10. Functional genomics and proteomics in the clinical neurosciences: data mining and bioinformatics 11. Reproducibility of microarray studies: concordance of current analysis methods 12. Section II Applications of Genomics and Proteomic Technologies to Clinical Neuroscience 13. The genomics of mood disorders 14. Transcriptome alterations in schizophrenia: disturbing the functional architecture of the dorsolateral prefrontal cortex 15. Strategies for improving sensitivity of gene expression profiling: regulation of apoptosis in the limbic lobe of schizophrenics and bipolars 16. Assessment of genome and proteome profiles in cocaine abuse 17. Neuronal gene expression profiling: uncovering the molecular biology of neurodegenerative disease 18. Epileptogenesis-related genes revisited 19. Functional genomics of sex hormone-dependent neuroendocrine systems: specific and generalized actions in the CNS 20. Section III Future Directions 21. Implications for the practice of psychiatry 22. Human brain evolution 23. Subject Index 24. Erratum to Progress in Brain Research Vol. 158 Functional Genomics and Proteomics in the Clinical Neurosciences Scott E. Hemby and Sabine Bahn
