Immuno-oncology and immunotherapy, Part C, Volume 191 in the Methods in Cell Biology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of timely topics, including Extraction and quantification of histones from human cells, Expression and characterization of Phosphatidylserine-targeting antibodies for biochemical and therapeutic applications, ILC differentiation from HSCs in vitro, Methods to expand human Treg cells and assay their function, Monitoring rapid activation of human gamma/delta T cells by multicolor flow cytometry, Methods to induce T cell exhaustion in vitro, Ex vivo assessment of human neutrophil motility and migration, and much more.
Additional chapters focus on Flow cytometry-based monitoring of myeloid-derived suppressor cells in the peripheral blood of patients with solid tumors, Deciphering human blood and tumor neutrophil heterogeneity: Methods for isolation and assessing suppression of T-cell proliferation, Splenocyte anticancer citotoxicity assessment after prophylactic vaccination or drug treatment of tumor-bearing mice, Therapeutic treatment of tumor-bearing mice with drug-killed cancer cells: a method to confirm immunogenic cell death and assess its therapeutic effectiveness, and much more.
1. Immuno-Oncology and Immunotherapy - Part C - p. i 2. Cover image - p. i 3. Title page - p. i 4. Table of Contents - p. i 5. Series Page - p. i 6. Copyright - p. iv 7. Contributors - p. xi 8. Chapter One Simplified acid extraction and quantification of histones in human tumor cells - p. 1 9. Chapter Two Expression, purification and characterization of phosphatidylserine-targeting antibodies for biochemical and therapeutic applications - p. 15 10. Chapter Three In vitro ILC differentiation from human HSCs - p. 42 11. Chapter Four Optimizing protocols for human regulatory T isolation, expansion, and characterization - p. 60 12. Chapter Five Specific selection of stimulation-responsive γδ T-cells utilizing a short-term activation assay - p. 80 13. Chapter Six Generation of human and murine exhausted CD8+ T cells in vitro - p. 94 14. Chapter Seven Ex vivo assessment of human neutrophil motility and migration - p. 115 15. Chapter Eight Flow cytometry-based monitoring of myeloid-derived suppressor cells in the peripheral blood of patients with solid tumors - p. 135 16. Chapter Nine Deciphering neutrophil heterogeneity in human blood and tumors: Methods for isolating neutrophils and assessing their effect on T-cell proliferation - p. 151 17. Chapter Ten Splenocytes antitumor cytotoxicity assessment after prophylactic vaccination or drug treatment of tumor-bearing mice - p. 197 18. Chapter Eleven Use of drug-killed cancer cells: A method to assess the therapeutic effectiveness of immunogenic cell death - p. 212 19. Chapter Twelve Single-cell RNA flow cytometry to assess intratumoral production of cytokines/chemokines - p. 222 20. Chapter Thirteen Multidimensional profiling of cancer microenvironments in FFPE tissues by imaging mass cytometry - p. 248 21. Chapter Fourteen Evaluation of lymphocyte infiltration into cancer spheroids by immunofluorescent staining and 3D imaging - p. 270 22. Chapter Fifteen Quantitative pre-clinical imaging of hypoxia and vascularity using MRI and PET - p. 289 23. Chapter Sixteen Retrovirus-based manufacturing of chimeric antigen receptor-modified T cells for cancer therapy research - p. 329
Immuno-oncology and immunotherapy, Part C, Volume 191 in the Methods in Cell Biology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of timely topics, including Extraction and quantification of histones from human cells, Expression and characterization of Phosphatidylserine-targeting antibodies for biochemical and therapeutic applications, ILC differentiation from HSCs in vitro, Methods to expand human Treg cells and assay their function, Monitoring rapid activation of human gamma/delta T cells by multicolor flow cytometry, Methods to induce T cell exhaustion in vitro, Ex vivo assessment of human neutrophil motility and migration, and much more.
Additional chapters focus on Flow cytometry-based monitoring of myeloid-derived suppressor cells in the peripheral blood of patients with solid tumors, Deciphering human blood and tumor neutrophil heterogeneity: Methods for isolation and assessing suppression of T-cell proliferation, Splenocyte anticancer citotoxicity assessment after prophylactic vaccination or drug treatment of tumor-bearing mice, Therapeutic treatment of tumor-bearing mice with drug-killed cancer cells: a method to confirm immunogenic cell death and assess its therapeutic effectiveness, and much more.
1. Immuno-Oncology and Immunotherapy - Part C - p. i 2. Cover image - p. i 3. Title page - p. i 4. Table of Contents - p. i 5. Series Page - p. i 6. Copyright - p. iv 7. Contributors - p. xi 8. Chapter One Simplified acid extraction and quantification of histones in human tumor cells - p. 1 9. Chapter Two Expression, purification and characterization of phosphatidylserine-targeting antibodies for biochemical and therapeutic applications - p. 15 10. Chapter Three In vitro ILC differentiation from human HSCs - p. 42 11. Chapter Four Optimizing protocols for human regulatory T isolation, expansion, and characterization - p. 60 12. Chapter Five Specific selection of stimulation-responsive γδ T-cells utilizing a short-term activation assay - p. 80 13. Chapter Six Generation of human and murine exhausted CD8+ T cells in vitro - p. 94 14. Chapter Seven Ex vivo assessment of human neutrophil motility and migration - p. 115 15. Chapter Eight Flow cytometry-based monitoring of myeloid-derived suppressor cells in the peripheral blood of patients with solid tumors - p. 135 16. Chapter Nine Deciphering neutrophil heterogeneity in human blood and tumors: Methods for isolating neutrophils and assessing their effect on T-cell proliferation - p. 151 17. Chapter Ten Splenocytes antitumor cytotoxicity assessment after prophylactic vaccination or drug treatment of tumor-bearing mice - p. 197 18. Chapter Eleven Use of drug-killed cancer cells: A method to assess the therapeutic effectiveness of immunogenic cell death - p. 212 19. Chapter Twelve Single-cell RNA flow cytometry to assess intratumoral production of cytokines/chemokines - p. 222 20. Chapter Thirteen Multidimensional profiling of cancer microenvironments in FFPE tissues by imaging mass cytometry - p. 248 21. Chapter Fourteen Evaluation of lymphocyte infiltration into cancer spheroids by immunofluorescent staining and 3D imaging - p. 270 22. Chapter Fifteen Quantitative pre-clinical imaging of hypoxia and vascularity using MRI and PET - p. 289 23. Chapter Sixteen Retrovirus-based manufacturing of chimeric antigen receptor-modified T cells for cancer therapy research - p. 329
