Immuno-oncology and immunotherapy, Part D, Volume 196 in the Methods in Cell Biology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of timely topics, including Investigating the endothelial-immune axis in cancer, Establishing Tyro3, Axl, and Mertk Chinese Hamster Ovary (CHO) Reporter Cell Lines for Cancer Immunology and Therapeutic Applications, Amino acid analog-based labelling of the immunomodulatory cancer secretome, Human and mouse iNKT cell expansion and engineering with viral vectors, Next-generation deconvolution of the tumor microenvironment with omnideconv, and much more.
Additional sections cover Comprehensive prediction of tumor neoantigens with NextNEOpi, Ex vivo profiling of organotypic tumor spheroids in 3D microfluidic culture, A flow cytometry-based assay for evaluation of T cell activation and degranulation in coculture systems, An adenosine production assay suitable for 2D and 3D cancer cell culture, Determination of lactate in the tumor interstitial fluid, Combined in vitro differentiation and cell sorting-based isolation of highly pure mouse bone marrow-derived basophils, Organ-on-Chip immunostaining method for three-dimensional identification and study of immune cells responding to drug-treated tumor cells, and much more.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Series Page - p. iii 5. Copyright - p. iv 6. Contributors - p. xi 7. Chapter One: Guidelines for the assessment of high endothelial venule functionality and health - p. 1 8. Chapter Two: Establishing Tyro3, Axl, and Mertk Chinese hamster ovary (CHO) reporter cell lines for cancer immunology and therapeutic applications - p. 17 9. Chapter Three: Assessment of the primary cancer cell secretome using amino acid-analog labeling - p. 43 10. Chapter Four: Human and mouse iNKT cell expansion and engineering with viral vectors - p. 67 11. Chapter Five: Next-generation deconvolution of the tumor microenvironment with omnideconv - p. 87 12. Chapter Six: Comprehensive prediction of tumor neoantigens with nextNEOpi - p. 113 13. Chapter Seven: Preparation and analysis of monotypic and organotypic tumor spheroids - p. 139 14. Chapter Eight: Evaluating functional anti-tumor T cell responses in autologous 2D co-cultures using flow cytometry - p. 161 15. Chapter Nine: Relative adenosine production assay suitable for 2D and 3D tumor cell culture - p. 171 16. Chapter Ten: Isolation of tumor interstitial fluid for metabolic determinations - p. 177 17. Chapter Eleven: Combined in vitro differentiation and cell sorting-based isolation of highly pure mouse bone marrow-derived basophils - p. 193 18. Chapter Twelve: Organ-on-chip immunostaining method for three-dimensional identification and study of immune cells responding to drug-treated tumor cells - p. 209 19. Chapter Thirteen: Evaluating transcriptional tumour microenvironment response to immunotherapy using microarray analysis: From harvesting tumours to data analysis - p. 225 20. Chapter Fourteen: Expansion and activation of NK cells supported by accessory cells. Phenotypic and functional characterization - p. 237 21. Chapter Fifteen: Matrigel implants embedded with IDVCs (IDO1-dependent vascularizing cells) to study inflammatory neovascularization - p. 251 22. Chapter Sixteen: Phenotypic assessment of dendritic cell maturation by cost-effective custom ELISA assays - p. 271
Immuno-oncology and immunotherapy, Part D, Volume 196 in the Methods in Cell Biology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of timely topics, including Investigating the endothelial-immune axis in cancer, Establishing Tyro3, Axl, and Mertk Chinese Hamster Ovary (CHO) Reporter Cell Lines for Cancer Immunology and Therapeutic Applications, Amino acid analog-based labelling of the immunomodulatory cancer secretome, Human and mouse iNKT cell expansion and engineering with viral vectors, Next-generation deconvolution of the tumor microenvironment with omnideconv, and much more.
Additional sections cover Comprehensive prediction of tumor neoantigens with NextNEOpi, Ex vivo profiling of organotypic tumor spheroids in 3D microfluidic culture, A flow cytometry-based assay for evaluation of T cell activation and degranulation in coculture systems, An adenosine production assay suitable for 2D and 3D cancer cell culture, Determination of lactate in the tumor interstitial fluid, Combined in vitro differentiation and cell sorting-based isolation of highly pure mouse bone marrow-derived basophils, Organ-on-Chip immunostaining method for three-dimensional identification and study of immune cells responding to drug-treated tumor cells, and much more.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Series Page - p. iii 5. Copyright - p. iv 6. Contributors - p. xi 7. Chapter One: Guidelines for the assessment of high endothelial venule functionality and health - p. 1 8. Chapter Two: Establishing Tyro3, Axl, and Mertk Chinese hamster ovary (CHO) reporter cell lines for cancer immunology and therapeutic applications - p. 17 9. Chapter Three: Assessment of the primary cancer cell secretome using amino acid-analog labeling - p. 43 10. Chapter Four: Human and mouse iNKT cell expansion and engineering with viral vectors - p. 67 11. Chapter Five: Next-generation deconvolution of the tumor microenvironment with omnideconv - p. 87 12. Chapter Six: Comprehensive prediction of tumor neoantigens with nextNEOpi - p. 113 13. Chapter Seven: Preparation and analysis of monotypic and organotypic tumor spheroids - p. 139 14. Chapter Eight: Evaluating functional anti-tumor T cell responses in autologous 2D co-cultures using flow cytometry - p. 161 15. Chapter Nine: Relative adenosine production assay suitable for 2D and 3D tumor cell culture - p. 171 16. Chapter Ten: Isolation of tumor interstitial fluid for metabolic determinations - p. 177 17. Chapter Eleven: Combined in vitro differentiation and cell sorting-based isolation of highly pure mouse bone marrow-derived basophils - p. 193 18. Chapter Twelve: Organ-on-chip immunostaining method for three-dimensional identification and study of immune cells responding to drug-treated tumor cells - p. 209 19. Chapter Thirteen: Evaluating transcriptional tumour microenvironment response to immunotherapy using microarray analysis: From harvesting tumours to data analysis - p. 225 20. Chapter Fourteen: Expansion and activation of NK cells supported by accessory cells. Phenotypic and functional characterization - p. 237 21. Chapter Fifteen: Matrigel implants embedded with IDVCs (IDO1-dependent vascularizing cells) to study inflammatory neovascularization - p. 251 22. Chapter Sixteen: Phenotypic assessment of dendritic cell maturation by cost-effective custom ELISA assays - p. 271
