Immuno-oncology and immunotherapy, Part E, Volume 199 in the Methods in Cell Biology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of timely topics, including an Evaluation of agents that affect anti-tumor function of CD8+ T cells at activation, Cancer cyro-immunotherapy: Models and methods, Methods for analysis of cancer- and TLS-associated immune infiltrates by multiplex immunofluorescence histology, In-vivo measurement of the extracellular ATP concentration by bio-luminescent probes, and much more.
Additional sections cover the Analysis of the tumor microenvironment using imaging mass cytometry data, Saphenous vein blood collection for different immune analyses of living mice, Use of mouse KO models to validate the specificity of monoclonal antibodies, Imaging mass cytometry to analyze the immune TME, Autoimmune arthritis: Transgenic mouse models and methods, Assessment of human and mouse tumor-antigen specific CD8+ T cells by multimer staining in multiple compartments, and more.
1. Title of Book - p. i 2. Cover image - p. i 3. Title page - p. ii 4. Table of Contents - p. iii 5. Copyright - p. iv 6. Contributors - p. xi 7. Chapter One: Evaluation of agents that affect anti-tumor function of CD8 + T cells when employed at the time of T-cell activation - p. 1 8. Chapter Two: Tumor cryoablation: Preclinical models and methods to explore immunological effects - p. 23 9. Chapter Three: Methods for analysis of tertiary lymphoid structures and immune activity by multiplex immunofluorescence histology - p. 39 10. Chapter Four: In-vivo measurement of the extracellular ATP concentration by bio-luminescence: The pmeLUC probe - p. 53 11. Chapter Five: Analysis of the tumor microenvironment using imaging mass cytometry data - p. 67 12. Chapter Six: Saphenous vein blood collection for different immune analyses of living mice - p. 95 13. Chapter Seven: Use of mouse knockout models to validate the specificity of monoclonal antibodies - p. 103 14. Chapter Eight: Imaging mass cytometry-derived single cell extraction, transformation and phenotyping to analyze immune tumor microenvironments - p. 117 15. Chapter Nine: Autoimmune arthritis: Transgenic mouse models and methods - p. 133 16. Chapter Ten: Assessment of human and mouse tumor-antigen specific CD8 + T cells by multimer staining in multiple compartments - p. 151 17. Chapter Eleven: Generation and functional evaluation of bispecific T cell engaging antibodies - p. 167 18. Chapter Twelve: Isolation and phenotype analysis of tissue-resident lymphocytes from mouse colon - p. 187 19. Chapter Thirteen: Analysis of single-cell TCR repertoires and gene expression from multi-modal scRNA-seq data - p. 201 20. Chapter Fourteen: Analysis of the tumor immune microenvironment of irradiated brain tumors by flow cytometry - p. 231 21. Chapter Fifteen: Assessment of in vitro interactions between eosinophils and cancer cells by imaging-enhanced flow cytometry and time lapse microscopy - p. 245 22. Chapter Sixteen: Pharmacological screening to identify potential regulators of antigen presentation by dendritic cells - p. 271
Immuno-oncology and immunotherapy, Part E, Volume 199 in the Methods in Cell Biology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of timely topics, including an Evaluation of agents that affect anti-tumor function of CD8+ T cells at activation, Cancer cyro-immunotherapy: Models and methods, Methods for analysis of cancer- and TLS-associated immune infiltrates by multiplex immunofluorescence histology, In-vivo measurement of the extracellular ATP concentration by bio-luminescent probes, and much more.
Additional sections cover the Analysis of the tumor microenvironment using imaging mass cytometry data, Saphenous vein blood collection for different immune analyses of living mice, Use of mouse KO models to validate the specificity of monoclonal antibodies, Imaging mass cytometry to analyze the immune TME, Autoimmune arthritis: Transgenic mouse models and methods, Assessment of human and mouse tumor-antigen specific CD8+ T cells by multimer staining in multiple compartments, and more.
1. Title of Book - p. i 2. Cover image - p. i 3. Title page - p. ii 4. Table of Contents - p. iii 5. Copyright - p. iv 6. Contributors - p. xi 7. Chapter One: Evaluation of agents that affect anti-tumor function of CD8 + T cells when employed at the time of T-cell activation - p. 1 8. Chapter Two: Tumor cryoablation: Preclinical models and methods to explore immunological effects - p. 23 9. Chapter Three: Methods for analysis of tertiary lymphoid structures and immune activity by multiplex immunofluorescence histology - p. 39 10. Chapter Four: In-vivo measurement of the extracellular ATP concentration by bio-luminescence: The pmeLUC probe - p. 53 11. Chapter Five: Analysis of the tumor microenvironment using imaging mass cytometry data - p. 67 12. Chapter Six: Saphenous vein blood collection for different immune analyses of living mice - p. 95 13. Chapter Seven: Use of mouse knockout models to validate the specificity of monoclonal antibodies - p. 103 14. Chapter Eight: Imaging mass cytometry-derived single cell extraction, transformation and phenotyping to analyze immune tumor microenvironments - p. 117 15. Chapter Nine: Autoimmune arthritis: Transgenic mouse models and methods - p. 133 16. Chapter Ten: Assessment of human and mouse tumor-antigen specific CD8 + T cells by multimer staining in multiple compartments - p. 151 17. Chapter Eleven: Generation and functional evaluation of bispecific T cell engaging antibodies - p. 167 18. Chapter Twelve: Isolation and phenotype analysis of tissue-resident lymphocytes from mouse colon - p. 187 19. Chapter Thirteen: Analysis of single-cell TCR repertoires and gene expression from multi-modal scRNA-seq data - p. 201 20. Chapter Fourteen: Analysis of the tumor immune microenvironment of irradiated brain tumors by flow cytometry - p. 231 21. Chapter Fifteen: Assessment of in vitro interactions between eosinophils and cancer cells by imaging-enhanced flow cytometry and time lapse microscopy - p. 245 22. Chapter Sixteen: Pharmacological screening to identify potential regulators of antigen presentation by dendritic cells - p. 271
