Integrated Methods in Protein Biochemistry: Part A, Volume 677, the latest release in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on topics such as DNA and protein engineering to create protein bioswitches with new functions, Interaction and cross-talk of prelamin A with integral membrane zinc metalloproteases, An experimental protocol to study lipid transfer proteins, Synthesis of small heat shock proteins, Druggable p-p interacting sites for Co-chaperone DNAJA1 and its partner proteins, An experimental protocol for glycoconjugate analysis, Methods for proximity-based biotinylation combined with Mass Spectrometry, and more.
Additional chapters cover Synthetic antibody fragments as conformational sensors of protein activation and trafficking, Expression, purification, functional analysis and crystallization of Rag GTPase, Purification of bacterial transcription elongation complexes by photoreversible immobilization, Inhibition of c-Myc-MAX heterodimerization, Fluorogenic RNA aptamers to probe transcription by multi-subunit RNA polymerases, and much more.
1. Cover image - p. i 2. Table of Contents - p. iii 3. Series Page - p. iii 4. Copyright - p. iii 5. Contributors - p. iii 6. Preface - p. iii 7. Chapter One: Engineering protein and DNA tools for creating DNA-dependent protein switches - p. 4 8. Chapter Two: Reconstitution and biochemical studies of extended synaptotagmin-mediated lipid transport - p. 33 9. Chapter Three: Synthesis of O-GlcNAcylated small heat shock proteins - p. 63 10. Chapter Four: Druggable sites/pockets of the p53-DNAJA1 protein–protein interaction: In silico modeling and in vitro/in vivo validation - p. 83 11. Chapter Five: RAPIDS, a method for sub-compartmental identification of protein interactomes - p. 109 12. Chapter Six: Purification and biochemical characterization of the Rag GTPase heterodimer - p. 131 13. Chapter Seven: Isolation of synchronized E. coli elongation complexes for solid-phase and solution-based in vitro transcription assays - p. 159 14. Chapter Eight: Cellular experiments to study the inhibition of c-Myc/MAX heterodimerization - p. 193 15. Chapter Nine: Fluorogenic RNA aptamers to probe transcription initiation and co-transcriptional RNA folding by multi-subunit RNA polymerases - p. 207 16. Chapter Ten: Phosphorylation and subcellular localization of human phospholipase A1, DDHD1/PA-PLA1 - p. 235 17. Chapter Eleven: Elucidation of folding pathways of knotted proteins - p. 275 18. Chapter Twelve: Biophysical and structural characterizations of the effects of mutations on the structure–activity relationships of SARS-CoV-2 spike protein - p. 299 19. Chapter Thirteen: A gel electrophoresis-based assay for measuring enzymatic RNA decapping activity - p. 328 20. Chapter Fourteen: Nanoluciferase-based cell fusion assay for rapid and high-throughput assessment of SARS-CoV-2-neutralizing antibodies in patient samples - p. 351 21. Chapter Fifteen: Linchpin-directed precise labeling of lysine in native proteins, purification, and analysis - p. 383 22. Chapter Sixteen: In vitro and in cellulae methods for determining the target protein SUMOylation - p. 397 23. Chapter Seventeen: Two-dimensional electrophoresis–cellular thermal shift assay (2DE-CETSA) for target identification of bioactive compounds - p. 425
Integrated Methods in Protein Biochemistry: Part A, Volume 677, the latest release in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on topics such as DNA and protein engineering to create protein bioswitches with new functions, Interaction and cross-talk of prelamin A with integral membrane zinc metalloproteases, An experimental protocol to study lipid transfer proteins, Synthesis of small heat shock proteins, Druggable p-p interacting sites for Co-chaperone DNAJA1 and its partner proteins, An experimental protocol for glycoconjugate analysis, Methods for proximity-based biotinylation combined with Mass Spectrometry, and more.
Additional chapters cover Synthetic antibody fragments as conformational sensors of protein activation and trafficking, Expression, purification, functional analysis and crystallization of Rag GTPase, Purification of bacterial transcription elongation complexes by photoreversible immobilization, Inhibition of c-Myc-MAX heterodimerization, Fluorogenic RNA aptamers to probe transcription by multi-subunit RNA polymerases, and much more.
1. Cover image - p. i 2. Table of Contents - p. iii 3. Series Page - p. iii 4. Copyright - p. iii 5. Contributors - p. iii 6. Preface - p. iii 7. Chapter One: Engineering protein and DNA tools for creating DNA-dependent protein switches - p. 4 8. Chapter Two: Reconstitution and biochemical studies of extended synaptotagmin-mediated lipid transport - p. 33 9. Chapter Three: Synthesis of O-GlcNAcylated small heat shock proteins - p. 63 10. Chapter Four: Druggable sites/pockets of the p53-DNAJA1 protein–protein interaction: In silico modeling and in vitro/in vivo validation - p. 83 11. Chapter Five: RAPIDS, a method for sub-compartmental identification of protein interactomes - p. 109 12. Chapter Six: Purification and biochemical characterization of the Rag GTPase heterodimer - p. 131 13. Chapter Seven: Isolation of synchronized E. coli elongation complexes for solid-phase and solution-based in vitro transcription assays - p. 159 14. Chapter Eight: Cellular experiments to study the inhibition of c-Myc/MAX heterodimerization - p. 193 15. Chapter Nine: Fluorogenic RNA aptamers to probe transcription initiation and co-transcriptional RNA folding by multi-subunit RNA polymerases - p. 207 16. Chapter Ten: Phosphorylation and subcellular localization of human phospholipase A1, DDHD1/PA-PLA1 - p. 235 17. Chapter Eleven: Elucidation of folding pathways of knotted proteins - p. 275 18. Chapter Twelve: Biophysical and structural characterizations of the effects of mutations on the structure–activity relationships of SARS-CoV-2 spike protein - p. 299 19. Chapter Thirteen: A gel electrophoresis-based assay for measuring enzymatic RNA decapping activity - p. 328 20. Chapter Fourteen: Nanoluciferase-based cell fusion assay for rapid and high-throughput assessment of SARS-CoV-2-neutralizing antibodies in patient samples - p. 351 21. Chapter Fifteen: Linchpin-directed precise labeling of lysine in native proteins, purification, and analysis - p. 383 22. Chapter Sixteen: In vitro and in cellulae methods for determining the target protein SUMOylation - p. 397 23. Chapter Seventeen: Two-dimensional electrophoresis–cellular thermal shift assay (2DE-CETSA) for target identification of bioactive compounds - p. 425
