Fish, and particularly zebrafish, have become the fastest-growing segment of the research population. They offer several advantages, in terms of biology and technologies to apply, and thus are employed in numerous research fields. Laboratory Fish in Biomedical Research: Biology, Husbandry and Research Applications for Zebrafish, Medaka, Killifish, Swordtail Fish, Cavefish, Stickleback, Goldfish and Danionella Translucida addresses the relevant and increasing need to collect cutting-edge knowledge on husbandry, maintenance, welfare and experimental protocols of the most common freshwater species under standard laboratory conditions.
1. Cover image - p. Cover 2. Title page - p. ii 3. Table of Contents - p. iii 4. Copyright - p. iii 5. Contributors - p. iii 6. Fish as model systems - p. iii 7. List of Tables - p. xxiii 8. Section 1: Zebrafish (Danio rerio) - p. xxiii 9. Chapter 1: Biology and research applications - p. xxiii 10. Chapter 2: Housing and maintenance of zebrafish, new technologies in laboratory aquatic systems and considerations for facility design - p. 16 11. Chapter 3: Breeding and larviculture of zebrafish (Danio rerio) - p. 57 12. Chapter 4: Health monitoring, disease, and clinical pathology - p. 78 13. Chapter 5: The welfare of zebrafish - p. 98 14. Chapter 6: Analgesia, anesthesia, and euthanasia in zebrafish - p. 113 15. Chapter 7: Transgenesis, mutagenesis, knockdown, and genetic colony management - p. 133 16. Chapter 8: Sperm cryopreservation, in vitro fertilization, and embryo freezing - p. 151 17. Section 2: Other small freshwater fish - p. 177 18. Chapter 9: Medaka as a model teleost: characteristics and approaches of genetic modification - p. 177 19. Chapter 10: Integrated analyses using medaka as a powerful model animal toward understanding various aspects of reproductive regulation - p. 208 20. Chapter 11: The African turquoise killifish (Nothobranchius furzeri): biology and research applications - p. 237 21. Chapter 12: Challenges in keeping annual killifish - p. 278 22. Chapter 13: Mexican tetra (Astyanax mexicanus): biology, husbandry, and experimental protocols - p. 306 23. Chapter 14: The housing, care, and use of a laboratory three-spined stickleback colony - p. 340 24. Chapter 15: Goldfish (Carassius auratus): biology, husbandry, and research applications - p. 368 25. Chapter 16: Danionella translucida, a tankful of new opportunities - p. 400 26. Section 3: Databases - p. 417 27. Chapter 17: Fish inventory databases - p. 417 28. Index - p. 433
Fish, and particularly zebrafish, have become the fastest-growing segment of the research population. They offer several advantages, in terms of biology and technologies to apply, and thus are employed in numerous research fields. Laboratory Fish in Biomedical Research: Biology, Husbandry and Research Applications for Zebrafish, Medaka, Killifish, Swordtail Fish, Cavefish, Stickleback, Goldfish and Danionella Translucida addresses the relevant and increasing need to collect cutting-edge knowledge on husbandry, maintenance, welfare and experimental protocols of the most common freshwater species under standard laboratory conditions.
1. Cover image - p. Cover 2. Title page - p. ii 3. Table of Contents - p. iii 4. Copyright - p. iii 5. Contributors - p. iii 6. Fish as model systems - p. iii 7. List of Tables - p. xxiii 8. Section 1: Zebrafish (Danio rerio) - p. xxiii 9. Chapter 1: Biology and research applications - p. xxiii 10. Chapter 2: Housing and maintenance of zebrafish, new technologies in laboratory aquatic systems and considerations for facility design - p. 16 11. Chapter 3: Breeding and larviculture of zebrafish (Danio rerio) - p. 57 12. Chapter 4: Health monitoring, disease, and clinical pathology - p. 78 13. Chapter 5: The welfare of zebrafish - p. 98 14. Chapter 6: Analgesia, anesthesia, and euthanasia in zebrafish - p. 113 15. Chapter 7: Transgenesis, mutagenesis, knockdown, and genetic colony management - p. 133 16. Chapter 8: Sperm cryopreservation, in vitro fertilization, and embryo freezing - p. 151 17. Section 2: Other small freshwater fish - p. 177 18. Chapter 9: Medaka as a model teleost: characteristics and approaches of genetic modification - p. 177 19. Chapter 10: Integrated analyses using medaka as a powerful model animal toward understanding various aspects of reproductive regulation - p. 208 20. Chapter 11: The African turquoise killifish (Nothobranchius furzeri): biology and research applications - p. 237 21. Chapter 12: Challenges in keeping annual killifish - p. 278 22. Chapter 13: Mexican tetra (Astyanax mexicanus): biology, husbandry, and experimental protocols - p. 306 23. Chapter 14: The housing, care, and use of a laboratory three-spined stickleback colony - p. 340 24. Chapter 15: Goldfish (Carassius auratus): biology, husbandry, and research applications - p. 368 25. Chapter 16: Danionella translucida, a tankful of new opportunities - p. 400 26. Section 3: Databases - p. 417 27. Chapter 17: Fish inventory databases - p. 417 28. Index - p. 433
