The emergence and re-emergence of new infectious diseases places emphasis on the need for greater investment in rapid healthcare diagnostic solutions. As a result, lateral flow immunoassay technology is quickly evolving with new inventions. Lateral Flow Assay for Rapid Point-of-Care Testing covers today’s most significant advancements, emerging trends, and future directions linked with this technology. The book begins with a review of the format, flow, and design of old-generation lateral flow assays and the challenges impeding performance before moving into innovation in materials for rapid test strips, advances in manipulating sample flow, and the latest developments in molecular imprinting and bioconjugation techniques. Subsequent sections cover various biorecognition elements and review signal amplification as well as quantification trends. Final chapters delve into recent applications for infectious diseases detection, monitoring antibiotic resistance, multiplex detection of analytes, as well as future directions leading to the next generation of healthcare. Academicians, researchers, industrialists, and device manufacturers linked to the development of rapid and affordable point-of-care immunodiagnostics will find value in this resource.
1. Title of Book - p. i 2. Cover image - p. i 3. Title page - p. ii 4. Table of Contents - p. iii 5. Copyright - p. iv 6. Contributors - p. xi 7. Preface - p. xiii 8. Section I. Lateral flow assay format, flow, and design - p. S1 9. Chapter 1. Lateral flow assays and their role in healthcare - p. 3 10. Chapter 2. Innovations in materials used for the manufacturing of rapid test strip - p. 19 11. Chapter 3. Advances in manipulating sample flow in LFA - p. 33 12. Chapter 4. Molecular imprinting and bioconjugation in lateral flow assays (LFAs) - p. 43 13. Section II. Biorecognition elements in lateral flow assays - p. S2 14. Chapter 5. Nucleic acids and aptamers - p. 61 15. Chapter 6. CRISPR-cas based lateral flow assays for diagnosis - p. 69 16. Chapter 7. Molecularly imprinted polymers - p. 89 17. Section III. Signal amplification and quantification - p. S3 18. Chapter 8. Fluorescent, upconverting, and SERS molecules - p. 107 19. Chapter 9. Lateral flow immunoassays (LFIA) with quantum dots as the reporter - p. 135 20. Chapter 10. Latest technology of signal acquisition-readers and devices - p. 147 21. Chapter 11. Integrated Data analysis with smartphones, internet, and AI - p. 161 22. Chapter 12. Visual semi-quantitation - p. 173 23. Section IV. Recent applications - p. S4 24. Chapter 13. Advancements in LFA for infectious diseases detection - p. 191 25. Chapter 14. LFA technology for monitoring antibiotic resistance - p. 209 26. Chapter 15. Multiplex lateral flow assay - p. 221 27. Chapter 16. Emerging trends and future directions in LFA - p. 227 28. Index - p. 237
The emergence and re-emergence of new infectious diseases places emphasis on the need for greater investment in rapid healthcare diagnostic solutions. As a result, lateral flow immunoassay technology is quickly evolving with new inventions. Lateral Flow Assay for Rapid Point-of-Care Testing covers today’s most significant advancements, emerging trends, and future directions linked with this technology. The book begins with a review of the format, flow, and design of old-generation lateral flow assays and the challenges impeding performance before moving into innovation in materials for rapid test strips, advances in manipulating sample flow, and the latest developments in molecular imprinting and bioconjugation techniques. Subsequent sections cover various biorecognition elements and review signal amplification as well as quantification trends. Final chapters delve into recent applications for infectious diseases detection, monitoring antibiotic resistance, multiplex detection of analytes, as well as future directions leading to the next generation of healthcare. Academicians, researchers, industrialists, and device manufacturers linked to the development of rapid and affordable point-of-care immunodiagnostics will find value in this resource.
1. Title of Book - p. i 2. Cover image - p. i 3. Title page - p. ii 4. Table of Contents - p. iii 5. Copyright - p. iv 6. Contributors - p. xi 7. Preface - p. xiii 8. Section I. Lateral flow assay format, flow, and design - p. S1 9. Chapter 1. Lateral flow assays and their role in healthcare - p. 3 10. Chapter 2. Innovations in materials used for the manufacturing of rapid test strip - p. 19 11. Chapter 3. Advances in manipulating sample flow in LFA - p. 33 12. Chapter 4. Molecular imprinting and bioconjugation in lateral flow assays (LFAs) - p. 43 13. Section II. Biorecognition elements in lateral flow assays - p. S2 14. Chapter 5. Nucleic acids and aptamers - p. 61 15. Chapter 6. CRISPR-cas based lateral flow assays for diagnosis - p. 69 16. Chapter 7. Molecularly imprinted polymers - p. 89 17. Section III. Signal amplification and quantification - p. S3 18. Chapter 8. Fluorescent, upconverting, and SERS molecules - p. 107 19. Chapter 9. Lateral flow immunoassays (LFIA) with quantum dots as the reporter - p. 135 20. Chapter 10. Latest technology of signal acquisition-readers and devices - p. 147 21. Chapter 11. Integrated Data analysis with smartphones, internet, and AI - p. 161 22. Chapter 12. Visual semi-quantitation - p. 173 23. Section IV. Recent applications - p. S4 24. Chapter 13. Advancements in LFA for infectious diseases detection - p. 191 25. Chapter 14. LFA technology for monitoring antibiotic resistance - p. 209 26. Chapter 15. Multiplex lateral flow assay - p. 221 27. Chapter 16. Emerging trends and future directions in LFA - p. 227 28. Index - p. 237
