Lignin-Degrading Enzymes volume 716 highlights new advances in the field, with this new volume presenting interesting chapters. Each chapter is written by an international board of authors.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Series Page - p. iii 5. Copyright - p. iv 6. Contributors - p. xv 7. Preface - p. xix 8. Chapter One: Analytical strategies for the investigation of enzymatic and microbial ligninolytic activities - p. 1 9. Chapter Two: NMR spectroscopy: An invaluable tool to identify lignin structural modifications induced by oxidative enzymes - p. 33 10. Chapter Three: Assay methods and colorimetric screens for lignin-degrading microbes and lignin-oxidising enzymes - p. 105 11. Chapter Four: Dye-decolorising peroxidase DyP1B from Pseudomonas fluorescens: Expression, reconstitution and reaction with polymeric lignin substrates - p. 125 12. Chapter Five: Expression and purification of bacterial dye-decolorizing peroxidases using Escherichia coli - p. 143 13. Chapter Six: Optimized protocols to measure stability of ligninolytic enzymes - p. 157 14. Chapter Seven: Recombinant expression of bacterial lignin-active laccases and peroxidase in Streptomyces lividans - p. 199 15. Chapter Eight: Design, construction and characterization of laccase-xylanase chimeras by insertional fusion - p. 217 16. Chapter Nine: β-Etherases in lignin valorization - p. 239 17. Chapter Ten: Glycolate oxidase from Rhodococcus jostii RHA1: An accessory enzyme for lignin bioconversion with bacterial DyP-type peroxidases - p. 263 18. Chapter Eleven: Characterization of bacterial transporters involved in the uptake of lignin-derived aromatic compounds - p. 285 19. Chapter Twelve: Strategies for lignocellulosic hydroxycinnamic acid release and biotransformation into high-value coniferyl alcohol using feruloyl esterase and double reduction whole cell biocatalyst - p. 313 20. Chapter Thirteen: Feruloyl-CoA synthetases and feruloyl-CoA hydratase/lyases: Expression, biochemical characterisation, and generation of vanillin from ferulic acid and lignocellulosic hydrolysates - p. 341 21. Chapter Fourteen: Ligninolytic peroxidases: A guide for their heterologous expression in E. coli and protocols to evaluate their activity on lignin - p. 361 22. Chapter Fifteen: Heterologous expression, in vitro refolding and steady-state kinetics of fungal aryl-alcohol oxidase - p. 381 23. Chapter Sixteen: Assessing iron reductase activity: Methodologies for fungal degradation of lignocellulose - p. 403
Lignin-Degrading Enzymes volume 716 highlights new advances in the field, with this new volume presenting interesting chapters. Each chapter is written by an international board of authors.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Series Page - p. iii 5. Copyright - p. iv 6. Contributors - p. xv 7. Preface - p. xix 8. Chapter One: Analytical strategies for the investigation of enzymatic and microbial ligninolytic activities - p. 1 9. Chapter Two: NMR spectroscopy: An invaluable tool to identify lignin structural modifications induced by oxidative enzymes - p. 33 10. Chapter Three: Assay methods and colorimetric screens for lignin-degrading microbes and lignin-oxidising enzymes - p. 105 11. Chapter Four: Dye-decolorising peroxidase DyP1B from Pseudomonas fluorescens: Expression, reconstitution and reaction with polymeric lignin substrates - p. 125 12. Chapter Five: Expression and purification of bacterial dye-decolorizing peroxidases using Escherichia coli - p. 143 13. Chapter Six: Optimized protocols to measure stability of ligninolytic enzymes - p. 157 14. Chapter Seven: Recombinant expression of bacterial lignin-active laccases and peroxidase in Streptomyces lividans - p. 199 15. Chapter Eight: Design, construction and characterization of laccase-xylanase chimeras by insertional fusion - p. 217 16. Chapter Nine: β-Etherases in lignin valorization - p. 239 17. Chapter Ten: Glycolate oxidase from Rhodococcus jostii RHA1: An accessory enzyme for lignin bioconversion with bacterial DyP-type peroxidases - p. 263 18. Chapter Eleven: Characterization of bacterial transporters involved in the uptake of lignin-derived aromatic compounds - p. 285 19. Chapter Twelve: Strategies for lignocellulosic hydroxycinnamic acid release and biotransformation into high-value coniferyl alcohol using feruloyl esterase and double reduction whole cell biocatalyst - p. 313 20. Chapter Thirteen: Feruloyl-CoA synthetases and feruloyl-CoA hydratase/lyases: Expression, biochemical characterisation, and generation of vanillin from ferulic acid and lignocellulosic hydrolysates - p. 341 21. Chapter Fourteen: Ligninolytic peroxidases: A guide for their heterologous expression in E. coli and protocols to evaluate their activity on lignin - p. 361 22. Chapter Fifteen: Heterologous expression, in vitro refolding and steady-state kinetics of fungal aryl-alcohol oxidase - p. 381 23. Chapter Sixteen: Assessing iron reductase activity: Methodologies for fungal degradation of lignocellulose - p. 403
