Low-Abundance Proteome Discovery addresses the most critical challenge in biomarker discovery and progress: the identification of low-abundance proteins. The book describes an original strategy developed by the authors that permits the detection of protein species typically found in very low abundance and that may yield valuable clues to future discoveries. Known as combinatorial peptide ligand libraries, these new methodologies are one of the hottest topics related to the study of proteomics and have applications in medical diagnostics, food quality, and plant analysis. The book is written for university and industry scientists starting proteomic studies of complex matrices (e.g., biological fluids, biopsies, recalcitrant plant tissues, foodstuff, and beverage analysis), researchers doing wet chemistry, and graduate-level students in the areas of analytical and biochemistry, biology, and genetics.
1. Cover image 2. Title page 3. Table of Contents 4. Copyright 5. Acknowledgments 6. Preface 7. Biography 8. Abbreviations 9. Trade Names 10. Antiphon 11. Chapter 1. Introducing Low-Abundance Species in Proteome Analysis 12. Chapter 2. Chromatographic and Electrophoretic Prefractionation Tools in Proteome Analysis 13. Chapter 3. Current Low-Abundance Protein Access 14. Chapter 4. Low-Abundance Protein Access by Combinatorial Peptide Libraries 15. 4.1 Combinatorial Chemistry 16. 4.2 Combinatorial Peptide Libraries 17. 4.3 Preparation of Solid-phase Combinatorial Peptide Ligand Libraries 18. 4.4 Use of Peptide Ligand Libraries as a Means of Reducing Protein Dynamic Range Concentration 19. 4.5 Peptide Ligand Library Properties in Protein Capturing 20. 4.6 Elution Mechanism Diversity and Complexity 21. 4.7 Selection of Elution Processes as a Function of Analytical Methods 22. 4.8 Towards a two-dimensional protein capture? 23. 4.9 References 24. Chapter 5. Plant Proteomics and Food and Beverage Analysis via CPLL Capture 25. Chapter 6. Biomedical Involvements of Low-Abundance Proteins 26. Chapter 7. Other Applications of Combinatorial Peptide Libraries 27. Chapter 8. Detailed Methodologies and Protocols 28. Polyphony 29. Selected CPLL Bibliography 30. Index
Low-Abundance Proteome Discovery addresses the most critical challenge in biomarker discovery and progress: the identification of low-abundance proteins. The book describes an original strategy developed by the authors that permits the detection of protein species typically found in very low abundance and that may yield valuable clues to future discoveries. Known as combinatorial peptide ligand libraries, these new methodologies are one of the hottest topics related to the study of proteomics and have applications in medical diagnostics, food quality, and plant analysis. The book is written for university and industry scientists starting proteomic studies of complex matrices (e.g., biological fluids, biopsies, recalcitrant plant tissues, foodstuff, and beverage analysis), researchers doing wet chemistry, and graduate-level students in the areas of analytical and biochemistry, biology, and genetics.
1. Cover image 2. Title page 3. Table of Contents 4. Copyright 5. Acknowledgments 6. Preface 7. Biography 8. Abbreviations 9. Trade Names 10. Antiphon 11. Chapter 1. Introducing Low-Abundance Species in Proteome Analysis 12. Chapter 2. Chromatographic and Electrophoretic Prefractionation Tools in Proteome Analysis 13. Chapter 3. Current Low-Abundance Protein Access 14. Chapter 4. Low-Abundance Protein Access by Combinatorial Peptide Libraries 15. 4.1 Combinatorial Chemistry 16. 4.2 Combinatorial Peptide Libraries 17. 4.3 Preparation of Solid-phase Combinatorial Peptide Ligand Libraries 18. 4.4 Use of Peptide Ligand Libraries as a Means of Reducing Protein Dynamic Range Concentration 19. 4.5 Peptide Ligand Library Properties in Protein Capturing 20. 4.6 Elution Mechanism Diversity and Complexity 21. 4.7 Selection of Elution Processes as a Function of Analytical Methods 22. 4.8 Towards a two-dimensional protein capture? 23. 4.9 References 24. Chapter 5. Plant Proteomics and Food and Beverage Analysis via CPLL Capture 25. Chapter 6. Biomedical Involvements of Low-Abundance Proteins 26. Chapter 7. Other Applications of Combinatorial Peptide Libraries 27. Chapter 8. Detailed Methodologies and Protocols 28. Polyphony 29. Selected CPLL Bibliography 30. Index
