MCB: CAR T Cells: Development, Characterization and Applications, Volume 167 in the Methods in Cell Biology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of timely topics, including High-efficiency of genetic modification using CRISPR/Cpf1 system for engineered CAR T-cell therapy, Determination of the Biodistribution of Chimeric Antigen Receptor-Modified T Cells against CD19 in NSG Mice, Generation of CAR-T cells using lentiviral vectors, Generation of CAR T-cells using γ-retroviral vector, Flow cytometry detection and quantification of CAR T cells into solid tumors, Evaluation of CAR-T Cell Cytotoxicity: Real-Time Impedance-Based Analysis, and much more.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Series Page - p. iii 5. Copyright - p. iii 6. Contributors - p. iii 7. Chapter 1: High-efficiency of genetic modification using CRISPR/Cpf1 system for engineered CAR-T cell therapy - p. 1 8. Chapter 2: Determination of the biodistribution of chimeric antigen receptor-modified T cells against CD19 in NSG mice - p. 15 9. Chapter 3: Generation of CAR-T cells using lentiviral vectors - p. 43 10. Chapter 4: Phenotyping of CAR T cells - p. 71 11. Chapter 5: Evaluation of CAR-T cell cytotoxicity: Real-time impedance-based analysis - p. 81 12. Chapter 6: Flow cytometry detection and quantification of CAR T cells into solid tumors - p. 99 13. Chapter 7: Generation and functional characterization of CAR exosomes - p. 123 14. Chapter 8: Preclinical testing of CAR T cells in zebrafish xenografts - p. 133 15. Chapter 9: In vivo experimental mouse model to test CD19CAR T cells generated with different methods - p. 149 16. Chapter 10: Non-invasive fluorescence imaging for tracking immune cells in preclinical models of immunotherapy - p. 163 17. Chapter 11: Generation of CAR T-cells using γ-retroviral vector - p. 171 18. Chapter 12: Methods to monitor in vivo expansion and efficacy of CAR-T cells in preclinical models - p. 185 19. Chapter 13: Strategies for manufacturing cell therapy products aligned with patient needs - p. 203
MCB: CAR T Cells: Development, Characterization and Applications, Volume 167 in the Methods in Cell Biology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of timely topics, including High-efficiency of genetic modification using CRISPR/Cpf1 system for engineered CAR T-cell therapy, Determination of the Biodistribution of Chimeric Antigen Receptor-Modified T Cells against CD19 in NSG Mice, Generation of CAR-T cells using lentiviral vectors, Generation of CAR T-cells using γ-retroviral vector, Flow cytometry detection and quantification of CAR T cells into solid tumors, Evaluation of CAR-T Cell Cytotoxicity: Real-Time Impedance-Based Analysis, and much more.
1. Cover image - p. i 2. Title page - p. ii 3. Table of Contents - p. iii 4. Series Page - p. iii 5. Copyright - p. iii 6. Contributors - p. iii 7. Chapter 1: High-efficiency of genetic modification using CRISPR/Cpf1 system for engineered CAR-T cell therapy - p. 1 8. Chapter 2: Determination of the biodistribution of chimeric antigen receptor-modified T cells against CD19 in NSG mice - p. 15 9. Chapter 3: Generation of CAR-T cells using lentiviral vectors - p. 43 10. Chapter 4: Phenotyping of CAR T cells - p. 71 11. Chapter 5: Evaluation of CAR-T cell cytotoxicity: Real-time impedance-based analysis - p. 81 12. Chapter 6: Flow cytometry detection and quantification of CAR T cells into solid tumors - p. 99 13. Chapter 7: Generation and functional characterization of CAR exosomes - p. 123 14. Chapter 8: Preclinical testing of CAR T cells in zebrafish xenografts - p. 133 15. Chapter 9: In vivo experimental mouse model to test CD19CAR T cells generated with different methods - p. 149 16. Chapter 10: Non-invasive fluorescence imaging for tracking immune cells in preclinical models of immunotherapy - p. 163 17. Chapter 11: Generation of CAR T-cells using γ-retroviral vector - p. 171 18. Chapter 12: Methods to monitor in vivo expansion and efficacy of CAR-T cells in preclinical models - p. 185 19. Chapter 13: Strategies for manufacturing cell therapy products aligned with patient needs - p. 203
