Experimental Analysis of Enzyme Mechanism Using Isotope Effects, Volume 596, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this comprehensive update include Measurement of enzyme binding isotope effects, Chemical ligation and isotope labeling to locate dynamic effects, Measurement of heavy enzyme isotope effects, Extracting kinetic isotope effects from a global analysis of reaction progress curves, KIE of metabolic flux and enzymes, Solvent and Primary KIE on Flavin Enzymes, and The Rapid Determination of Primary Deuterium Isotope Effects on Enzyme-Catalyzed Proton Transfer at Carbon in 50/50 HOH/DOD.
Readers who are interested in applying or understanding this research will find useful methods currently used for measuring isotope effects on solution and enzyme reactions.
1. 1 Introduction - p. 460 2. 2 Modeling Steady-State Isotope Effects - p. 461 3. 3 An Example: DesII - p. 472 4. 4 Theory - p. 479 5. Acknowledgments - p. 496 6. Chapter Seventeen: Determining Carbon Kinetic Isotope Effects Using Headspace Analysis of Evolved CO2 - p. 499 7. Chapter Eighteen: Kinetic Isotope Effect Studies to Elucidate the Reaction Mechanism of RNA-Modifying Enzymes - p. 522 8. Chapter Nineteen: Measurement of Kinetic Isotope Effects by Continuously Monitoring Isotopologue Ratios Using NMR Spectroscopy - p. 546 9. Author Index - p. 573 10. Subject Index - p. 599
Experimental Analysis of Enzyme Mechanism Using Isotope Effects, Volume 596, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this comprehensive update include Measurement of enzyme binding isotope effects, Chemical ligation and isotope labeling to locate dynamic effects, Measurement of heavy enzyme isotope effects, Extracting kinetic isotope effects from a global analysis of reaction progress curves, KIE of metabolic flux and enzymes, Solvent and Primary KIE on Flavin Enzymes, and The Rapid Determination of Primary Deuterium Isotope Effects on Enzyme-Catalyzed Proton Transfer at Carbon in 50/50 HOH/DOD.
Readers who are interested in applying or understanding this research will find useful methods currently used for measuring isotope effects on solution and enzyme reactions.
1. 1 Introduction - p. 460 2. 2 Modeling Steady-State Isotope Effects - p. 461 3. 3 An Example: DesII - p. 472 4. 4 Theory - p. 479 5. Acknowledgments - p. 496 6. Chapter Seventeen: Determining Carbon Kinetic Isotope Effects Using Headspace Analysis of Evolved CO2 - p. 499 7. Chapter Eighteen: Kinetic Isotope Effect Studies to Elucidate the Reaction Mechanism of RNA-Modifying Enzymes - p. 522 8. Chapter Nineteen: Measurement of Kinetic Isotope Effects by Continuously Monitoring Isotopologue Ratios Using NMR Spectroscopy - p. 546 9. Author Index - p. 573 10. Subject Index - p. 599
