Mechanisms of DNA Recombination and Genome Rearrangements: Intersection between Homologous Recombination, DNA Replication and DNA Repair, Volume 601, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field.
Homologous genetic recombination remains the most enigmatic process in DNA metabolism. The molecular machines of recombination preserve the integrity of the genetic material in all organisms and generate genetic diversity in evolution. The same molecular machines that support genetic integrity by orchestrating accurate repair of the most deleterious DNA lesions, however, also promote survival of cancerous cells and emergence of radiation and chemotherapy resistance. This two-volume set offers a comprehensive set of cutting edge methods to study various aspects of homologous recombination and cellular processes that utilize the enzymatic machinery of recombination. The chapters are written by the leading researches and cover a broad range of topics from the basic molecular mechanisms of recombinational proteins and enzymes to emerging cellular techniques and drug discovery efforts.
1. Cover image - p. iv 2. Title page - p. iv 3. Table of Contents - p. iv 4. Copyright - p. iv 5. Contributors - p. xi 6. Preface - p. xv 7. Chapter One: Using the Flp Recombinase to Induce Site-Specific Protein–DNA Nicks - p. xvi 8. Chapter Two: A Proximity Ligation-Based Method for Quantitative Measurement of D-Loop Extension in S. cerevisiae - p. 25 9. Chapter Three: Inducing and Detecting Mitotic DNA Synthesis at Difficult-to-Replicate Loci - p. 44 10. Chapter Four: An Approach to Detect and Study DNA Double-Strand Break Repair by Transcript RNA Using a Spliced-Antisense RNA Template - p. 58 11. Chapter Five: Assaying Repair at DNA Nicks - p. 70 12. Chapter Six: Recovery of Alternative End-Joining Repair Products From Drosophila Embryos - p. 89 13. Chapter Seven: High-Throughput Analysis of DNA Break-Induced Chromosome Rearrangements by Amplicon Sequencing - p. 110 14. Chapter Eight: Assaying Mutations Associated With Gene Conversion Repair of a Double-Strand Break - p. 144 15. Chapter Nine: Investigation of Break-Induced Replication in Yeast - p. 160 16. Chapter Ten: Targeting Allostery with Avatars to Design Inhibitors Assessed by Cell Activity: Dissecting MRE11 Endo- and Exonuclease Activities - p. 203 17. Chapter Eleven: Integrating Experimental and In Silico HTS in the Discovery of Inhibitors of Protein–Nucleic Acid Interactions - p. 241 18. Chapter Twelve: Monitoring Recombination During Meiosis in Budding Yeast - p. 273 19. Chapter Thirteen: S1-seq Assay for Mapping Processed DNA Ends - p. 307 20. Chapter Fourteen: Methods for Controlled Protein Depletion to Study Protein Function during Meiosis - p. 330 21. Chapter Fifteen: Quantitative Methods to Investigate the 4D Dynamics of Heterochromatic Repair Sites in Drosophila Cells - p. 357 22. Chapter Sixteen: Analysis of Meiotic Double-Strand Break Initiation in Mammals - p. 389
Mechanisms of DNA Recombination and Genome Rearrangements: Intersection between Homologous Recombination, DNA Replication and DNA Repair, Volume 601, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field.
Homologous genetic recombination remains the most enigmatic process in DNA metabolism. The molecular machines of recombination preserve the integrity of the genetic material in all organisms and generate genetic diversity in evolution. The same molecular machines that support genetic integrity by orchestrating accurate repair of the most deleterious DNA lesions, however, also promote survival of cancerous cells and emergence of radiation and chemotherapy resistance. This two-volume set offers a comprehensive set of cutting edge methods to study various aspects of homologous recombination and cellular processes that utilize the enzymatic machinery of recombination. The chapters are written by the leading researches and cover a broad range of topics from the basic molecular mechanisms of recombinational proteins and enzymes to emerging cellular techniques and drug discovery efforts.
1. Cover image - p. iv 2. Title page - p. iv 3. Table of Contents - p. iv 4. Copyright - p. iv 5. Contributors - p. xi 6. Preface - p. xv 7. Chapter One: Using the Flp Recombinase to Induce Site-Specific Protein–DNA Nicks - p. xvi 8. Chapter Two: A Proximity Ligation-Based Method for Quantitative Measurement of D-Loop Extension in S. cerevisiae - p. 25 9. Chapter Three: Inducing and Detecting Mitotic DNA Synthesis at Difficult-to-Replicate Loci - p. 44 10. Chapter Four: An Approach to Detect and Study DNA Double-Strand Break Repair by Transcript RNA Using a Spliced-Antisense RNA Template - p. 58 11. Chapter Five: Assaying Repair at DNA Nicks - p. 70 12. Chapter Six: Recovery of Alternative End-Joining Repair Products From Drosophila Embryos - p. 89 13. Chapter Seven: High-Throughput Analysis of DNA Break-Induced Chromosome Rearrangements by Amplicon Sequencing - p. 110 14. Chapter Eight: Assaying Mutations Associated With Gene Conversion Repair of a Double-Strand Break - p. 144 15. Chapter Nine: Investigation of Break-Induced Replication in Yeast - p. 160 16. Chapter Ten: Targeting Allostery with Avatars to Design Inhibitors Assessed by Cell Activity: Dissecting MRE11 Endo- and Exonuclease Activities - p. 203 17. Chapter Eleven: Integrating Experimental and In Silico HTS in the Discovery of Inhibitors of Protein–Nucleic Acid Interactions - p. 241 18. Chapter Twelve: Monitoring Recombination During Meiosis in Budding Yeast - p. 273 19. Chapter Thirteen: S1-seq Assay for Mapping Processed DNA Ends - p. 307 20. Chapter Fourteen: Methods for Controlled Protein Depletion to Study Protein Function during Meiosis - p. 330 21. Chapter Fifteen: Quantitative Methods to Investigate the 4D Dynamics of Heterochromatic Repair Sites in Drosophila Cells - p. 357 22. Chapter Sixteen: Analysis of Meiotic Double-Strand Break Initiation in Mammals - p. 389
