Methods for Monitoring Mitochondrial Functions During Pathogen Infection, Volume 194 in the Methods in Cell Biology series highlights advances in the field, with this new volume presenting interesting chapters on timely topics, including Mitochondrial Labeling with Fluorescent Proteins for Enhanced Morphological Characterization and Visualization of Intercellular Mitochondrial Transfer, Visualizing mitochondrial electron transport chain complexes and super-complexes during infection of human macrophages with Legionella pneumophila, Quantitative assessment of mitochondrial membrane potential in macrophages in sepsis, Quantification of mitochondrial reactive oxygen species in macrophages during sepsis, and much more.
Additional chapters cover Quantification of intracellular and mitochondrial ATP content in macrophages during lipopolysaccharide-induced inflammatory response, Analysis of cytosolic mtDNA release during Staphylococcus Aureus infection, Monitoring mitochondria dynamics upon infection using a tomographic approach, A screening system to determine bacterial effects on MADCAM1 expression by high endothelial venule cells, Isolation of mitochondria from Mycobacteria infected cells, Monitoring mitochondrial localization of Mycobacterial proteins, and more.
1. Methods for Monitoring Mitochondrial Functions During Pathogen Infection - p. i 2. Cover image - p. i 3. Title page - p. i 4. Table of Contents - p. i 5. Series Page - p. i 6. Copyright - p. iv 7. Contributors - p. ix 8. Chapter One Labeling of mitochondria for detection of intercellular mitochondrial transfer - p. 2 9. Chapter Two Visualizing mitochondrial electron transport chain complexes and super-complexes during infection of human macrophages with Legionella pneumophila - p. 19 10. Chapter Three Quantitative assessment of mitochondrial membrane potential in macrophages in sepsis - p. 44 11. Chapter Four Quantification of mitochondrial reactive oxygen species in macrophages during sepsis - p. 60 12. Chapter Five Quantification of intracellular and mitochondrial ATP content in macrophages during lipopolysaccharide-induced inflammatory response - p. 78 13. Chapter Six Analysis of cytosolic mtDNA release during Staphylococcus aureus infection - p. 94 14. Chapter Seven Monitoring cellular dynamics upon infection using a holotomography-based approach - p. 110 15. Chapter Eight A screening system to determine the effect of bacterial metabolites on MAdCAM-1 expression by transformed endothelial sinusoidal cells - p. 119 16. Chapter Nine Monitoring the mitochondrial localization of mycobacterial proteins - p. 135 17. Chapter Ten Bacterial predators and BALOs: Growth protocol and relation with mitochondria - p. 152 18. Chapter Eleven Assessment of adhering and invading properties of Escherichia coli strains - p. 170
Methods for Monitoring Mitochondrial Functions During Pathogen Infection, Volume 194 in the Methods in Cell Biology series highlights advances in the field, with this new volume presenting interesting chapters on timely topics, including Mitochondrial Labeling with Fluorescent Proteins for Enhanced Morphological Characterization and Visualization of Intercellular Mitochondrial Transfer, Visualizing mitochondrial electron transport chain complexes and super-complexes during infection of human macrophages with Legionella pneumophila, Quantitative assessment of mitochondrial membrane potential in macrophages in sepsis, Quantification of mitochondrial reactive oxygen species in macrophages during sepsis, and much more.
Additional chapters cover Quantification of intracellular and mitochondrial ATP content in macrophages during lipopolysaccharide-induced inflammatory response, Analysis of cytosolic mtDNA release during Staphylococcus Aureus infection, Monitoring mitochondria dynamics upon infection using a tomographic approach, A screening system to determine bacterial effects on MADCAM1 expression by high endothelial venule cells, Isolation of mitochondria from Mycobacteria infected cells, Monitoring mitochondrial localization of Mycobacterial proteins, and more.
1. Methods for Monitoring Mitochondrial Functions During Pathogen Infection - p. i 2. Cover image - p. i 3. Title page - p. i 4. Table of Contents - p. i 5. Series Page - p. i 6. Copyright - p. iv 7. Contributors - p. ix 8. Chapter One Labeling of mitochondria for detection of intercellular mitochondrial transfer - p. 2 9. Chapter Two Visualizing mitochondrial electron transport chain complexes and super-complexes during infection of human macrophages with Legionella pneumophila - p. 19 10. Chapter Three Quantitative assessment of mitochondrial membrane potential in macrophages in sepsis - p. 44 11. Chapter Four Quantification of mitochondrial reactive oxygen species in macrophages during sepsis - p. 60 12. Chapter Five Quantification of intracellular and mitochondrial ATP content in macrophages during lipopolysaccharide-induced inflammatory response - p. 78 13. Chapter Six Analysis of cytosolic mtDNA release during Staphylococcus aureus infection - p. 94 14. Chapter Seven Monitoring cellular dynamics upon infection using a holotomography-based approach - p. 110 15. Chapter Eight A screening system to determine the effect of bacterial metabolites on MAdCAM-1 expression by transformed endothelial sinusoidal cells - p. 119 16. Chapter Nine Monitoring the mitochondrial localization of mycobacterial proteins - p. 135 17. Chapter Ten Bacterial predators and BALOs: Growth protocol and relation with mitochondria - p. 152 18. Chapter Eleven Assessment of adhering and invading properties of Escherichia coli strains - p. 170
