Methods for the study of microbial biofilms: Current Trends and Recent Advancements provides a detailed overview of the latest techniques for the study of biofilms. The book explores a wide range of methods for the characterization and study of cells within biofilms, including microscopy techniques, genomic approaches, transcriptomics, proteomic and proteogenomic tools. Computational and mathematical modelling are also covered.
Chapters investigate the physiology of biofilm cells, gene expression pathways related to the formation, maturation and dispersion of biofilms, and molecular factors governing the biofilm formation and development, providing an overview of techniques that can be used at the cellular and molecular level. This book provides a broad overview of the biofilm field, along with the range of techniques that can be used to study and better understand them.
1. Title of Book - p. i 2. Cover image - p. i 3. Title page - p. ii 4. Table of Contents - p. iii 5. Copyright - p. iv 6. List of contributors - p. xi 7. Preface - p. xv 8. 1. Microbial biofilms: occurrence and implications - p. 1 9. 2. In silico methods to study biofilms - p. 17 10. 3. Microscopic methods for biofilm analysis - p. 31 11. 4. In vitro assays to study biofilms - p. 61 12. 5. In vivo animal models in biofilm research - p. 79 13. 6. Genomic approaches for biofilm analysis and control - p. 99 14. 7. Transcriptomics approaches to study biofilm: quantitative polymerase chain reaction-based methods - p. 133 15. 8. Decoding biofilm complexity through transcriptomics: microarray and RNA sequencing approaches - p. 159 16. 9. Proteomics studies of biofilm: methods, therapeutics, and recommendations - p. 167 17. 10. Metabolomics studies in microbial biofilms - p. 203 18. 11. Metagenomics/population studies for biofilms - p. 229 19. 12. Proteogenomics approaches toward biofilm formation studies - p. 247 20. 13. Mathematical tools to study and understand the biofilms - p. 257 21. Index - p. 279
Methods for the study of microbial biofilms: Current Trends and Recent Advancements provides a detailed overview of the latest techniques for the study of biofilms. The book explores a wide range of methods for the characterization and study of cells within biofilms, including microscopy techniques, genomic approaches, transcriptomics, proteomic and proteogenomic tools. Computational and mathematical modelling are also covered.
Chapters investigate the physiology of biofilm cells, gene expression pathways related to the formation, maturation and dispersion of biofilms, and molecular factors governing the biofilm formation and development, providing an overview of techniques that can be used at the cellular and molecular level. This book provides a broad overview of the biofilm field, along with the range of techniques that can be used to study and better understand them.
1. Title of Book - p. i 2. Cover image - p. i 3. Title page - p. ii 4. Table of Contents - p. iii 5. Copyright - p. iv 6. List of contributors - p. xi 7. Preface - p. xv 8. 1. Microbial biofilms: occurrence and implications - p. 1 9. 2. In silico methods to study biofilms - p. 17 10. 3. Microscopic methods for biofilm analysis - p. 31 11. 4. In vitro assays to study biofilms - p. 61 12. 5. In vivo animal models in biofilm research - p. 79 13. 6. Genomic approaches for biofilm analysis and control - p. 99 14. 7. Transcriptomics approaches to study biofilm: quantitative polymerase chain reaction-based methods - p. 133 15. 8. Decoding biofilm complexity through transcriptomics: microarray and RNA sequencing approaches - p. 159 16. 9. Proteomics studies of biofilm: methods, therapeutics, and recommendations - p. 167 17. 10. Metabolomics studies in microbial biofilms - p. 203 18. 11. Metagenomics/population studies for biofilms - p. 229 19. 12. Proteogenomics approaches toward biofilm formation studies - p. 247 20. 13. Mathematical tools to study and understand the biofilms - p. 257 21. Index - p. 279
