Methods in Kidney Cell Biology, Volume 153, represents state-of-the-art techniques in renal research that are ideal for veterans, graduate students, postdoctoral fellows, and clinical scientists and principal investigators. Topics in the new release include Single glomerular proteomics – a novel method in translational glomerular cell biology, Measurement of cytosolic and intraciliary calcium in live cells, Differentiation of human kidney organoids from pluripotent stem cells, Quantifying autophagic flux in kidney tissue using structured illumination microscopy, the Generation of primary cells from ADPKD and normal human kidneys, ADPKD cell proliferation and Cl-dependent fluid secretion, In vitro cyst formation of ADPKD cells, and much more.
1. Cover image - p. iv 2. Title page - p. iv 3. Table of Contents - p. iv 4. Copyright - p. iv 5. Contributors - p. ix 6. Preface - p. xiii 7. Chapter 1: Generation of primary cells from ADPKD and normal human kidneys - p. xiv 8. Chapter 2: Measurement of cytoplasmic and cilioplasmic calcium in a single living cell - p. 23 9. Chapter 3: Application of physiological shear stress to renal tubular epithelial cells - p. 42 10. Chapter 4: ADPKD cell proliferation and Cl−-dependent fluid secretion - p. 67 11. Chapter 5: In vitro cyst formation of ADPKD cells - p. 92 12. Chapter 6: Engineering kidney tissues for polycystic kidney disease modeling and drug discovery - p. 111 13. Chapter 7: Differentiation of human kidney organoids from pluripotent stem cells - p. 132 14. Chapter 8: Studying Na+ and K+ channels in aldosterone-sensitive distal nephrons - p. 150 15. Chapter 9: Metanephric organ culture - p. 168 16. Chapter 10: Ex vivo kidney slice preparations as a model system to study signaling cascades in kidney epithelial cells - p. 183 17. Chapter 11: Analysis of primary cilia in renal tissue and cells - p. 203 18. Chapter 12: Quantifying autophagic flux in kidney tissue using structured illumination microscopy - p. 229 19. Chapter 13: Investigation of epigenetics in kidney cell biology - p. 253
Methods in Kidney Cell Biology, Volume 153, represents state-of-the-art techniques in renal research that are ideal for veterans, graduate students, postdoctoral fellows, and clinical scientists and principal investigators. Topics in the new release include Single glomerular proteomics – a novel method in translational glomerular cell biology, Measurement of cytosolic and intraciliary calcium in live cells, Differentiation of human kidney organoids from pluripotent stem cells, Quantifying autophagic flux in kidney tissue using structured illumination microscopy, the Generation of primary cells from ADPKD and normal human kidneys, ADPKD cell proliferation and Cl-dependent fluid secretion, In vitro cyst formation of ADPKD cells, and much more.
1. Cover image - p. iv 2. Title page - p. iv 3. Table of Contents - p. iv 4. Copyright - p. iv 5. Contributors - p. ix 6. Preface - p. xiii 7. Chapter 1: Generation of primary cells from ADPKD and normal human kidneys - p. xiv 8. Chapter 2: Measurement of cytoplasmic and cilioplasmic calcium in a single living cell - p. 23 9. Chapter 3: Application of physiological shear stress to renal tubular epithelial cells - p. 42 10. Chapter 4: ADPKD cell proliferation and Cl−-dependent fluid secretion - p. 67 11. Chapter 5: In vitro cyst formation of ADPKD cells - p. 92 12. Chapter 6: Engineering kidney tissues for polycystic kidney disease modeling and drug discovery - p. 111 13. Chapter 7: Differentiation of human kidney organoids from pluripotent stem cells - p. 132 14. Chapter 8: Studying Na+ and K+ channels in aldosterone-sensitive distal nephrons - p. 150 15. Chapter 9: Metanephric organ culture - p. 168 16. Chapter 10: Ex vivo kidney slice preparations as a model system to study signaling cascades in kidney epithelial cells - p. 183 17. Chapter 11: Analysis of primary cilia in renal tissue and cells - p. 203 18. Chapter 12: Quantifying autophagic flux in kidney tissue using structured illumination microscopy - p. 229 19. Chapter 13: Investigation of epigenetics in kidney cell biology - p. 253
