Methods in Kidney Cell Biology, Part B, Volume 154 represents state-of-the-art techniques in renal research that are ideal for veterans, graduate students, postdoctoral fellows, clinical scientists and principal investigators. Topics in the new release include Single glomerular proteomics – a novel method in translational glomerular cell biology, Measurement of cytosolic and intraciliary calcium in live cells, Differentiation of human kidney organoids from pluripotent stem cells, Quantifying autophagic flux in kidney tissue using structured illumination microscopy, the Generation of primary cells from ADPKD and normal human kidneys, ADPKD cell proliferation and Cl-dependent fluid secretion, In vitro cyst formation of ADPKD cells, and much more.
1. Cover image - p. iv 2. Title page - p. iv 3. Table of Contents - p. iv 4. Copyright - p. iv 5. Contributors - p. ix 6. Preface - p. xiii 7. Chapter 1: Single glomerular proteomics: A novel tool for translational glomerular cell biology - p. xiv 8. Chapter 2: Kidney harvesting and metabolite extraction for metabolomics studies in rodents - p. 14 9. Chapter 3: Optical tissue clearing and immunolabeling in kidney research - p. 29 10. Chapter 4: Urinary extracellular vesicles as a source of biomarkers reflecting renal cellular biology in human disease - p. 41 11. Chapter 5: Intravital visualization of the primary cilium, tubule flow, and innate immune cells in the kidney utilizing an abdominal window imaging approach - p. 65 12. Chapter 6: Novel fluorescence techniques to quantitate renal cell biology - p. 83 13. Chapter 7: Manipulation of renal gene expression using oligonucleotides - p. 107 14. Chapter 8: Methods for renal lineage tracing: In vivo and beyond - p. 120 15. Chapter 9: In vivo magnetic resonance imaging techniques for structural and functional characterization of murine model kidneys - p. 143 16. Chapter 10: In vivo analysis of renal epithelial cells in zebrafish - p. 161 17. Chapter 11: Visualizing gene expression during zebrafish pronephros development and regeneration - p. 181 18. Chapter 12: Drosophila melanogaster and its nephrocytes: A versatile model for glomerular research - p. 215
Methods in Kidney Cell Biology, Part B, Volume 154 represents state-of-the-art techniques in renal research that are ideal for veterans, graduate students, postdoctoral fellows, clinical scientists and principal investigators. Topics in the new release include Single glomerular proteomics – a novel method in translational glomerular cell biology, Measurement of cytosolic and intraciliary calcium in live cells, Differentiation of human kidney organoids from pluripotent stem cells, Quantifying autophagic flux in kidney tissue using structured illumination microscopy, the Generation of primary cells from ADPKD and normal human kidneys, ADPKD cell proliferation and Cl-dependent fluid secretion, In vitro cyst formation of ADPKD cells, and much more.
1. Cover image - p. iv 2. Title page - p. iv 3. Table of Contents - p. iv 4. Copyright - p. iv 5. Contributors - p. ix 6. Preface - p. xiii 7. Chapter 1: Single glomerular proteomics: A novel tool for translational glomerular cell biology - p. xiv 8. Chapter 2: Kidney harvesting and metabolite extraction for metabolomics studies in rodents - p. 14 9. Chapter 3: Optical tissue clearing and immunolabeling in kidney research - p. 29 10. Chapter 4: Urinary extracellular vesicles as a source of biomarkers reflecting renal cellular biology in human disease - p. 41 11. Chapter 5: Intravital visualization of the primary cilium, tubule flow, and innate immune cells in the kidney utilizing an abdominal window imaging approach - p. 65 12. Chapter 6: Novel fluorescence techniques to quantitate renal cell biology - p. 83 13. Chapter 7: Manipulation of renal gene expression using oligonucleotides - p. 107 14. Chapter 8: Methods for renal lineage tracing: In vivo and beyond - p. 120 15. Chapter 9: In vivo magnetic resonance imaging techniques for structural and functional characterization of murine model kidneys - p. 143 16. Chapter 10: In vivo analysis of renal epithelial cells in zebrafish - p. 161 17. Chapter 11: Visualizing gene expression during zebrafish pronephros development and regeneration - p. 181 18. Chapter 12: Drosophila melanogaster and its nephrocytes: A versatile model for glomerular research - p. 215
