Mitosis and Meiosis, Part A, Volume 144, a new volume in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Unique to this updated volume are chapters on Analyzing the Spindle Assembly Checkpoint in human cell culture, an Analysis of CIN, a Functional analysis of the tubulin code in mitosis, Employing CRISPR/Cas9 genome engineering to dissect the molecular requirements for mitosis, Applying the auxin-inducible degradation (AID) system for rapid protein depletion in mammalian cells, Small Molecule Tools in Mitosis Research, Optogenetic control of mitosis with photocaged chemical, and more.
1. Cover image - p. iv 2. Title page - p. iv 3. Table of Contents - p. iv 4. Copyright - p. iv 5. Contributors - p. xiii 6. Preface - p. xix 7. Chapter 1: Assays for the spindle assembly checkpoint in cell culture - p. xix 8. Chapter 2: Quantitative methods to measure aneuploidy and chromosomal instability - p. 13 9. Chapter 3: Dissecting the role of the tubulin code in mitosis - p. 32 10. Chapter 4: Employing CRISPR/Cas9 genome engineering to dissect the molecular requirements for mitosis - p. 74 11. Chapter 5: Applying the auxin-inducible degradation system for rapid protein depletion in mammalian cells - p. 105 12. Chapter 6: Small molecule tools in mitosis research - p. 135 13. Chapter 7: Optogenetic control of mitosis with photocaged chemical dimerizers - p. 155 14. Chapter 8: Measuring mitotic forces - p. 164 15. Chapter 9: Employing the one-cell C. elegans embryo to study cell division processes - p. 183 16. Chapter 10: A cell-free system of Drosophila egg explants supporting native mitotic cycles - p. 231 17. Chapter 11: Living Xenopus oocytes, eggs, and embryos as models for cell division - p. 256 18. Chapter 12: Analysis of chromosomes from mouse oocytes and mammalian cultured cells by light microscopy - p. 284 19. Chapter 13: In vitro reconstitution of lateral to end-on conversion of kinetochore–microtubule attachments - p. 304 20. Chapter 14: Isolation of mitotic chromosomes from vertebrate cells and characterization of their proteome by mass spectrometry - p. 327 21. Chapter 15: Purification of kinetochores from the budding yeast Saccharomyces cerevisiae - p. 348 22. Chapter 16: Characterization of DNA helicases and nucleases from meiotic extracts of S. cerevisiae - p. 370 23. Chapter 17: Single-nucleus Hi-C of mammalian oocytes and zygotes - p. 388 24. Chapter 18: Induction of fetal primary oocytes and the meiotic prophase from mouse pluripotent stem cells - p. 406 25. Chapter 19: Electrical-assisted microinjection for analysis of fertilization and cell division in mammalian oocytes and early embryos - p. 429 26. Chapter 20: Single cell genomics to study DNA and chromosome changes in human gametes and embryos - p. 440 27. Chapter 21: Cytoplasmic removal, enucleation, and cell fusion of mouse oocytes - p. 457
Mitosis and Meiosis, Part A, Volume 144, a new volume in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Unique to this updated volume are chapters on Analyzing the Spindle Assembly Checkpoint in human cell culture, an Analysis of CIN, a Functional analysis of the tubulin code in mitosis, Employing CRISPR/Cas9 genome engineering to dissect the molecular requirements for mitosis, Applying the auxin-inducible degradation (AID) system for rapid protein depletion in mammalian cells, Small Molecule Tools in Mitosis Research, Optogenetic control of mitosis with photocaged chemical, and more.
1. Cover image - p. iv 2. Title page - p. iv 3. Table of Contents - p. iv 4. Copyright - p. iv 5. Contributors - p. xiii 6. Preface - p. xix 7. Chapter 1: Assays for the spindle assembly checkpoint in cell culture - p. xix 8. Chapter 2: Quantitative methods to measure aneuploidy and chromosomal instability - p. 13 9. Chapter 3: Dissecting the role of the tubulin code in mitosis - p. 32 10. Chapter 4: Employing CRISPR/Cas9 genome engineering to dissect the molecular requirements for mitosis - p. 74 11. Chapter 5: Applying the auxin-inducible degradation system for rapid protein depletion in mammalian cells - p. 105 12. Chapter 6: Small molecule tools in mitosis research - p. 135 13. Chapter 7: Optogenetic control of mitosis with photocaged chemical dimerizers - p. 155 14. Chapter 8: Measuring mitotic forces - p. 164 15. Chapter 9: Employing the one-cell C. elegans embryo to study cell division processes - p. 183 16. Chapter 10: A cell-free system of Drosophila egg explants supporting native mitotic cycles - p. 231 17. Chapter 11: Living Xenopus oocytes, eggs, and embryos as models for cell division - p. 256 18. Chapter 12: Analysis of chromosomes from mouse oocytes and mammalian cultured cells by light microscopy - p. 284 19. Chapter 13: In vitro reconstitution of lateral to end-on conversion of kinetochore–microtubule attachments - p. 304 20. Chapter 14: Isolation of mitotic chromosomes from vertebrate cells and characterization of their proteome by mass spectrometry - p. 327 21. Chapter 15: Purification of kinetochores from the budding yeast Saccharomyces cerevisiae - p. 348 22. Chapter 16: Characterization of DNA helicases and nucleases from meiotic extracts of S. cerevisiae - p. 370 23. Chapter 17: Single-nucleus Hi-C of mammalian oocytes and zygotes - p. 388 24. Chapter 18: Induction of fetal primary oocytes and the meiotic prophase from mouse pluripotent stem cells - p. 406 25. Chapter 19: Electrical-assisted microinjection for analysis of fertilization and cell division in mammalian oocytes and early embryos - p. 429 26. Chapter 20: Single cell genomics to study DNA and chromosome changes in human gametes and embryos - p. 440 27. Chapter 21: Cytoplasmic removal, enucleation, and cell fusion of mouse oocytes - p. 457
