Mitosis and Meiosis, Part B, Volume 145, a new volume in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Unique to this updated volume are chapters on Mitotic live cell imaging at different time scales, the characterization of mitotic spindle by multi-mode correlative microscopy, STED microscopy of mitosis, Correlating light microscopy with serial block face scanning electron microscopy to study mitotic spindle architecture, quantification of three-dimensional spindle architecture, Imaging based assays for mitotic chromosome condensation and dynamics, and more.
1. Cover image - p. iv 2. Title page - p. iv 3. Table of Contents - p. iv 4. Copyright - p. iv 5. Contributors - p. xi 6. Preface - p. xvii 7. Chapter 1: Mitotic live-cell imaging at different timescales - p. xvii 8. Chapter 2: Correlating light microscopy with serial block face scanning electron microscopy to study mitotic spindle architecture - p. 27 9. Chapter 3: Quantification of three-dimensional spindle architecture - p. 43 10. Chapter 4: Quantitative live and super-resolution microscopy of mitotic chromosomes - p. 64 11. Chapter 5: Live imaging of cell division in 3D stem-cell organoid cultures - p. 90 12. Chapter 6: Choosing the right microscope to image mitosis in zebrafish embryos: A practical guide - p. 106 13. Chapter 7: Advanced microscopy methods for bioimaging of mitotic microtubules in plants - p. 127 14. Chapter 8: Microsurgery and microinjection techniques in mitosis research - p. 158 15. Chapter 9: Analyzing the micromechanics of the cell division apparatus - p. 172 16. Chapter 10: Optogenetic reversible knocksideways, laser ablation, and photoactivation on the mitotic spindle in human cells - p. 189 17. Chapter 11: Live imaging of C. elegans oocytes and early embryos - p. 215 18. Chapter 12: Combining microscopy and biochemistry to study meiotic spindle assembly in Drosophila oocytes - p. 236 19. Chapter 13: Methods to study meiosis in insect spermatocytes - p. 248 20. Chapter 14: Live-cell microscopy of meiosis in spermatocytes - p. 267 21. Chapter 15: Live imaging of cell division in preimplantation mouse embryos using inverted light-sheet microscopy - p. 277 22. Chapter 16: Correlated light and electron microscopy of cell division in large marine oocytes, eggs, and embryos - p. 292 23. Chapter 17: A microscopy-based approach for studying meiosis in live and fixed human oocytes - p. 313 24. Chapter 18: Superresolution imaging of the synaptonemal complex - p. 333 25. Chapter 19: Cryo-electron tomography of SYCP3 fibers under native conditions - p. 346
Mitosis and Meiosis, Part B, Volume 145, a new volume in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Unique to this updated volume are chapters on Mitotic live cell imaging at different time scales, the characterization of mitotic spindle by multi-mode correlative microscopy, STED microscopy of mitosis, Correlating light microscopy with serial block face scanning electron microscopy to study mitotic spindle architecture, quantification of three-dimensional spindle architecture, Imaging based assays for mitotic chromosome condensation and dynamics, and more.
1. Cover image - p. iv 2. Title page - p. iv 3. Table of Contents - p. iv 4. Copyright - p. iv 5. Contributors - p. xi 6. Preface - p. xvii 7. Chapter 1: Mitotic live-cell imaging at different timescales - p. xvii 8. Chapter 2: Correlating light microscopy with serial block face scanning electron microscopy to study mitotic spindle architecture - p. 27 9. Chapter 3: Quantification of three-dimensional spindle architecture - p. 43 10. Chapter 4: Quantitative live and super-resolution microscopy of mitotic chromosomes - p. 64 11. Chapter 5: Live imaging of cell division in 3D stem-cell organoid cultures - p. 90 12. Chapter 6: Choosing the right microscope to image mitosis in zebrafish embryos: A practical guide - p. 106 13. Chapter 7: Advanced microscopy methods for bioimaging of mitotic microtubules in plants - p. 127 14. Chapter 8: Microsurgery and microinjection techniques in mitosis research - p. 158 15. Chapter 9: Analyzing the micromechanics of the cell division apparatus - p. 172 16. Chapter 10: Optogenetic reversible knocksideways, laser ablation, and photoactivation on the mitotic spindle in human cells - p. 189 17. Chapter 11: Live imaging of C. elegans oocytes and early embryos - p. 215 18. Chapter 12: Combining microscopy and biochemistry to study meiotic spindle assembly in Drosophila oocytes - p. 236 19. Chapter 13: Methods to study meiosis in insect spermatocytes - p. 248 20. Chapter 14: Live-cell microscopy of meiosis in spermatocytes - p. 267 21. Chapter 15: Live imaging of cell division in preimplantation mouse embryos using inverted light-sheet microscopy - p. 277 22. Chapter 16: Correlated light and electron microscopy of cell division in large marine oocytes, eggs, and embryos - p. 292 23. Chapter 17: A microscopy-based approach for studying meiosis in live and fixed human oocytes - p. 313 24. Chapter 18: Superresolution imaging of the synaptonemal complex - p. 333 25. Chapter 19: Cryo-electron tomography of SYCP3 fibers under native conditions - p. 346
