Monitoring Vesicular Trafficking in Cellular Responses to Stress - Part B, Volume 165 in the Methods in Cell Biology series, highlights new advances in the field, with this new release presenting coverage of different topics, including A novel tool for detecting lysosomal membrane permeabilization by high-throughput fluorescence microscopy, Exploring selective autophagy in Drosophila, Assessment of EGFP-Q74 degradation for the measurement of autophagic flux, Multimodal assessment of autophagy in mammalian cells with a novel, LC3-based tandem reporter, Multiplex quantification of autophagic flux by imaging flow cytometry, Monitoring autophagic flux in Caenorhabditis elegans using p62/SQST-1 reporters, High throughput screening for autophagy, and much more.
1. Cover image - p. page_i 2. Title page - p. page_i 3. Table of Contents - p. page_i 4. Copyright - p. page_iv 5. Contributors - p. page_xi 6. Vesicular trafficking in cellular responses to stress - p. page_xix 7. Chapter 1: A novel tool for detecting lysosomal membrane permeabilization by high-throughput fluorescence microscopy - p. page_1 8. Chapter 2: Exploring selective autophagy in Drosophila: Methods to identify Atg8-interacting proteins - p. page_13 9. Chapter 3: Assessment of EGFP-Q74 degradation for the measurement of autophagic flux - p. page_31 10. Chapter 4: Multimodal assessment of autophagy in mammalian cells with a novel, LC3-based tandem reporter - p. page_39 11. Chapter 5: Multiplexed quantification of autophagic flux by imaging flow cytometry - p. page_59 12. Chapter 6: Monitoring autophagic flux in Caenorhabditis elegans using a p62/SQST-1 reporter - p. page_73 13. Chapter 7: High throughput screening for autophagy - p. page_89 14. Chapter 8: Determination of mitophagy by electron microscope - p. page_103 15. Chapter 9: Quantification of intracellular ACBP/DBI levels - p. page_111 16. Chapter 10: Immunoblot-based assays for assessing autophagy in the turquoise killifish Nothobranchius furzeri - p. page_123 17. Chapter 11: Monitoring autophagy using super-resolution structured illumination and direct stochastic optical reconstruction microscopy - p. page_139 18. Chapter 12: Monitoring selective autophagy of mitochondria using super-resolution microscopy - p. page_153 19. Chapter 13: Monitoring autophagy-dependent ferroptosis - p. page_163 20. Chapter 14: Quantitative analysis of autophagy-related protein LC3B by quantum-dot-based molecular imaging - p. page_177 21. Chapter 15: Study of microRNAs carried by exosomes - p. page_187 22. Chapter 16: Simultaneous determination of intraluminal lysosomal calcium and pH by dextran-conjugated fluorescent dyes - p. page_199
Monitoring Vesicular Trafficking in Cellular Responses to Stress - Part B, Volume 165 in the Methods in Cell Biology series, highlights new advances in the field, with this new release presenting coverage of different topics, including A novel tool for detecting lysosomal membrane permeabilization by high-throughput fluorescence microscopy, Exploring selective autophagy in Drosophila, Assessment of EGFP-Q74 degradation for the measurement of autophagic flux, Multimodal assessment of autophagy in mammalian cells with a novel, LC3-based tandem reporter, Multiplex quantification of autophagic flux by imaging flow cytometry, Monitoring autophagic flux in Caenorhabditis elegans using p62/SQST-1 reporters, High throughput screening for autophagy, and much more.
1. Cover image - p. page_i 2. Title page - p. page_i 3. Table of Contents - p. page_i 4. Copyright - p. page_iv 5. Contributors - p. page_xi 6. Vesicular trafficking in cellular responses to stress - p. page_xix 7. Chapter 1: A novel tool for detecting lysosomal membrane permeabilization by high-throughput fluorescence microscopy - p. page_1 8. Chapter 2: Exploring selective autophagy in Drosophila: Methods to identify Atg8-interacting proteins - p. page_13 9. Chapter 3: Assessment of EGFP-Q74 degradation for the measurement of autophagic flux - p. page_31 10. Chapter 4: Multimodal assessment of autophagy in mammalian cells with a novel, LC3-based tandem reporter - p. page_39 11. Chapter 5: Multiplexed quantification of autophagic flux by imaging flow cytometry - p. page_59 12. Chapter 6: Monitoring autophagic flux in Caenorhabditis elegans using a p62/SQST-1 reporter - p. page_73 13. Chapter 7: High throughput screening for autophagy - p. page_89 14. Chapter 8: Determination of mitophagy by electron microscope - p. page_103 15. Chapter 9: Quantification of intracellular ACBP/DBI levels - p. page_111 16. Chapter 10: Immunoblot-based assays for assessing autophagy in the turquoise killifish Nothobranchius furzeri - p. page_123 17. Chapter 11: Monitoring autophagy using super-resolution structured illumination and direct stochastic optical reconstruction microscopy - p. page_139 18. Chapter 12: Monitoring selective autophagy of mitochondria using super-resolution microscopy - p. page_153 19. Chapter 13: Monitoring autophagy-dependent ferroptosis - p. page_163 20. Chapter 14: Quantitative analysis of autophagy-related protein LC3B by quantum-dot-based molecular imaging - p. page_177 21. Chapter 15: Study of microRNAs carried by exosomes - p. page_187 22. Chapter 16: Simultaneous determination of intraluminal lysosomal calcium and pH by dextran-conjugated fluorescent dyes - p. page_199
