Next Generation Sequencing (NGS) Technology in DNA Analysis explains and summarizes next generation sequencing (NGS) technological applications in the field of forensic DNA analysis. The book covers the transition from capillary electrophoresis (CE)-based technique to NGS platforms and the fundamentals of NGS technologies, applications, and advances. Sections provide an overview of NGS technology and forensic science, including information on processing biological samples for forensic analysis, sequence analysis, and data analysis software as well as the analysis of NGS data. The book explores the valuable applications of NGS-based forensic DNA analysis and covers the validations and interpretation guidelines of NGS workflows.
With chapter contributions from an international array of experts and the inclusion of practical case studies, this book is a useful reference for academicians and researchers in genetics, biotechnology, bioinformatics, biology, and medicine as well as forensic DNA scientists and practitioners who aim to learn, use, apply, and validate NGS-based technologies.
1. Title of Book - p. i 2. Cover image - p. i 3. Title page - p. ii 4. Table of Contents - p. iii 5. Copyright - p. iii 6. Dedications - p. iii 7. Contributors - p. xvii 8. Preface - p. xx 9. Acknowledgments - p. xx 10. Section 1: Useful applications of NGS-based forensic DNA analysis - p. xx 11. Chapter 1. Transition of capillary electrophoresis to next generation sequencing for forensic DNA analysis: Need of the hour - p. 3 12. Chapter 2. Using conventional STR technology in analyzing biological transfer evidence - p. 21 13. Chapter 3. Overview of NGS platforms and technological advancements for forensic applications - p. 35 14. Chapter 4. Processing of biological samples for forensic NGS analysis - p. 59 15. Chapter 5. Commercial kits commonly used for NGS based forensic DNA analysis - p. 73 16. Chapter 6. Applications of nanopore sequencing for forensic analysis - p. 85 17. Chapter 7. Microhaplotypes analysis for human identification using next-generation sequencing (NGS) - p. 99 18. Chapter 8. Tools and techniques of using NGS platforms in forensic population genetic studies - p. 121 19. Section 2: Useful applications of NGS-based forensic DNA analysis - p. 171 20. Chapter 9. Applications of NGS in analysis of autosomal STRs - p. 175 21. Chapter 10. Differentiating monozygotic twins using NGS - p. 185 22. Chapter 11. Application of NGS in maternal genome analysis in ancient human remains - p. 195 23. Chapter 12. Application of NGS technology for parentage testing and relatedness analysis - p. 219 24. Chapter 13. Forensic relevance of SNP analysis in next-generation sequencing - p. 243 25. Chapter 14. Forensic analysis of hair using NGS technology - p. 279 26. Chapter 15. Forensic DNA phenotyping using next-generation sequencing - p. 289 27. Chapter 16. Forensic DNA phenotyping in the next-generation sequencing era - p. 311 28. Chapter 17. Forensic applications of epigenetic (DNA methylation) markers through NGS - p. 337 29. Chapter 18. Forensic applications of NGS-based microRNA analysis - p. 357 30. Chapter 19. NGS profiling of water and soil microbial DNA in forensic science - p. 375 31. Chapter 20. Applications of NGS in analysis of challenging samples - p. 387 32. Chapter 21. Detection of human body fluid through mRNA analysis using NGS - p. 413 33. Chapter 22. NGS-based detection and differentiation of forensically relevant body fluids using conventional, molecular, and microbial techniques - p. 425 34. Chapter 23. Application of next-generation sequencing technology and investigative genetic genealogy to solving cold cases - p. 451 35. Section 3: Challenges in implementing NGS in forensic laboratories - p. 468 36. Chapter 24. Troubleshooting and challenges of Next-generation sequencing technology in forensic use - p. 471 37. Chapter 25. Validation of NGS for casework at forensic DNA laboratories - p. 485 38. Chapter 26. Challenges in using genetic genealogy in forensics - p. 511 39. Chapter 27. The role of artificial intelligence and machine learning in NGS - p. 531 40. Chapter 28. Ethical issues of forensic application of NGS technology - p. 539 41. Chapter 29. Ethical, legal, and social challenges of next-generation sequencing technologies (NGS) in forensic criminal identification - p. 551 42. Index - p. 571
Next Generation Sequencing (NGS) Technology in DNA Analysis explains and summarizes next generation sequencing (NGS) technological applications in the field of forensic DNA analysis. The book covers the transition from capillary electrophoresis (CE)-based technique to NGS platforms and the fundamentals of NGS technologies, applications, and advances. Sections provide an overview of NGS technology and forensic science, including information on processing biological samples for forensic analysis, sequence analysis, and data analysis software as well as the analysis of NGS data. The book explores the valuable applications of NGS-based forensic DNA analysis and covers the validations and interpretation guidelines of NGS workflows.
With chapter contributions from an international array of experts and the inclusion of practical case studies, this book is a useful reference for academicians and researchers in genetics, biotechnology, bioinformatics, biology, and medicine as well as forensic DNA scientists and practitioners who aim to learn, use, apply, and validate NGS-based technologies.
1. Title of Book - p. i 2. Cover image - p. i 3. Title page - p. ii 4. Table of Contents - p. iii 5. Copyright - p. iii 6. Dedications - p. iii 7. Contributors - p. xvii 8. Preface - p. xx 9. Acknowledgments - p. xx 10. Section 1: Useful applications of NGS-based forensic DNA analysis - p. xx 11. Chapter 1. Transition of capillary electrophoresis to next generation sequencing for forensic DNA analysis: Need of the hour - p. 3 12. Chapter 2. Using conventional STR technology in analyzing biological transfer evidence - p. 21 13. Chapter 3. Overview of NGS platforms and technological advancements for forensic applications - p. 35 14. Chapter 4. Processing of biological samples for forensic NGS analysis - p. 59 15. Chapter 5. Commercial kits commonly used for NGS based forensic DNA analysis - p. 73 16. Chapter 6. Applications of nanopore sequencing for forensic analysis - p. 85 17. Chapter 7. Microhaplotypes analysis for human identification using next-generation sequencing (NGS) - p. 99 18. Chapter 8. Tools and techniques of using NGS platforms in forensic population genetic studies - p. 121 19. Section 2: Useful applications of NGS-based forensic DNA analysis - p. 171 20. Chapter 9. Applications of NGS in analysis of autosomal STRs - p. 175 21. Chapter 10. Differentiating monozygotic twins using NGS - p. 185 22. Chapter 11. Application of NGS in maternal genome analysis in ancient human remains - p. 195 23. Chapter 12. Application of NGS technology for parentage testing and relatedness analysis - p. 219 24. Chapter 13. Forensic relevance of SNP analysis in next-generation sequencing - p. 243 25. Chapter 14. Forensic analysis of hair using NGS technology - p. 279 26. Chapter 15. Forensic DNA phenotyping using next-generation sequencing - p. 289 27. Chapter 16. Forensic DNA phenotyping in the next-generation sequencing era - p. 311 28. Chapter 17. Forensic applications of epigenetic (DNA methylation) markers through NGS - p. 337 29. Chapter 18. Forensic applications of NGS-based microRNA analysis - p. 357 30. Chapter 19. NGS profiling of water and soil microbial DNA in forensic science - p. 375 31. Chapter 20. Applications of NGS in analysis of challenging samples - p. 387 32. Chapter 21. Detection of human body fluid through mRNA analysis using NGS - p. 413 33. Chapter 22. NGS-based detection and differentiation of forensically relevant body fluids using conventional, molecular, and microbial techniques - p. 425 34. Chapter 23. Application of next-generation sequencing technology and investigative genetic genealogy to solving cold cases - p. 451 35. Section 3: Challenges in implementing NGS in forensic laboratories - p. 468 36. Chapter 24. Troubleshooting and challenges of Next-generation sequencing technology in forensic use - p. 471 37. Chapter 25. Validation of NGS for casework at forensic DNA laboratories - p. 485 38. Chapter 26. Challenges in using genetic genealogy in forensics - p. 511 39. Chapter 27. The role of artificial intelligence and machine learning in NGS - p. 531 40. Chapter 28. Ethical issues of forensic application of NGS technology - p. 539 41. Chapter 29. Ethical, legal, and social challenges of next-generation sequencing technologies (NGS) in forensic criminal identification - p. 551 42. Index - p. 571
