Optochemical Biology, Volume 624, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters on a variety of topics, including Cell Lineage Tracing in Zebrafish Embryos with an Expanded Genetic Code, Optical Control of Tumor Induction in the Zebrafish, Optogenetic Control by Pulsed Illumination, Optimizing the Photocontrol of bZIP Coiled Coils with Azobenzene Crosslinkers: Role of the Crosslinking Site, Site-Directed RNA Editing in Vivo Can Be Triggered by the Light-Driven Assembly of an Artificial Riboprotein, In Situ Formation of an Azo Bridge on Proteins Controllable by Visible Light, and much more.
1. Cover image - p. iv 2. Title page - p. iv 3. Table of Contents - p. iv 4. Copyright - p. iv 5. Contributors - p. ix 6. Preface - p. xiii 7. Chapter One: Optical control of protein activity and gene expression by photoactivation of caged cyclofen - p. xv 8. Chapter Two: Reversible optogenetic control of protein function and localization - p. 23 9. Chapter Three: Protocols for the generation of caged guideRNAs for light-triggered RNA-targeting with SNAP-ADARs - p. 45 10. Chapter Four: Combinatorial control of gene function with wavelength-selective caged morpholinos - p. 68 11. Chapter Five: Phosphodiester photo-tethers for the (multi-)cyclic conformational caging of oligonucleotides - p. 88 12. Chapter Six: Chemical modification of proteins with photocleavable groups - p. 111 13. Chapter Seven: Intramolecular cross-linking of proteins with azobenzene-based cross-linkers - p. 128 14. Chapter Eight: Ruthenium-cross-linked hydrogels for rapid, visible-light protein release - p. 149 15. Chapter Nine: Chromatically independent, two-color uncaging of glutamate and GABA with one- or two-photon excitation - p. 166 16. Chapter Ten: DIY optogenetics: Building, programming, and using the Light Plate Apparatus - p. 196 17. Chapter Eleven: Pulsatile illumination for photobiology and optogenetics - p. 226 18. Chapter Twelve: Genetically encoding photoswitchable click amino acids for general optical control of conformation and function of proteins - p. 248 19. Chapter Thirteen: Light-activation of Cre recombinase in zebrafish embryos through genetic code expansion - p. 264
Optochemical Biology, Volume 624, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters on a variety of topics, including Cell Lineage Tracing in Zebrafish Embryos with an Expanded Genetic Code, Optical Control of Tumor Induction in the Zebrafish, Optogenetic Control by Pulsed Illumination, Optimizing the Photocontrol of bZIP Coiled Coils with Azobenzene Crosslinkers: Role of the Crosslinking Site, Site-Directed RNA Editing in Vivo Can Be Triggered by the Light-Driven Assembly of an Artificial Riboprotein, In Situ Formation of an Azo Bridge on Proteins Controllable by Visible Light, and much more.
1. Cover image - p. iv 2. Title page - p. iv 3. Table of Contents - p. iv 4. Copyright - p. iv 5. Contributors - p. ix 6. Preface - p. xiii 7. Chapter One: Optical control of protein activity and gene expression by photoactivation of caged cyclofen - p. xv 8. Chapter Two: Reversible optogenetic control of protein function and localization - p. 23 9. Chapter Three: Protocols for the generation of caged guideRNAs for light-triggered RNA-targeting with SNAP-ADARs - p. 45 10. Chapter Four: Combinatorial control of gene function with wavelength-selective caged morpholinos - p. 68 11. Chapter Five: Phosphodiester photo-tethers for the (multi-)cyclic conformational caging of oligonucleotides - p. 88 12. Chapter Six: Chemical modification of proteins with photocleavable groups - p. 111 13. Chapter Seven: Intramolecular cross-linking of proteins with azobenzene-based cross-linkers - p. 128 14. Chapter Eight: Ruthenium-cross-linked hydrogels for rapid, visible-light protein release - p. 149 15. Chapter Nine: Chromatically independent, two-color uncaging of glutamate and GABA with one- or two-photon excitation - p. 166 16. Chapter Ten: DIY optogenetics: Building, programming, and using the Light Plate Apparatus - p. 196 17. Chapter Eleven: Pulsatile illumination for photobiology and optogenetics - p. 226 18. Chapter Twelve: Genetically encoding photoswitchable click amino acids for general optical control of conformation and function of proteins - p. 248 19. Chapter Thirteen: Light-activation of Cre recombinase in zebrafish embryos through genetic code expansion - p. 264
